| 光谱学与光谱分析 |
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| A Novel Light-Emitting Diode-Induced Fluorescence Detector for Micro-Scale Flow Analysis System |
| YANG Bing-cheng,TAN Feng,GUAN Ya-feng* |
| Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116012, China |
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Abstract A compact light-emitting diode-induced fluorescence detector based on collinear scheme is described. The detector was assembled by all-solid-state optical-electronic components and easily coupled with micro-scale flow analysis system by on-column detection mode. Fluorescein isothiocyanate (FITC) and FITC-labeled amino acids were used as test analytes to evaluate the detector performance by capillary electrophoresis and flow injection analysis techniques. The concentration limit of detection was 10-8 mol·L-1 for FITC-labeled phenylalanine at a signal-to-noise ratio (S/N) of 3 (on-column, 0.05 mm i.d.). The system exhibited a good linear response in the range of 1×10-7-2×10-5 mol·L-1 (r2 =0.999). The relative standard deviation (RSD) of 6 repetitive injections on peak heights is less than 3%.
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Received: 2004-07-06
Accepted: 2004-11-08
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Corresponding Authors:
GUAN Ya-feng
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| Cite this article: |
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YANG Bing-cheng,TAN Feng,GUAN Ya-feng. A Novel Light-Emitting Diode-Induced Fluorescence Detector for Micro-Scale Flow Analysis System[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(12): 1995-1997.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2005/V25/I12/1995 |
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