| 光谱学与光谱分析 |
|
|
|
|
|
| Effects of PEMF on Fluorescence Spectra of Rats Serum |
| ZHANG Ya-mei1, ZHOU Yan1, 2, PANG Xiao-feng1* |
1. School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China
2. Department of Radiation Medicine, Faculty of Preventive Medicine, Fourth Military Medical University, Xi’an 710032, China |
|
|
|
|
Abstract To investigate the relationship between the fluorescence spectra of serum and the brain injury effect, the alteration of fluorescence emission in serum was collected by fluorescence spectroscopy, the pathologic changes in the rat brain were obvious by histopathology after exposure to PEMF. Sprague-Dawley male rats were randomized into sham exposed and PEMFs exposed groups. After exposure to PEMF(3.5 ns rising time, 14 ns pulse width, and amplitude up to 200 kV/400 kV at 1 Hz repetitive rate) at 0.5, 1, 3, 6 and 12 h, the expression of S100B and the fluorescence spectra in serum were detected, the changes in brain morphology were observed. The results showed that the fluorescence intensity of 400 kV groups were higher than 200 kV groups at different time points after exposure to PEMF. It suggests that the extent of brain injury was associated with the pulse frequency. The trends of fluorescence spectra in serum coincide with the expression of S100B and pathologic changes. It shows that fluorescence spectroscopy could apply to analysis of the effect of brain injury after exposure to PEMF.
|
|
Received: 2011-08-23
Accepted: 2012-04-04
|
|
|
|
Corresponding Authors:
PANG Xiao-feng
E-mail: pangxf2006@yahoo.com.cn
|
|
[1] Del Vecchio G, Giuliani A, Fernandez M, et al. Bioelectromagnetics, 2009, 30(7): 564.
[2] Nittby H, Grafstrom G, Tian D P, et al. Bioelectromagnetics, 2008, 29(3): 219.
[3] Keetley V, Wood A W, Spong J, et al. Neuropsychologia, 2006, 44(10): 1843.
[4] Maier R. Biomed. Tech., 2001, 46(1-2): 18.
[5] Ding G R, Li K C, Wang X W, et al. Biomed. Environ. Sci., 2009, 22(3): 265.
[6] Zhang Y M, Zhou Y, Pang X F, et al. Biomed. Environ. Sci., 2011, 24.
[7] Sparidans R W, Langendijk P, Boers E, et al. J. Chromatogr B Analyt Technol. Biomed. Life Sci., 2010, 878(23): 2168.
[8] Rothermundt M, Peters M, Prehn J H, et al. Microsc. Res. Tech., 2003, 60(6): 614.
[9] Goncalves C A, Leite M C, Nardin P. Clin. Biochem., 2008, 41(10-11): 755.
[10] Heizmann C W. Neurochem. Res., 1999, 24(9): 1097.
[11] Donato R. Biochim. Biophys. Acta, 1999, 1450(3): 191.
[12] Bohmer A E, Oses J P, Schmidt A P, et al. Neurosurgery, 2011, 68(6): 1624.
[13] Vos P E, Jacobs B, Andriessen T M, et al. Neurology 2010, 75(20): 1786.
[14] Lakowicz J R. Principles of Fluorescence Spectroscopy. New York: Plenum Press, 2006. 255.
[15] Gorka J, Zuwala J J, Pazgan S M, et al. Przegl Epidemiol, 2008, 62(2): 393.
[16] Dickerson B D, Geist B L, Spillman W B, et al. Appl. Opt., 2007, 46(33): 8080. |
| [1] |
LEI Hong-jun1, YANG Guang1, PAN Hong-wei1*, WANG Yi-fei1, YI Jun2, WANG Ke-ke2, WANG Guo-hao2, TONG Wen-bin1, SHI Li-li1. Influence of Hydrochemical Ions on Three-Dimensional Fluorescence
Spectrum of Dissolved Organic Matter in the Water Environment
and the Proposed Classification Pretreatment Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 134-140. |
| [2] |
GU Yi-lu1, 2，PEI Jing-cheng1, 2*，ZHANG Yu-hui1, 2，YIN Xi-yan1, 2，YU Min-da1, 2, LAI Xiao-jing1, 2. Gemological and Spectral Characterization of Yellowish Green Apatite From Mexico[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 181-187. |
| [3] |
HAN Xue1, 2, LIU Hai1, 2, LIU Jia-wei3, WU Ming-kai1, 2*. Rapid Identification of Inorganic Elements in Understory Soils in
Different Regions of Guizhou Province by X-Ray
Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 225-229. |
| [4] |
WANG Hong-jian1, YU Hai-ye1, GAO Shan-yun1, LI Jin-quan1, LIU Guo-hong1, YU Yue1, LI Xiao-kai1, ZHANG Lei1, ZHANG Xin1, LU Ri-feng2, SUI Yuan-yuan1*. A Model for Predicting Early Spot Disease of Maize Based on Fluorescence Spectral Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3710-3718. |
| [5] |
CHENG Hui-zhu1, 2, YANG Wan-qi1, 2, LI Fu-sheng1, 2*, MA Qian1, 2, ZHAO Yan-chun1, 2. Genetic Algorithm Optimized BP Neural Network for Quantitative
Analysis of Soil Heavy Metals in XRF[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3742-3746. |
| [6] |
SONG Yi-ming1, 2, SHEN Jian1, 2, LIU Chuan-yang1, 2, XIONG Qiu-ran1, 2, CHENG Cheng1, 2, CHAI Yi-di2, WANG Shi-feng2，WU Jing1, 2*. Fluorescence Quantum Yield and Fluorescence Lifetime of Indole, 3-Methylindole and L-Tryptophan[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3758-3762. |
| [7] |
YANG Ke-li1, 2, PENG Jiao-yu1, 2, DONG Ya-ping1, 2*, LIU Xin1, 2, LI Wu1, 3, LIU Hai-ning1, 3. Spectroscopic Characterization of Dissolved Organic Matter Isolated From Solar Pond[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3775-3780. |
| [8] |
LI Xiao-li1, WANG Yi-min2*, DENG Sai-wen2, WANG Yi-ya2, LI Song2, BAI Jin-feng1. Application of X-Ray Fluorescence Spectrometry in Geological and
Mineral Analysis for 60 Years[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 2989-2998. |
| [9] |
XUE Fang-jia, YU Jie*, YIN Hang, XIA Qi-yu, SHI Jie-gen, HOU Di-bo, HUANG Ping-jie, ZHANG Guang-xin. A Time Series Double Threshold Method for Pollution Events Detection in Drinking Water Using Three-Dimensional Fluorescence Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3081-3088. |
| [10] |
MA Qian1, 2, YANG Wan-qi1, 2, LI Fu-sheng1, 2*, CHENG Hui-zhu1, 2, ZHAO Yan-chun1, 2. Research on Classification of Heavy Metal Pb in Honeysuckle Based on XRF and Transfer Learning[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2729-2733. |
| [11] |
YANG Jing1, LI Li1, LIANG Jian-dan1, HUANG Shan1, SU Wei1, WEI Ya-shu2, WEI Liang1*, XIAO Qi1*. Study on the Interaction Mechanism Between Thiosemicarbazide Aryl Ruthenium Complexes and Human Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2761-2767. |
| [12] |
JIA Yu-ge1, YANG Ming-xing1, 2*, YOU Bo-ya1, YU Ke-ye1. Gemological and Spectroscopic Identification Characteristics of Frozen Jelly-Filled Turquoise and Its Raw Material[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2974-2982. |
| [13] |
YANG Xin1, 2, XIA Min1, 2, YE Yin1, 2*, WANG Jing1, 2. Spatiotemporal Distribution Characteristics of Dissolved Organic Matter Spectrum in the Agricultural Watershed of Dianbu River[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2983-2988. |
| [14] |
CHEN Wen-jing, XU Nuo, JIAO Zhao-hang, YOU Jia-hua, WANG He, QI Dong-li, FENG Yu*. Study on the Diagnosis of Breast Cancer by Fluorescence Spectrometry Based on Machine Learning[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2407-2412. |
| [15] |
ZHU Yan-ping1, CUI Chuan-jin1*, CHENG Peng-fei1, 2, PAN Jin-yan1, SU Hao1, 2, ZHANG Yi1. Measurement of Oil Pollutants by Three-Dimensional Fluorescence
Spectroscopy Combined With BP Neural Network and SWATLD[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2467-2475. |
|
|
|
|
