| 光谱学与光谱分析 |
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| The Interaction of Berberine and Human Serum Albumin |
| ZHAO Chang-chun1, ZHENG Wei-fa2, LI Meng-qiu2 |
1. Department of Chemistry, Xuzhou Normal University, Xuzhou 221009，China
2. Key Lab of Biotechnology on Medicinal Plants of Jiangsu Province, Xuzhou 221009, China |
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Abstract The interaction of berberine and Human Serum Albumin(HSA) has been studied by fluorescence and UV-Vis absorption spectroscopy. With fluorescence quenching method, the binding constant K was found to be 1.168×105 L·mol-1 and the number of binding site n was 5.26. The binding distance(R=3.44 nm) and energy transfer efficiency (E=0.303) were also obtained according to Frster’s non-radiation energy transfer theory. The energy transfer path between tyrosine residual and tryptophan residual was cut by berberine in the site of HSA and weak fluorescence berberine-HSA complex was also observed.
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Received: 2002-09-26
Accepted: 2003-02-15
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Corresponding Authors:
ZHAO Chang-chun
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| Cite this article: |
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ZHAO Chang-chun,ZHENG Wei-fa,LI Meng-qiu. The Interaction of Berberine and Human Serum Albumin [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(01): 111-113.
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| URL: |
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http://www.gpxygpfx.com/EN/Y2004/V24/I01/111 |
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