光谱学与光谱分析 |
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Study on the Microstructure of Fluorescent Labelling Ghee Microcapsules by Tomoscan Imaging |
SHI Yan,ZHENG Wei-wan,ZOU Jin,ZHANG Xue-chun, LIU Fan |
State Key Laboratory of Food Science and Technology and Department of Food Science and Engineering, Nanchang University,Nanchang 330047, China |
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Abstract In the present paper, fluorescein isothiocyanate was chosen as a fluorescence probe to mark casein protein in alkaline conditions. The interaction of the casein protein marked or not marked and fluorescein isothiocyanate was preliminarily discussed by the spectrum changes of UV-absorption and fluorescence spectrometry. Fluorescent marker was separated from SephadexG-50 chromatography column. With it as an emulsifier, the fluorescently-labeled ghee microcapsules were prepared by spray drying. And using laser scanning confocal microscope by tomoscan imaging to detect the microstructure of ghee microcapsules with the excitation of 488 nm argon-ion laser, the results showed that the casein protein assembled in the membrane surface of oil-water interface and microcapsules. The ghee microcapsules had two forms, namely mononuclear and multinuclear. The microcapsule was spherical. Its surface was smooth with no crack and no hollow. Its wall surface was intact and wall structure was relatively dense. The particle size showed obvious difference. Small particles attached to large particles, forming partial agglomerating powders to contribute to enhancing the solubility of microcapsules. These prove that the ghee microcapsule is an ideal microcapsule product.
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Received: 2010-05-02
Accepted: 2010-08-06
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Corresponding Authors:
SHI Yan
E-mail: kwei@ncu.edu.cn
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[1] Maria Inês Teixeira, Leonardo R Andrade, Marcos Farina,et al. Materials Science and Engineering, 2004, 24(5): 653. [2] Drusch S, Berg S. Food Chemistry, 2008, 109(1): 17. [3] Biju S S, Saisivam S, Maria Gerald Rajan N S, et al. European Journal of Pharmaceutics and Biopharmaceutics, 2004, 58(1): 61. [4] Zhang Fu, Wu Qi, Liu Li-jun, et al. International Journal of Pharmaceutics, 2008, 357(1): 22. [5] Ehab Taqieddin, Mansoor Amiji. Biomaterials, 2004, 25(10): 1937. [6] Ma J, Qi W T, Yang L N, et al. Journal of Microbiological Methods, 2007, 68(1): 172. [7] Wang J P, Zhao X P, Guo H L. Optical Materials, 2008, 30(8): 1268. [8] Guo H X, Heinmki J, Yliruusi J. International Journal of Pharmaceutics, 1999, 186(2): 99. [9] Lamprecht A, Sch?fer U F, Lehr C-M. European Journal of Pharmaceutics and Ciopharmaceutics, 2000, 49(1): 1. [10] Halbhuber Karl-Jürgen, Knig Karsten. Annals of Anatomy-Anatomischer Anzeiger, 2003, 185(1): 1. [11] Roldán J C, Chang E, Kelantan M, et al. Journal of Cranio-Maxillofacial Surgery, 2010. 1. [12] Chen Pei, Yu Long, Simon George, et al. Journal of Cereal Science, 2009, 50(2): 241. [13] DAI Guo-liang, DAI Lian-hua, YU Yong, et al(戴国亮,代连花,于 泳,等). Acta Chimica Sinica(化学学报), 2005, 63(7): 559. [14] Johan Benesch, Graham Hungerford, Klaus Suhling, et al. Journal of Colloid and Interface Science, 2007, 312(2): 193. [15] LIANG Zhi-qi(梁治齐). Microcapsule Technology and Application(微胶囊技术及其应用). Beijing: China Light Industry Press(北京: 中国轻工业出版社),1999. 113. [16] ZHU Xuan, HUANG Hui-ming, XU Shi-ying(朱 选,黄慧敏,许时婴). Journal of Chinese Institute of Food Science and Technology(中国食品学报), 2003, 3(1): 32.
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