| 光谱学与光谱分析 |
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| Biosynthesis of CdS Quantum Dots in Saccharomyces Cerevisiae and Spectroscopic Characterization |
| HUANG Huai-qing, HE Ming-xin, WANG Wen-xing, LIU Jin-ling, MI Cong-cong, XU Shu-kun* |
| Department of Chemistry, Northeastern University, Shenyang 110819, China |
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Abstract In the present work, CdS quantum dots (QDs) were successfully biosynthesized at room temperature by using saccharomyces cerevisiae yeast as a carrier. Fluorescence emission spectra, ultraviolet-visible (UV/Vis) absorption spectra, and inverted fluorescence microscope images confirmed that saccharomyces cerevisiae can be used to biosynthesize CdS QDs. The as-prepared CdS QDs show the fluorescence emission peak at 443 nm and emit blue-green fluorescence under UV light (with excitation at 365 nm). Transmission electron microscopy (TEM) was applied to characterize the as-prepared CdS QDs and the TEM results showed that the as-prepared CdS QDs had the structure of hexagonal wurtzite. Fluorescence emission spectrum and UV/Vis absorption spectrum were used as the performance indicatiors to study the effects of saccharomyces cerevisiae yeast incubation times, reactant Cd2+ concentrations and reaction times on CdS QDs synthesis. Saccharomyces cerevisiae yeast grown in early stable phase can get the highest fluorescence intensity of CdS QDs when they were co-cultured with 0.5 mmol·L-1 of Cd2+ with 24 h incubation time. Furthermore, much more CdS QDs can be obtained by changing the culture medium during the synthesis process.
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Received: 2011-09-29
Accepted: 2011-12-06
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Corresponding Authors:
XU Shu-kun
E-mail: xushukun46@126.com
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