| 光谱学与光谱分析 |
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| Spectrophotometric Study on the Interaction of Acridine Orange and Heparin |
| SUN Wei, DING Ya-qin, TENG Wen-hui, JIAO Kui |
| College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China |
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Abstract The interaction of acridine orange (AO) and heparin was studied by spectrophotometry. In the pH 2.0 Britton-Robinson (B-R) buffer solution, AO had a maximum absorption at 478 nm and 492 nm. After the addition of heparin, the absorbance value at 492 nm decreased greatly and a new absorption peak at 453 nm appeared, indicating a strong interaction taking place in the reaction solution. Under the optimal condition, the decrease in absorbance at 492 nm was linear with the concentration of heparin in the range of 3.0-15.0 mg·L-1. The molar absorptivity was calculated to be ε=1.599×105 L·mol-1·cm-1 and the detection limit was 0.236 mg·L-1 (3σ). The method was furthur applied to the detection of heparin sodium injection samples with satisfactory results. The binding number was calculated to be 1∶3 with molar ratio method.
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Received: 2005-10-08
Accepted: 2006-01-16
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Corresponding Authors:
SUN Wei
E-mail: sunwei@qust.edu.cn
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| Cite this article: |
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SUN Wei,DING Ya-qin,TENG Wen-hui, et al. Spectrophotometric Study on the Interaction of Acridine Orange and Heparin[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(01): 116-119.
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https://www.gpxygpfx.com/EN/Y2007/V27/I01/116 |
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