Research on DNA Fluorescence Capillary Biosensor Marked by Goldview
WANG Yan-jun1,LI Yong-sheng2*,YANG Quan-yu1,HUANG Yi1,TANG Jing1,GAO Xiu-feng1*
1. West China School of Preclinical and Forensic Medicine, Sichuan University， Chengdu 610041, China 2. School of Chemical Engineering, Sichuan University, Chengdu 610065, China
Abstract：Goldview marked DNA fluorescence capillary biosensor was studied in the present paper. Based on fluorescence capillary analysis (FCA), the DNA biosensor uses capillary as immobilization carrier and detection carrier of DNA probe. Probes (20-mer-ssDNA) were immobilized on the inner wall of capillary by poly-l-lysine, and DNA fluorescence capillary biosensor (DNA-FCB) was made. After being hybridized with complementary target DNA and dyed by Goldview, the target DNA was qualified or quantified by detecting the fluorescence density of the Goldview using F-4500 spectrofluorometer. The sample volume was 12 μL.The concentration of the target DNA showed good linearity with the fluorescence intensity in the range of 0.4-4 μmol·L-1 (2.4-24 mg·L-1)(y=65.911x+3.994 4，r=0.998 9). The RSD was lower than 3.5%. The concentration detection limit of the target DNA was 0.39 μmol·L-1(2.2 mg·L-1). The DNA-FCB can be used to qualify or quantify the target DNA. It’s advantages are simplicity of manipulation, thimbleful of sample and reagent volumes, repeated use of capillary, and the lowest test cost. By using DNA-FCB to qualify the target DNA, we can consumedly decrease the pollution of the environment.
