Abstract：The interactions between Fc(COOH)2 (λmax=255 nm) and BSA (λmax=277.5 nm) were studied by UV-Vis spectroscopy. The results showed that, there was a good linear relationship between absorbance and concentration for Fc(COOH)2 in range of 10~190 μmol·L-1 and for BSA in range of 100~1 900 mg·L-1, respectively. After the reaction of Fc(COOH)2 and BSA , λmax shifted to 275 nm to show the existing reaction. When the concentration of Fc(COOH)2 or BSA was fixed, the absorbance of Fc(COOH)2 or BSA increased with the increase of BSA or Fc(COOH)2 concentration, which showed the hydrogen bonding form between Fc(COOH)2 and BSA and the growth of molecular chain, and the increase of absorbance was attributed to the fact that bigger molecule absorbs more energy and release. Meanwhile, the stability of new formed molecule bond was affected by reaction time, after reacted for 0.1 to 24 and 96 h, the absorbance at 275 nm changed from 1.062 to 1.045 and 0.986 for Fc(COOH)2 (70 μmol·L-1) and BSA (1 900 mg·L-1), and the absorbance changed from 0.813 to 0.794 and 0.750 for Fc(COOH)2 (190 μmol·L-1) and BSA (700 mg·L-1), respectively.
Key words：UV-Vis spectroscopy; Fc(COOH)2; BSA; Hydrogen bond
基金资助: The National Natural Science Foundation of China (61664014, 61627807, 81873913, 61301038), Natural Science Foundation of Guangxi Province (2015GXNSFBA139041)
通讯作者:
陈真诚
E-mail: chenzhcheng@163.com
作者简介: HAN Guo-cheng, (1981—), professor, School of Life and Environmental Sciences, Guilin University of Electronic Technology
e-mail:
hangc81@guet.edu.cn
引用本文:
韩国成，苏晓瑞，侯嘉婷，冯小珍，陈真诚. 紫外-可见光谱法研究Fc(COOH)2和BSA相互作用[J]. 光谱学与光谱分析, 2018, 38(12): 3958-3962.
HAN Guo-cheng, SU Xiao-rui, HOU Jia-ting, FENG Xiao-zhen, CHEN Zhen-cheng. Interaction Study of Fc(COOH)2 and BSA by UV-Vis Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(12): 3958-3962.
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