| 光谱学与光谱分析 |
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| Determination of 8-Hydroxy-2’-Deoxyguanosine in Urine with Protamine Using Resonance Energy Transfer Fluorescence Quenching by Sensitizing Effect of Triton X-100 |
| MI Xian-wen1,3,WANG Yong-sheng1,2*,XUE Jin-hua1,OUYANG Yun-fu1,YANG Sheng-yuan1,WANG Ying1 |
1. College of Public Health,Nanhua University,Hengyang 421001,China
2. College of Chemistry and Chemical Engineering,Nanhua University，Hengyang 421001,China
3. Huaihua Medical College,Huaihua 418000,China |
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Abstract The optimum conditions for the resonance energy transfer between 8-hydroxy-2’-deoxyguanosine(8-OHdG) and protamine were studied,and a novel method for the determination of 8-OHdG has been developed based on the resonance energy transfer fluorescence quenching effect of protamine. The fluorescence quenching intensity of Ps at 301 nm was proportional to the concentration of 8-OHdG. The linear range of the calibration curve was 0.50-7.1 μg·mL-1 in the pH 8.2 Tris-HCl buffer solution with the detection limits of 0.18 μg·mL-1. The relative standard deviation was 6.2% and the average recovery was 104%(n=6). The proposed method is simple and sensitive,and has succeeded in analyzing the spiked urine sample with satisfactory results.
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Received: 2006-06-27
Accepted: 2006-10-09
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Corresponding Authors:
WANG Yong-sheng
E-mail: yongsheng.w@tom.com
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| Cite this article: |
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MI Xian-wen,WANG Yong-sheng,XUE Jin-hua, et al. Determination of 8-Hydroxy-2’-Deoxyguanosine in Urine with Protamine Using Resonance Energy Transfer Fluorescence Quenching by Sensitizing Effect of Triton X-100 [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2007, 27(08): 1570-1573.
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| URL: |
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https://www.gpxygpfx.com/EN/Y2007/V27/I08/1570 |
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