| 光谱学与光谱分析 |
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| Spectral Analysis and Synthesis of Artificial Antigen of the Organophosphors Pesticide Methamidophos |
| WANG Xiang-hong1,2,DUAN Zhen-juan1,SONG Shi-ying1,WANG Shuo1* |
1. Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Food Science and Biologic Technology College, Tianjin 300457, China
2. Agricultural University of Hebei, Food Science and Technology College, Baoding 071001, China |
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Abstract In the present paper, a hapten of methamidophos was synthesized and conjugated with KLH by active ester method, thus the first artificial antigen was obtained. By diazotization method methamidophos conjugated with BSA, and the second artificial antigen was obtained. The synthesized haptens were characterized by MS, IR and 1H NMR, and the two artificial antigens were determined by the method of IR spectrum. The result implied that both the artificial antigens have absorbance peaks of hapten and protein, indicating that they were prepared successfully. This could provide evidence that the method of IR spectrum can be used to determine whether the artificial antigens are synthesized successfully.
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Received: 2005-12-10
Accepted: 2006-04-20
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Corresponding Authors:
WANG Shuo
E-mail: s.wang@tust.edu.cn
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| Cite this article: |
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WANG Xiang-hong,DUAN Zhen-juan,SONG Shi-ying, et al. Spectral Analysis and Synthesis of Artificial Antigen of the Organophosphors Pesticide Methamidophos[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(05): 881-885.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2007/V27/I05/881 |
[1] TANG Chu-chi, LI Yu-chang, CHEN Bin, et al(唐除痴，李煜昶, 陈 彬 ，等). Pesticide Chemistry(农药化学). Tianjin: Nankai University Publishing House(天津: 南开大学出版社)，1998. 3.
[2] DONG Guo-wei, LIU Xian-jin, et al(董国伟，刘贤进, 等). Journal of Huazhong Agricultural University(华中农业大学学报), 2001,20(4): 340.
[3] DONG Guo-wei, LIU Xian-jin, et al(董国伟，刘贤进, 等). Journal of Huazhong Agricultural University(华中农业大学学报)，2001，20(5)：434.
[4] SUN Yuan-ming, ZHANG Chun-yan, et al(孙远明，张春艳, 等). Immunological Journal(免疫学杂志), 2002, 18(3): 163.
[5] ZHAO Su-qing, SUN Yuan-ming, et al(赵肃清，孙远明, 等). Immunological Journal(免疫学杂志), 2003, 19(2): 142.
[6] ZHAO Su-qing, CAI Yan-fei, et al(赵肃清，蔡燕飞, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004, 24(2): 207.
[7] Lee J K, Ahn K C, Stoutamire D W. et al. Journal of Agricultural and Food Chemistry, 2003, 51(13): 3695.
[8] LIU Feng-quan, XU Zhi-gang, et al(刘凤权，许志刚，等). Journal of Agricultural Biotechnology(农业生物技术学报)，1998，6(2)：141.
[9] LI Jun-suo, LI Xi-wang, et al(李俊锁，李喜旺，等). Journal of China Agricultural University(中国农业大学学报)，1999，4(1)：109.
[10] ZHOU Xiang-yang, LIN Chun-zhong, et al(周向阳，林纯忠，等). Food Science(食品科学)，2004，25(1)：151.
[11] ZHOU Pei, LU Yi-tong(周 培，陆贻通). Chinese Journal of Pesticides(农药)，2002，41(5)：12.
[12] Wang S, Allan R D, Skerritt J H, et al. Journal of Agricultural and Food Chemistry, 1998, 46: 3330.
[13] Wang S, Allan R D, Skerritt J H, et al. Journal of Agricultural and Food Chemistry, 1999, 47: 3416.
[14] ZHU Guo-nian, WU Yin-liang, et al(朱国念，吴银良，等). Journal of Zhejiang University(Agric. & Life Sci.)(浙江大学学报·农业与生命科学版)，2002，28(1)：47.
[15] ZHOU Si-xiang, LIU Fu-cheng(周思祥，刘福成). Chinese Journal of Pesticides(农药)，2005，44(8)：337.
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