| 光谱学与光谱分析 |
|
|
|
|
|
| Study on the Fluorescence Spectra of LaL3 with DNA |
| WANG Xing-min1，SONG Yu-min2*，TENG Xiu-lan2, GENG Zhi-yuan2, YANG Pei-ju2, YANG Mei-ling2 |
1. Basic Sciences Department, Gansu Agriculture University, Lanzou 730070，China
2.Collece of Chemistry and Chemical Engineering, Northwest Normal University, Lanzou 730070，China |
|
|
|
|
Abstract A fluorescence method is presented for the determination of DNA.The method is based on the interaction of LaL3 [L=morin(2′, 3, 4′, 5, 7-Pentahydroxyflavone), 2′-OH group deprotonated] with DNA in NH3·H2O-NH4Cl buffer of pH=8.0, with λex at 387 nm, and λem at 535 nm. Enhanced fluorescence was observed for LaL3 in the presence of DNA and in the presence of buffer solution of 10%(φ) and ethanol of 10%(φ). The linear range of determination was between 0 and 15 μg·mL-11 for DNA.The method proved to be simple, easy and sensitive for the determination of DNA. The mechanism of enhancing and the reasons for the effects of acid were also discussed.
|
|
Received: 2002-06-28
Accepted: 2002-11-08
|
|
|
|
Corresponding Authors:
SONG Yu-min
|
|
| Cite this article: |
|
WANG Xing-min,SONG Yu-min,TENG Xiu-lan, et al. Study on the Fluorescence Spectra of LaL3 with DNA [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(03): 339-341.
|
|
|
|
| URL: |
|
http://www.gpxygpfx.com/EN/Y2004/V24/I03/339 |
[1] ZHOU Xiu-fang，ZHENG Rong-liang(周秀芳，郑荣梁). Journal of Lanzhou University(兰州大学学报(自然科学版))，1991，27(3)：101.
[2] HUANG Cheng-zhi，LI Ｋe-an，DONG Shen-yang(黄承志，李克安，童沈阳). Chinese Journal of Analytical Chemistry(分析化学)，1997，25(7)：759.
[3] HUANG Cheng-zhi，LI Ｋe-an，DONG Shen-yang(黄承志，李克安，童沈阳). Chinese Journal of Analytical Chemistry(分析化学)，1997，25(9)：1052.
[4] ZHANG Qi, WANG Liu-fang，LIU Xiang. Transition Met. Chem.(过渡金属化学), 1996, 21: 23.
[5] GONG Guo-quan, ZONG Zhi-Xin，SONG Yu-min. Spectrochimica Acta，part A, 1999, 55: 1903.
[6] LIAO Jian-pei，LIU Guo-dong，HUANG Shan-sheng(廖见培，刘国东，黄杉生). Journal of Instrumental Analysis(分析测试学报)，2001，20(3)：5.
[7] FANG Guang-rong，SONG Gong-wu，LI Ying(方光荣，宋功武，李 英). Journal of Instrumental Analysis(分析测试学报)，2000，19 (2)：45.
[8] YANG Pin, YANG Bin-sheng(杨 频，杨斌盛). J. Conduction to A Method of Ion Probe(离子探针方法导论). Beijing: Science Press(北京：科学出版社)，1994. 159，160.
|
| [1] |
LÜ Jia-nan, LI Jun-sheng*, HUANG Guo-xia, YAN Liu-juan, MA Ji. Spectroscopic Analysis on the Interaction of Chrysene With Herring Sperm DNA and Its Influence Factors[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(01): 210-214. |
| [2] |
CHEN Yi-xue1, NIU Shan-shan2, LI Hong-ping2, YU Fei1, WU Yong-jun1, LIU Li-e1*. Determination of DNA Methyltransferases 1 by a Method of Fluorescence Immunoassay Based on Magnetic Particles[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(01): 162-167. |
| [3] |
KANG Xiao-yan1,2, ZHANG Ai-wu1,2*. A Novel Method for High-Order Residual Quantization-Based Spectral Binary Coding[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(10): 3013-3020. |
| [4] |
PENG Chen-jia1, WANG Ke-hua1*, LU Wei-xue1, GUAN Chun-qian1, HU Ya-jing1, MA Shuang1, ZHU Ming-chang2, GAO En-jun2. Synthesis, Crystal Structure and DNA/BSA Interaction of a Ni(Ⅱ) Coordination Compound[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(02): 559-564. |
| [5] |
QIU Lu1,2, XIE Mei-hua1,2, ZHAO Guo-yi1. Fourier Transform Infrared (FTIR) Spectroscopy of Genomic DNA from Forty-One Camellia Varieties[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(01): 325-332. |
| [6] |
LU Cai1, YU Hui2, XU Fei1*, GU Wei1,3, WU Qi-nan1,3,4, CHEN Jun1,3. Study of Anti-Tumor Mechanism of Alisol Acetates Based on p53DNA[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(12): 3683-3691. |
| [7] |
Belal H M Hussein1,2*, Mostafa A Gouda1,3, Omer Sakin1, Abdulla Faluji4, Mohamed Gomaa5, Hassan A Azab2, Walid Fathalla6, Sherin Arabi7, Sawsan Mosa8. DNA Binding and Antitumor Activity of a New Fluorescent Probe Eu(Ⅲ)-Complex Containing 4-Methyl-Coumarin Moiety[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(11): 3611-3621. |
| [8] |
Jong-Moon Kim1, Myung Duk Jang2, JIN Biao3*, Yoon Jung Jang4*. Comparison of Enhancement Effect of DNA-Mediated Energy Transfer by Divalent Cations: Mg2+, Ca2+, Mn2+, Co2+, and Ni2+[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1970-1974. |
| [9] |
LIU Ye1, ZHAO Wei-wei1, LI Zong-xiao1*, CHENG Hua-lei1, HE Huan2. Study on the Interaction of Aconitine and Armyworm DNA by UV Spectroscopy and ITC Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(03): 851-856. |
| [10] |
GONG Hong-ling1, LIU Yan-hui2*, TANG Yan-lin2*, HU Lin2. Effects of Temperature on DNA Condensation Detected with Temperature-Changed Ultraviolet Spectrum Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(06): 1831-1837. |
| [11] |
WANG Jia-hua, WU Zheng, WU Qiong-shui*, ZENG Li-bo* . The Study on Combined Screening Method for TBS and Cervical Cancer Cell’s DNA Quantitative Analysis Based on Multi-Spectral Imaging Technology[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(02): 545-550. |
| [12] |
WANG Meng. Synthesis of LaPO4∶Ce, Tb Fluorescent Nanopowders and Their Applications in Nondestructive Development of Latent Fingerprints[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(05): 1412-1417. |
| [13] |
WU Zheng, ZENG Li-bo*, WU Qiong-shui* . Study of Cervical Exfoliated Cell’s DNA Quantitative Analysis Based on Multi-Spectral Imaging Technology[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(02): 496-501. |
| [14] |
XIE Li-juan1, CHEN Zhuo2 . Pseudo-Furocoumarin: Synthesis, DNA-Binding Behavior and Cytotoxicity[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(11): 3051-3055. |
| [15] |
ZHAI Qiu-ge, GUO Peng, ZHOU Lin, LIU Yan-ming* . Sensitive Determination of Bi3+ by Spectrofluorimetry Based on Graphene Oxide-Methylene Blue System [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(08): 2152-2156. |
|
|
|
|
