| 光谱学与光谱分析 |
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| Spatial Orientation Interaction Mechanism of Three Component Complex of Protein ——BSA/SDS/Azur a System |
| CAO Wen-gen1, 2, JIAO Qing-cai1,LIU Qian1 |
1. Pharmaceutic Biotechnology Key Laboratory, College of Life Science, Nanjing University, Nanjing 210093, China
2. Department of Biochemistry, Suzhou College, Suzhou 234000, China |
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Abstract The interaction of bovine serum albumin (BSA)，sodium dodecyl sulphate (SDS) and Azur A(AA) was studied by spectral probe. The influence of the molar ratio of SDS/BSA on the absorption spectra of BSA-SDS-AA complex was investigated. It was found that the new absorption peak and color changes generated by BSA-SDS-AA complex arose from the difference in the spatial orientation aggregative degree of AA binding on BSA-SDS-AA complex.
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Received: 2003-06-15
Accepted: 2004-11-21
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Corresponding Authors:
CAO Wen-gen
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| Cite this article: |
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CAO Wen-gen,JIAO Qing-cai,LIU Qian. Spatial Orientation Interaction Mechanism of Three Component Complex of Protein ——BSA/SDS/Azur a System[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(09): 1478-1481.
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https://www.gpxygpfx.com/EN/Y2005/V25/I09/1478 |
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