| 光谱学与光谱分析 |
|
|
|
|
|
| Catalysis-fluorescence Determination of Trace Nitrite Based on the Oxidation of Acridine Orange by Potassium Bromate |
| BAO Suo-yan1 ，LIU Wei-jie1, 2，HU Han-fang1 |
1. College of Chemistry and Environment Science, Hebei University，Baoding 071002，China
2. Department of Chemistry，Handan Normal College，Handan 056004，China |
|
|
|
|
Abstract Acridine orange can be oxidized with discoloring and fluorescence quenching by potassium bromate in phosphoric acid medium, but the reaction rate is very low. The presence of trace nitrite can catalyze this reaction to improve the sensitivity of it apparently and to make the net fluorescence value of the system decrease greatly. Based on the above investigation, a new catalysis-fluorescence determination method of trace nitrite has been proposed. The method described is very sensitive and selective. The catalysis reaction is under the conditions of λeｘ/λem＝446/505 nm, 55 ℃ and 10 min for the reaction time. The method permits the existence of more than 20 common foreign ions. There isn’t interference by the same quantity of Fe3+, Br- and I-. The detection limit is 0.012 ng·mL-1. The linear range of determination is 0.05-5 ng·mL-1. The method has been used for the determination of trace nitrite in water and soil samples with satisfactory results.
|
|
Received: 2002-05-08
Accepted: 2002-09-28
|
|
|
|
Corresponding Authors:
BAO Suo-yan
|
|
| Cite this article: |
|
BAO Suo-yan,LIU Wei-jie,HU Han-fang. Catalysis-fluorescence Determination of Trace Nitrite Based on the Oxidation of Acridine Orange by Potassium Bromate [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(03): 342-344.
|
|
|
|
| URL: |
|
http://www.gpxygpfx.com/EN/Y2004/V24/I03/342 |
[1] LIU Xi-dong，LIU Shao-pu(刘希东，刘绍璞). Chinese J. of Anal. Chem.(分析化学), 1998, 26(4): 491.
[2] BAO Suo-yan et al(鲍所言等). Chinese J. of Anal. Chem.(分析化学)，1995, 23(2): 191.
[3] YAN Jin et al(严 进等). Physical Testing and Chemical Analysis, Part B: Chemical Analysis(理化检验-化学分册)，2000, 36(11): 504.
[4] ZHENG Zhao-sheng et al(郑肇生等). Chinese J. of Anal. Chem.(分析化学), 1992, 20(1): 91.
[5] CHEN Lan-hua，CHEN De-quan(陈兰化，陈德权). Chinese J. of Anal. Chem.(分析化学), 1996, 24(7): 790.
|
| [1] |
HOU Ming1,FENG Li-e1,JIANG Zhi-liang1, 2. Study on the Energy Transfer Fluorescence Quenching Reaction of Acridine Orange-4-(2-Pyridylazo) Resorcinol-V(Ⅴ)System and Analytical Application [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(02): 476-478. |
| [2] |
LIN Shao-yu1, 2,GAO Wen-hua1*,LIU Xiao-xuan1,CHEN Yao-wen1 . Determination of Trace Chromium(Ⅵ) by Catalytic Spectrofluorimetry Using Pyronine Y-H2O2-Cr(Ⅵ) System [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(01): 113-115. |
| [3] |
SUN Wei, DING Ya-qin, TENG Wen-hui, JIAO Kui. Spectrophotometric Study on the Interaction of Acridine Orange and Heparin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(01): 116-119. |
| [4] |
LIU Bao-sheng, SUN Hua-yang, LIU Zhi-chao, GUO Yuan . Study on the Mechanism of Energy Transfer between Acridine Orange and Rhodamine 6G in Micelles of Dodecyl Benzene Sodium Sulfonate [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(11): 2093-2096. |
| [5] |
SHI Yan, ZHENG Wei-wan, WU Xiao-xing, ZHOU De-hong . The Interaction of Acridine Orange with Anionic Surfactant and Its Application to the Determination of Protein[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(09): 1653-1655. |
| [6] |
LIU Bao-sheng, GAO Jing, LIU Zhi-chao, YU Li-na, YANG Da-cheng, YANG Geng-liang. Fluorescence Resonance Energy Transfer Quenching Method for Determination of Arsenic with Acridine Orange-Rhodamine B[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(02): 306-308. |
| [7] |
ZI Yan-qin, LU Hao-miao. Study on Determination of Trace Nitrite and Reaction Mechanism by Two-Wavelength Negative Absorption-Catalytic Spectrophotometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2006, 26(01): 137-139. |
| [8] |
LIU Bao-sheng, GAO Jing, YANG Geng-liang. Determination of Vitamin B12 Concentration by Fluorescence Quenching with Acridine Orange-Rhodamine 6G Energy Transfer System[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(07): 1080-1082. |
| [9] |
WAN Xin-jun1, LIU Jin-shui2, WANG Lun2*. Determination of Deoxyribonucleic Acid with Acridine Orange by Resonance Light Scattering Technique[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(05): 754-756. |
|
|
|
|
