| 光谱学与光谱分析 |
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| Study of Rare Earth Bifunctional Chelate BCPDA-Eu3+ Labeling Protein |
| PAN Li-hua1, AN Jin-long1, XIE Wen-bing1, GUAN Ming2 |
1． National Analytical Research Center of Electrochemistry and Spectroscopy, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
2. Jilin Universitｙ，Chanｇchｕn 130031, China |
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Abstract The authors labeled bovine serum albumin (BSA) with a new europium chelator 4,7-bis(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid (BCPDA) which was synthesized by solid phase time-resolved fluorescence immunoassay (TRFIA) technology. The process of BCPDA-labeling-BSA reaction was studied. Using Coomassie brilliant blue method, concentrations of BSA and BCPDA were determined in BCPDA-BSA labeled sample. The labeling reaction condition was studied. Labeling ratio and protein labeling recovery were calculated. BCPDA-BSA-Eu3+ complex has a very large Stokes shift (270 nm) and can emit very strong fluorescence band at 611.2 nm which is very narrow (10 nm). BCPDA-BSA-Eu3+ complex exhibits very long fluorescence lifetimes by the experiment demonstration. Also, BCPDA and protein-BCPDA-Eu3+ complex is relatively stable.
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Received: 2002-09-26
Accepted: 2003-02-16
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Corresponding Authors:
PAN Li-hua
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| Cite this article: |
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PAN Li-hua,AN Jin-long,XIE Wen-bing, et al. Study of Rare Earth Bifunctional Chelate BCPDA-Eu3+ Labeling Protein[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(07): 795-798.
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| URL: |
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http://www.gpxygpfx.com/EN/Y2004/V24/I07/795 |
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