光谱学与光谱分析 |
|
|
|
|
|
Investigations on Colorectum Cancer Cell Lines and Nucleus by FTIR Spectroscopy |
ZHANG Chao1, SUN Xue-jun1*, LIU Dong1, LIU Wei3, ZHENG Jian-bao1, DU Jun-kai1, LING Xiao-feng4, ZHANG Yuan-fu2, XU Yi-zhuang2, WU Jin-guang2 |
1. First Hospital of Xi’an Jiaotong University, Xi’an 710061, China 2. College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China 3. College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China 4. Peking University Third Hospital, Beijing 100191,China |
|
|
Abstract The purpose of this study is to establish the cell and subcellular basis for the diagnosis of colorectal cancer by researching the FTIR( Fourier transform infrared spectra) of the normal tissue (30 cases) and cell line (SW 620), normal nuclei and malignant nucleus with Fourier transform infrared spectrum technology. The results of the peak position and the relative intensity obtained by statistical analysis indicate that the bands at 2 925, 1 240 and 1 085 cm-1 shifted toward higher wavenumber (p<0.05) in the FTIR spectra of cell lines (SW 620) and malignant nucleus. The 1 400 cm-1 band shifted toward lower wave number (p<0.05). The relative intensity ratios of I1 650/I1 460, I1 400/I1 460 and I1 240/I1 460 increased in the FTIR spectra of cell lines (SW 620) compared with normal tissue (p<0.05), but inversely, I1 740/I1 460 decreased significantly (p<0.01). The relative intensity ratios of I1 650/I1 460, I1 400/I1 460 and I1 240/I1 460 also increased in the FTIR spectra of the malignant nucleus. The above statistic differences are the basis for diagnosing colorectum cancer by FTIR spectroscopy.
|
Received: 2012-09-09
Accepted: 2013-01-24
|
|
Corresponding Authors:
SUN Xue-jun
E-mail: sunxy@mail.xjtu.edu.cn
|
|
[1] Kendall C, Isabelle M, Bazant-Hegemark F, et al. Analyst, 2009, 134(6): 1029. [2] Li Qingbo, Xu Zhi, Zhang Nengwei, et al. World J. Gastroenterol, 2005, 11(3): 327. [3] Zwielly A, Mordechai S, Sinielnikov I, et al. Midical Physics, 2010, 37(3): 1047. [4] YAO Hong-wei, LIU Ya-qi, FU Wei, et al(姚宏伟,刘亚奇,傅 卫,等). Chemical Journal of Chinese Universities(高等学校化学学报), 2011, 31(2): 297. [5] Garber K. Science, 2016, 312: 1158. [6] Yang Y, Sule-Suso J, Sockalingum G D, et al. Biopolymers, 2005, 78: 311. [7] Fujioka N, Morimoto Y, Arai T, et al. Cancer Detection & Prevention, 2004, 28: 32. [8] LING Xiao-feng, XU Yi-zhuang, WANG Li-xin, et al(凌晓锋, 徐怡庄, 王立新, 等). Chemical Journal of Chinese Universities(高等学校化学学报), 2007, 28(3): 445. [9] Dovbeshko G I, Chegel V I, Gridina N Y, et al. Biopolymer (Biospectroscopy), 2002, 67: 470. [10] Shetty G, Kedall C, Shepherd N, et al. British Journal of Cancer, 2006, 94: 1460. [11] Chen Liang, Hoi-Ying N Holman, Zhao Hao, et al. Analytical Chemistry, 2012, 84: 4118. |
[1] |
GUO Ya-fei1, CAO Qiang1, YE Lei-lei1, ZHANG Cheng-yuan1, KOU Ren-bo1, WANG Jun-mei1, GUO Mei1, 2*. Double Index Sequence Analysis of FTIR and Anti-Inflammatory Spectrum Effect Relationship of Rheum Tanguticum[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 188-196. |
[2] |
TIAN Ze-qi1, WANG Zhi-yong1, YAO Jian-guo1, GUO Xu1, LI Hong-dou1, GUO Wen-mu1, SHI Zhi-xiang2, ZHAO Cun-liang1, LIU Bang-jun1*. Quantitative FTIR Characterization of Chemical Structures of Highly Metamorphic Coals in a Magma Contact Zone[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2747-2754. |
[3] |
ZHANG Xiao-xu1, LIN Xiao-xian3, ZHANG Dan2, ZHANG Qi1, YIN Xue-feng2, YIN Jia-lu3, 4, ZHANG Wei-yue4, LI Yi-xuan1, WANG Dong-liang3, 4*, SUN Ya-nan1*. Study on the Analysis of the Relationship Between Functional Factors and Intestinal Flora in Freshly Stewed Bird's Nest Based on Fourier Transform Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2452-2457. |
[4] |
WANG Yu-hao1, 2, LIU Jian-guo1, 2, XU Liang2*, DENG Ya-song2, SHEN Xian-chun2, SUN Yong-feng2, XU Han-yang2. Application of Principal Component Analysis in Processing of Time-Resolved Infrared Spectra of Greenhouse Gases[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 2313-2318. |
[5] |
SU Ling1, 2, BU Ya-ping1, 2, LI Yuan-yuan2, WANG Qi1, 2*. Study on the Prediction Method of Pleurotus Ostreatus Protein and
Polysaccharide Content Based on Fourier Transform Infrared
Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(04): 1262-1267. |
[6] |
ZHOU Ao1, 2, YUE Zheng-bo1, 2, LIU A-zuan1, 2, GAO Yi-jun3, WANG Shao-ping3, CHUAI Xin3, DENG Rui1, WANG Jin1, 2*. Spectral Analysis of Extracellular Polymers During Iron Dissimilar
Reduction by Salt-Tolerant Shewanella Aquimarina[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(04): 1320-1328. |
[7] |
FENG Yu, ZHANG Yun-hong*. Rapid ATR-FTIR Principal Component Analysis of Commercial Milk[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(03): 838-841. |
[8] |
YUE Kong, LU Dong, SONG Xue-song. Influence of Thermal Modification on Poplar Strength Class by Fourier Infrared Spectroscopy Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(03): 848-853. |
[9] |
ZHANG Yan1, 2, WANG Hui-le1, LIU Zhong2, ZHAO Hui-fang1, YU Ying-ying1, LI Jing1, TONG Xin1. Spectral Analysis of Liquefaction Residue From Corn Stalk Polyhydric
Alcohols Liquefaction at Ambient Pressure[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(03): 911-916. |
[10] |
QIAO Lu1, LIU Rui-na1, ZHANG Rui1, ZHAO Bo-yu1, HAN Pan-pan1, 2, ZHOU Chun-ya1, 3, ZHANG Yu-qing1, 4, DONG Cheng-ming1*. Analysis of Spectral Characteristics of Soil Under Different Continuous Cropping of Rehmannia Glutinosa Based on Infrared Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(02): 541-548. |
[11] |
CHEN Yong1, 2, GUO Yun-zhu1, WANG Wei3*, WU Xiao-hong1, 2*, JIA Hong-wen4, WU Bin4. Clustering Analysis of FTIR Spectra Using Fuzzy K-Harmonic-Kohonen Clustering Network[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(01): 268-272. |
[12] |
HU Yun-you1, 2, XU Liang1*, XU Han-yang1, SHEN Xian-chun1, SUN Yong-feng1, XU Huan-yao1, 2, DENG Ya-song1, 2, LIU Jian-guo1, LIU Wen-qing1. Adaptive Matched Filter Detection for Leakage Gas Based on Multi-Frame Background[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(10): 3307-3313. |
[13] |
JING Jian-yuan, YUAN Liang, ZHANG Shui-qin, LI Yan-ting, ZHAO Bing-qiang*. Multispectral Structural Characterization of Humic Acid-Enhanced Urea[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(08): 2610-2615. |
[14] |
LI Shu-jie1, LIU Jie1, DENG Zi-ang1, OU Quan-hong1, SHI You-ming2, LIU Gang1*. Study of Germinated Rice Seeds by FTIR Spectroscopy Combined With Curve Fitting[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(06): 1832-1840. |
[15] |
ZHA Ling-ling1, 2, 3, WANG Wei2*, XIE Yu1, SHAN Chang-gong2, ZENG Xiang-yu2, SUN You-wen2, YIN Hao2, HU Qi-hou2. Observation of Variations of Ambient CO2 Using Portable FTIR
Spectrometer[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(04): 1036-1043. |
|
|
|
|