| 光谱学与光谱分析 |
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| Spectral Study on the Interaction Mechanism between Thionine and Calf Thymus DNA |
| DU Jiang-yan1, HUANG Xiao-hua2, XU Fei2, FENG Yu-ying2, XING Wei1, LU Tian-hong1, 2* |
1. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
2. Department of Chemistry and Environmental Science, Nanjing Normal University, Nanjing 210097, China |
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Abstract The interaction mechanism of thionine (TH) and calf thymus DNA (CT-DNA) was studied with UV-Vis absorption spectroscopy, fluorescence spectroscopy, circular dichroism (CD), and X-ray photoelectron spectroscopy (XPS). It was demonstrated that in the pH 7.2 phosphate buffer solution, the interaction of TH and CT-DNA is in the intercalative mode. The absorbance of the absorption peak of TH decreases and the peak position shifts to the red direction. The binding constant (K) of TH with CT-DNA is 1.45×104 mol·L-1. The fluorescence intensities of TH decrease drastically with increasing the concentration of CT-DNA. The Stern-Volmer quenching constant (KSV) was calculated to be 1.01×104 mol·L-1. The intercalative sites are mainly located at the G-C sequences of the CT-DNA molecule through the S atom in the TH molecule according to the experimental data obtained from the fluorescence spectra and XPS analysis. The secondary structure conformation of CT-DNA is changed due to its interaction with TH.
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Received: 2004-04-09
Accepted: 2004-07-13
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Corresponding Authors:
LU Tian-hong
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| Cite this article: |
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DU Jiang-yan,HUANG Xiao-hua,XU Fei, et al. Spectral Study on the Interaction Mechanism between Thionine and Calf Thymus DNA [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(09): 1435-1438.
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https://www.gpxygpfx.com/EN/Y2005/V25/I09/1435 |
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