| 光谱学与光谱分析 |
|
|
|
|
|
| Spectroscopy Study on the Interaction of Colchicine and Human Serum Albumin |
| MA Jun-yan1,CHEN Ke-hai1,ZHENG Xue-fang1,2*,GUO Ming2,MA Jing1,TANG Qian1,WANG Yu-lian1, HU Jie-han2,3 |
1. College of Bioengineering, Dalian University, Dalian 116622, China
2. Liaoning Key Lab of Bio-organic Chemistry, Dalian University, Dalian 116622, China
3. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China |
|
|
|
|
Abstract The binding reaction of colchicine with human serum albumin(HSA) was studied by UV-Vis absorption, fluorescence and circular dichroism spectrometry. The results indicated that colchicine led to the increase in UV absorption and the quenching of intrinsic fluorescence of HSA. As the temperature increased, the quenching constant KSV decreased. The binding constants and the numbers of the binding sites of the interaction between colchicine and HSA at different temperatures were obtained. The thermodynamic parameters, enthalpy change (ΔH) and entropy change (ΔS), were calculated to be -11.66 kJ·mol-１ and 51.507 J(mol·K)-１ respectively according to Van’t Hoff equation, which suggested that the main binding force between colchicine and HSA was static interaction. The protein conformation was altered(CD date) with decreasing of α-helices in the presence of colchicine. The results showed that the quenching mechanism of the combination of colchicine with human serum albumin was a static quenching procedure.
|
|
Received: 2006-08-16
Accepted: 2006-11-08
|
|
|
|
Corresponding Authors:
ZHENG Xue-fang
E-mail: zhengxuefang@263.net
|
|
| Cite this article: |
|
MA Jun-yan,CHEN Ke-hai,ZHENG Xue-fang, et al. Spectroscopy Study on the Interaction of Colchicine and Human Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(12): 2485-2489.
|
|
|
|
| URL: |
|
https://www.gpxygpfx.com/EN/Y2007/V27/I12/2485 |
[1] Sullivan T P, King L E, Boyd A S. Journal of American Academy of Dermatology, 1998, 39(6): 993.
[2] Wolff J, Knipling L. Journal of Biological Chemistry, 1995, 270(28): 16809.
[3] LU Ji-xin, ZHANG Gui-zhu, ZHAO Peng, et al(卢继新，张贵珠，赵 鹏, 等). Acta Chimica Sinica(化学学报)，1997, 55: 915.
[4] LIU Yong-ming, LI Gui-zhi, SUN Xi-fen(刘永明，李桂枝，孙希芬). Chinese J. Anal. Chem.(分析化学)，2004，32(5): 615.
[5] Hu Yan-Jun, Liu Yi, Zhang Li-Xia, et al. J. Mol. Struc., 2005, 750: 174.
[6] WU Dan, XU Gui-ying(吴 丹，徐桂英). Acta Physico-Chimica Sinica(物理化学学报)，2006，22(2): 254.
[7] BI Shu-yun, DING Lan, SONG Da-qian, et al(毕淑云，丁 兰，宋大千, 等). Acta Chimica Sinica(化学学报)，2005，63(23): 2169.
[8] Lakowicz J R, Weber G. Biochemistry, 1973, 12: 4161.
[9] ZHANG Hai-rong, GUO Si-yuan, LI Lin, et al(张海容，郭祀远，李 琳, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2001，21(6): 829.
[10] Ross P D, Subramanian S. Biochemistry, 1981, 20: 3096.
[11] XIE Meng-xia, XU Xiao-yun, WANG Ying-dian, et al(谢孟峡，徐晓云，王英典, 等). Acta Chimica Sinica(化学学报)，2005，63(22): 2055.
[12] LIU Luo-sheng, WANG Xing-bo, ZHAO Quan-qin, et al(刘洛生，王兴波，赵全芹, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2006，26(6): 1130. |
| [1] |
LEI Hong-jun1, YANG Guang1, PAN Hong-wei1*, WANG Yi-fei1, YI Jun2, WANG Ke-ke2, WANG Guo-hao2, TONG Wen-bin1, SHI Li-li1. Influence of Hydrochemical Ions on Three-Dimensional Fluorescence
Spectrum of Dissolved Organic Matter in the Water Environment
and the Proposed Classification Pretreatment Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 134-140. |
| [2] |
GU Yi-lu1, 2，PEI Jing-cheng1, 2*，ZHANG Yu-hui1, 2，YIN Xi-yan1, 2，YU Min-da1, 2, LAI Xiao-jing1, 2. Gemological and Spectral Characterization of Yellowish Green Apatite From Mexico[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 181-187. |
| [3] |
HAN Xue1, 2, LIU Hai1, 2, LIU Jia-wei3, WU Ming-kai1, 2*. Rapid Identification of Inorganic Elements in Understory Soils in
Different Regions of Guizhou Province by X-Ray
Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 225-229. |
| [4] |
WANG Hong-jian1, YU Hai-ye1, GAO Shan-yun1, LI Jin-quan1, LIU Guo-hong1, YU Yue1, LI Xiao-kai1, ZHANG Lei1, ZHANG Xin1, LU Ri-feng2, SUI Yuan-yuan1*. A Model for Predicting Early Spot Disease of Maize Based on Fluorescence Spectral Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3710-3718. |
| [5] |
CHENG Hui-zhu1, 2, YANG Wan-qi1, 2, LI Fu-sheng1, 2*, MA Qian1, 2, ZHAO Yan-chun1, 2. Genetic Algorithm Optimized BP Neural Network for Quantitative
Analysis of Soil Heavy Metals in XRF[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3742-3746. |
| [6] |
SONG Yi-ming1, 2, SHEN Jian1, 2, LIU Chuan-yang1, 2, XIONG Qiu-ran1, 2, CHENG Cheng1, 2, CHAI Yi-di2, WANG Shi-feng2，WU Jing1, 2*. Fluorescence Quantum Yield and Fluorescence Lifetime of Indole, 3-Methylindole and L-Tryptophan[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3758-3762. |
| [7] |
YANG Ke-li1, 2, PENG Jiao-yu1, 2, DONG Ya-ping1, 2*, LIU Xin1, 2, LI Wu1, 3, LIU Hai-ning1, 3. Spectroscopic Characterization of Dissolved Organic Matter Isolated From Solar Pond[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3775-3780. |
| [8] |
LI Xiao-li1, WANG Yi-min2*, DENG Sai-wen2, WANG Yi-ya2, LI Song2, BAI Jin-feng1. Application of X-Ray Fluorescence Spectrometry in Geological and
Mineral Analysis for 60 Years[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 2989-2998. |
| [9] |
XUE Fang-jia, YU Jie*, YIN Hang, XIA Qi-yu, SHI Jie-gen, HOU Di-bo, HUANG Ping-jie, ZHANG Guang-xin. A Time Series Double Threshold Method for Pollution Events Detection in Drinking Water Using Three-Dimensional Fluorescence Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3081-3088. |
| [10] |
MA Qian1, 2, YANG Wan-qi1, 2, LI Fu-sheng1, 2*, CHENG Hui-zhu1, 2, ZHAO Yan-chun1, 2. Research on Classification of Heavy Metal Pb in Honeysuckle Based on XRF and Transfer Learning[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2729-2733. |
| [11] |
YANG Jing1, LI Li1, LIANG Jian-dan1, HUANG Shan1, SU Wei1, WEI Ya-shu2, WEI Liang1*, XIAO Qi1*. Study on the Interaction Mechanism Between Thiosemicarbazide Aryl Ruthenium Complexes and Human Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2761-2767. |
| [12] |
JIA Yu-ge1, YANG Ming-xing1, 2*, YOU Bo-ya1, YU Ke-ye1. Gemological and Spectroscopic Identification Characteristics of Frozen Jelly-Filled Turquoise and Its Raw Material[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2974-2982. |
| [13] |
YANG Xin1, 2, XIA Min1, 2, YE Yin1, 2*, WANG Jing1, 2. Spatiotemporal Distribution Characteristics of Dissolved Organic Matter Spectrum in the Agricultural Watershed of Dianbu River[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2983-2988. |
| [14] |
CHEN Wen-jing, XU Nuo, JIAO Zhao-hang, YOU Jia-hua, WANG He, QI Dong-li, FENG Yu*. Study on the Diagnosis of Breast Cancer by Fluorescence Spectrometry Based on Machine Learning[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2407-2412. |
| [15] |
ZHU Yan-ping1, CUI Chuan-jin1*, CHENG Peng-fei1, 2, PAN Jin-yan1, SU Hao1, 2, ZHANG Yi1. Measurement of Oil Pollutants by Three-Dimensional Fluorescence
Spectroscopy Combined With BP Neural Network and SWATLD[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2467-2475. |
|
|
|
|
