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| Analysis of Mid-Infrared Spectral Characteristics of Soils Cultivated With Salvia Miltiorrhiza at Different Intervals Based on Infrared Spectroscopy |
| QIAO Lu1, 2, LIU Yong-hong1, 2, XU Ke-ke1, 2, YU Huan-ying1, CHEN Yuan-jie3, YANG Lin-lin1, 2, DONG Cheng-ming1, 2*, WANG Lei1* |
1. School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China
2. Henan Provincial Ecological Planting Engineering Technology Research Center of Authentic Medicinal Materials, Zhengzhou 450046, China
3. School of Pharmacy, Henan University, Kaifeng 475000, China
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Abstract The continuous cropping obstacle caused by the differential changes in beneficial and pathogenic bacteria in the rhizosphere mediated by root exudates is the main factor restricting the development of Salvia miltiorrhiza cultivation. Propose a method based on infrared spectroscopy technology combined with two-dimensional correlation spectroscopy to quickly detect the composition and content changes of root exudates of Salvia miltiorrhiza. The spectra of 7 kinds of Salvia miltiorrhiza soil samples in the same area (not planted with Salvia miltiorrhizae), 1 year of rotation (19-ED, 23-ED), 2 years of rotation (23-One), 3 years of rotation (23-Two), 5 years of rotation (19-Five), and Salvia miltiorrhiza (19-ING) were collected, and the characteristics of the extraction were analyzed. The composition of soil compounds was analyzed. The results showed that the infrared spectral peaks and peak shapes of salvia miltiorrhiza cultivated at different intervals were the same. The main characteristic absorption peaks were around 3 622, 3 380, 1 638, 995, 777, 693, 524 and 463 cm-1, respectively, to characterize the functional groups of phenolic hydroxy —OH, carbonyl C═O, methylene, benzene ring absorption substitution, cyclic ketones and other substances in phenolic acids. The absorbance of the soil with 1 year of rotation was the strongest at each characteristic peak, indicating that the autotoxic substances such as phenolic acids and esters continued to accumulate during the planting process. The position, number, and color of the absorption peaks were different in the bands 3 750~3 600, 2 170~2 145, 2 060~2 030, and 530~585 cm-1, which clearly characterized the differences in functional groups. This indicates that the combination of infrared and 2D correlation spectroscopy can rapidly detect and monitor organic compounds in soil, and provide a theoretical basis for exploring the formation and reduction mechanisms of continuous cropping obstacles in Salvia miltiorrhiza.
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Received: 2023-11-22
Accepted: 2024-05-29
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Corresponding Authors:
DONG Cheng-ming, WANG Lei
E-mail: dcm88@sina.com；838133668@qq.com
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