光谱学与光谱分析 |
|
|
|
|
|
Study on Determination of Bovine Serum Albumin Using o-Hydroxyphenylfluorone Reagent with Fluorescence Spectrophotometry |
HUANG Ying-ping1,GU Yan2,FANG Yan-fen1,LIU Yu-liang1,ZHU Sheng-ji1,LOU Guang-fu1 |
1.China Three Gorges University Alan G MacDiarmid Institute for Renewable Energy, Yichang 443002, China 2.College of Mechanical and Material Engineering, China Three Gorges University, Yichang 443002, China |
|
|
Abstract The reaction conditions for the determination of bovine serum albumin (BSA) using o-hydroxyphenylfluorone (o-HPF) as fluorescence probe reagent were studied.In the B-R medium at pH 7.90, o-HPF could react with BSA, producing a stable complex compound which resulted in fluorescent quenching of this binary system.The quantitative assay was carried out based on the relation between the degree of fluorescent quenching and the additional amount of BSA.The method has advantages of good selectivity, stability and simplicity.The linear range of o-HPF fluorescence spectrophotometric method was 1.32-18.54 μg· mL-1.Moreover, the mechanism of the reaction BSA and o-HPF was discussed, and the quenching constant, and the thermodynamics constant of the fluorescence effects were calculated.The results showed that the nonconvalent binding forces were the binding force between o-HPF and BSA, and the quenching of BSA to o-HPF was probably a single static quenching process.
|
Received: 2007-02-06
Accepted: 2007-05-18
|
|
Corresponding Authors:
HUANG Ying-ping
E-mail: chem_ctgu@126.com
|
|
[1] Uirich Kragh-Hansen.The American Society for Pharmacology and Experimental Therapeutics, 1981, 33 (1):17. [2] Lin L, Tong S Y.Anal.Biochem., 1996, 233:151. [3] Saito Y A.Anal.Chim.Acta, 1985, 178(2):337. [4] HUANG Ying-ping, ZHANG Hua-shan(黄应平, 张华山).Chin.J.Anal.Chem.(分析化学), 2000, 28(2):164. [5] HUANG Ying-ping, ZHANG Hua-shan(黄应平, 张华山).Chin.J.Anal.Chem.(分析化学),2003,31(7):894. [6] Guo Z X, Shen H X.Analyst, 1999, 124(7):1093. [7] WEI Qin, DU Bin(魏 琴, 杜 斌).Chin.J.Anal.Chem.(分析化学), 2004, 32(3):370. [8] HUANG Ying-ping, ZHANG Hua-shan(黄应平, 张华山).Metallurgical Analysis(冶金分析), 2001, 21(2):34. [9] ZHANG Huan, WANG Xing-ming, WANG Qing-cheng, et al(张 欢, 王兴明, 王清成, 等).Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2006, 26(8):1508. [10] YI Ping-gui, LIU Jun-feng, SHANG Zhi-cai, et al(易平贵, 刘俊峰, 商志才, 等).Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2001, 21(6):826. |
[1] |
LI Jin-zhi1, LIU Chang-jin1, 4*, SHE Zhi-yu2, ZHOU Biao2, XIE Zhi-yong2, ZHANG Jun-bing3, JIANG Shen-hua2, 4*. Antiglycation Activity on LDL of Clove Essential Oil and the Interaction of Its Most Abundant Component—Eugenol With Bovine Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(01): 324-332. |
[2] |
WANG Jun1, WANG Zhou-li2, CHENG Jing-jing1. Interaction Between Tartrazine and Bovine Serum Albumin Using Multispectral Method and Molecular Docking[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(03): 904-909. |
[3] |
WANG Xiao-xia1,3*, WU Hao1, NIE Zhi-hua2, MA Li-tong1,3*, CUI Jin-long1, SAI Hua-zheng1, CHENG Jian-guo1. Study on the Interaction Between Fulvic Acid and Bovine Serum Albumin by Multispectral and Molecular Docking[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(09): 2904-2910. |
[4] |
ZHANG Chuan-ying1, PENG Xin1*, RAO Heng-jun2, QI Wei2, SU Rong-xin2, HE Zhi-min2. Spectroscopic Studies on the Interaction Between Salvianolic Acid B and Bovine Serum Albumin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(06): 1701-1707. |
[5] |
TUO Xun1, SONG Ji-min1, FU Hao2, LÜ Xiao-lan1*. Study on Interaction Between Hexabromocyclododecane and Bovine Serum Albumin by Spectroscopy and Computer Simulation Methods[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(05): 1487-1492. |
[6] |
LI Xue1, WANG Li1, LIU Guang-xian1*, TU Zong-cai2. Effect of Urea on Glycosylation of BSA Based on Spectral Techniques[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(02): 478-483. |
[7] |
LI Xing-xing, ZHANG Xiang, HUANG Xue-song*. Interaction Between Three Sulfur-Containing Amino Acids in Garlic and Bovine Serum Albumin Determined by Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(11): 3483-3488. |
[8] |
HUANG Fang1, LIU Ming-xue1, 2*, XIONG Jie1, CHEN Lü-qi1, GAO Zhu-xin1, CHEN Hui-ming1, WANG Dan-ni1. Effect of Far-Infrared Ceramic Powder on the Interaction Between Essential Oil and BSA[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(08): 2358-2365. |
[9] |
WANG Xiao-xia1*, NIE Zhi-hua2, MA Li-tong1, CUI Jin-long1, SAI Hua-zheng1,ZHAO Wen-yuan1. Study on the Interaction Between Minocycline and Bovine Serum Albumin by Multi Spectral Method and Molecular Docking Simulation[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(05): 1503-1508. |
[10] |
HUANG Chao-bo3, XU Han3, YANG Ming-guan3, LI Zhen-jing1, 3, YANG Hua1, 3, WANG Chang-lu1, 2, 3, ZHOU Qing-li1, 2, 3*. The Interaction between Rubropunctamine and Bovine Serum Albumin Using Spectrometry and Molecular Docking[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(10): 3102-3108. |
[11] |
ZHANG Qiu-lan1,2, XIE Li-xin3, YANG Lin-hui3, TUO Xun2, NI Yong-nian1,2*. Studies on the Interaction between Leucomalachite Green with Bovine Serum Albumin by MCR-ALS and Molecular Docking[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(03): 851-856. |
[12] |
TAN Hong-ying. Investigation of Bovine Serum Albumin at Different Temperatures by Terahertz Time-Domain Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(11): 3374-3378. |
[13] |
LUO Dan, ZHOU Guang-ming*, ZHANG Lu-tao, ZHANG Cai-hong. Analysis of Indomethacin by Surface Enhanced Raman Spectroscopy Based on L-Cys@Ag[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(10): 3112-3116. |
[14] |
WANG Xiao-xia1, NIE Zhi-hua2, LI Song-bo1, MA Li-tong1, LIU Jin-yan1, WANG Zheng-de1, YAN Hui1. Study on the Interaction between Tetracycline Hydrochloride and Bovine Serum Albumin by Multispectral and Molecular Docking[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(08): 2468-2476. |
[15] |
GONG Ai-qin1*, JIN Dang-qin1, ZHU Xia-shi2. A Fluorescence Spectroscopic Study of the Interaction between Trelagliptin Succinate and Bovin Serum Albumine and Its Analytical Application[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(01): 157-160. |
|
|
|
|