|
|
|
|
|
|
Study on Fluorescence Quenching and Absorption Spectra of Olive Oil |
WANG Hong-peng, WAN Xiong* |
Key Laboratory of Space Active Opto-Electronics Technology, Shanghai Institute of Technical Physics of the Chinese Academy of Sciences, Shanghai 200083, China |
|
|
Abstract The olive oil, known as “liquid gold”, has become a synonym for healthy edible oil. It not only has a steep increase in its price, but also has become a best-selling oil in the non producing market. Spectral method has many advantages compared with other technologies, such as fast, nondestructive and non sample processing. Different spectral detection methods have a particular emphasis on the material components, for example, infrared spectroscopy focuses on the detection of fatty acid content, Raman spectroscopy focuses on the detection of molecules, fluorescence spectroscopy focuses on the detection of photosensitive substances, and absorption spectroscopy focuses on the detection of unsaturated fatty acids. Fluorescence and absorption spectra are very sensitive to photosensitive substances, and olive oil is rich in chlorophyll and other photosensitive substances. Therefore, fluorescence and absorption spectra have become an effective technique for identifying olive oil. Chlorophyll is an organic molecule containing the structure of cycloporphyrin. The molecular structure of this kind of molecular structure has the characteristics of absorption of light, and the absorption spectra of different kinds of chlorophyll are unique, among which the content of chlorophyll a in green plants is the most. In order to study the application of the absorption spectrum of chlorophyll and the fluorescence characteristics of the extra virgin olive oil, the content of chlorophyll in olive oil was indirectly regulated by mixing different proportion of corn oil in the special primary olive oil. The fluorescence and absorption spectra of different adulterated olive oil were measured and the phase of chlorophyll concentration was studied. The effecus of chlorophyll concentration and adulteration amount on the absorption spectra and fluorescence characteristics of olive oil were studied. 10 samples of the same batch of extra virgin olive oil were taken, 9 of them were diluted in equal proportion and 10 samples were sequentially ordered according to adulteration. The fluorescence and absorption spectra of the 10 samples were collected in turn, and the correlation between the concentration of chlorophyll and adulteration were compared and the effects of the two spectral techniques on the identification of olive oil were compared. With the increase of chlorophyll concentration, the fluorescence intensity becomes stronger and weakens sharply after a certain time, that is, the aggregation fluorescence quenching. This phenomenon is mainly due to the intermolecular π—π action caused by the molecular structure of the phyphyrphyrin, which makes the non excited low energy molecules and high energy molecules stacked together. The radiation transition of energy (Fluorescence) is also transformed into the energy transfer (heat exchange) between the molecules. As for the absorption spectrum, the intensity of absorption spectrum increases with the increase of chlorophyll concentration. The main energy of the absorption of chlorophyll in olive oil consists of two parts, including the emission of magnesium electron emission and the intermolecular heat exchange, while the absorption spectrum of olive oil does not appear like aggregation fluorescence quenching, and there is an approximate linear correlation between the absorption spectrum intensity and the adulteration concentration. The results show that when the fluorescence quenching occurs, the energy of the absorption of chlorophyll is still linearly related to the concentration, and the efficiency of heat exchange caused by the stacking of high and low energy molecules increases.
|
Received: 2018-06-04
Accepted: 2018-10-11
|
|
Corresponding Authors:
WAN Xiong
E-mail: wanxiong@mail.sitp.ac.cn
|
|
[1] Internet: Codex Standard for Table Olives. (Codex Stan. 66-1981). Downloaded from http://www.codexalimentarius.org/input/download/standards/243/CXS_066e.pdf.on 25/9/2013.
[2] GB 23347—2009. National Standards of the People’s Republic of China(中华人民共和国国家标准). Olive Oil and Olive-Pomace Oil(橄榄油、油橄榄果渣油).
[3] Carranco N, Farrés-Cebrián M, Saurina J, et al. Foods, 2018, 7(4): 44.
[4] Jiang Z, Wang Y, Zheng Y, et al. Journal of Separation Science, 2016, 39(15): 2928.
[5] Sales C, Cervera M I, Gil R, et al. Food Chemistry, 2017, 216: 365.
[6] Mora-Ruiz M E, Reboredo-Rodríguez P, Salvador M D, et al. European Journal of Lipid Science and Technology, 2017, 119(1):1600099.
[7] Georgouli K, Del Rincon J M, Koidis A. Food Chemistry, 2017, 217: 735.
[8] Ferreiro-González M, Barbero G F, álvarez J A, et al. Food Chemistry, 2017, 220: 331.
[9] Aparicio-Ruiz R, Tena N, Romero I, et al. Grasasy Aceites, 2017, 68(4): 219.
[10] Merás I D, Manzano J D, Rodríguez D A, et al. Talanta, 2018, 178: 751.
[11] Zhu W, Wang X, Chen L. Food Chemistry, 2017, 216: 268.
[12] Rodrigues N, Dias L G, Veloso A C A, et al. European Food Research & Technology, 2017, 243(4): 597. |
[1] |
ZHENG Hong-quan, DAI Jing-min*. Research Development of the Application of Photoacoustic Spectroscopy in Measurement of Trace Gas Concentration[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 1-14. |
[2] |
BAI Xi-lin1, 2, PENG Yue1, 2, ZHANG Xue-dong1, 2, GE Jing1, 2*. Ultrafast Dynamics of CdSe/ZnS Quantum Dots and Quantum
Dot-Acceptor Molecular Complexes[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 56-61. |
[3] |
ZHENG Pei-chao, YIN Yi-tong, WANG Jin-mei*, ZHOU Chun-yan, ZHANG Li, ZENG Jin-rui, LÜ Qiang. Study on the Method of Detecting Phosphate Ions in Water Based on
Ultraviolet Absorption Spectrum Combined With SPA-ELM Algorithm[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 82-87. |
[4] |
LEI Hong-jun1, YANG Guang1, PAN Hong-wei1*, WANG Yi-fei1, YI Jun2, WANG Ke-ke2, WANG Guo-hao2, TONG Wen-bin1, SHI Li-li1. Influence of Hydrochemical Ions on Three-Dimensional Fluorescence
Spectrum of Dissolved Organic Matter in the Water Environment
and the Proposed Classification Pretreatment Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 134-140. |
[5] |
LIU Jia, ZHENG Ya-long, WANG Cheng-bo, YIN Zuo-wei*, PAN Shao-kui. Spectra Characterization of Diaspore-Sapphire From Hotan, Xinjiang[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 176-180. |
[6] |
XIA Ming-ming1, 2, LIU Jia3, WU Meng1, 2, FAN Jian-bo1, 2, LIU Xiao-li1, 2, CHEN Ling1, 2, MA Xin-ling1, 2, LI Zhong-pei1, 2, LIU Ming1, 2*. Three Dimensional Fluorescence Characteristics of Soluble Organic Matter From Different Straw Decomposition Products Treated With Calcium Containing Additives[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 118-124. |
[7] |
YANG Guang1, JIN Chun-bai1, REN Chun-ying2*, LIU Wen-jing1, CHEN Qiang1. Research on Band Selection of Visual Attention Mechanism for Object
Detection[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 266-274. |
[8] |
GU Yi-lu1, 2,PEI Jing-cheng1, 2*,ZHANG Yu-hui1, 2,YIN Xi-yan1, 2,YU Min-da1, 2, LAI Xiao-jing1, 2. Gemological and Spectral Characterization of Yellowish Green Apatite From Mexico[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 181-187. |
[9] |
HAN Xue1, 2, LIU Hai1, 2, LIU Jia-wei3, WU Ming-kai1, 2*. Rapid Identification of Inorganic Elements in Understory Soils in
Different Regions of Guizhou Province by X-Ray
Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 225-229. |
[10] |
GAO Hong-sheng1, GUO Zhi-qiang1*, ZENG Yun-liu2, DING Gang2, WANG Xiao-yao2, LI Li3. Early Classification and Detection of Kiwifruit Soft Rot Based on
Hyperspectral Image Band Fusion[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 241-249. |
[11] |
WU Hu-lin1, DENG Xian-ming1*, ZHANG Tian-cai1, LI Zhong-sheng1, CEN Yi2, WANG Jia-hui1, XIONG Jie1, CHEN Zhi-hua1, LIN Mu-chun1. A Revised Target Detection Algorithm Based on Feature Separation Model of Target and Background for Hyperspectral Imagery[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 283-291. |
[12] |
LIU Wei1, 2, ZHANG Peng-yu1, 2, WU Na1, 2. The Spectroscopic Analysis of Corrosion Products on Gold-Painted Copper-Based Bodhisattva (Guanyin) in Half Lotus Position From National Museum of China[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3832-3839. |
[13] |
LU Wen-jing, FANG Ya-ping, LIN Tai-feng, WANG Hui-qin, ZHENG Da-wei, ZHANG Ping*. Rapid Identification of the Raman Phenotypes of Breast Cancer Cell
Derived Exosomes and the Relationship With Maternal Cells[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3840-3846. |
[14] |
SHEN Si-cong, ZHANG Jing-xue, CHEN Ming-hui, LI Zhi-wei, SUN Sheng-nan, YAN Xue-bing*. Estimation of Above-Ground Biomass and Chlorophyll Content of
Different Alfalfa Varieties Based on UAV Multi-Spectrum[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3847-3852. |
[15] |
WANG Hong-jian1, YU Hai-ye1, GAO Shan-yun1, LI Jin-quan1, LIU Guo-hong1, YU Yue1, LI Xiao-kai1, ZHANG Lei1, ZHANG Xin1, LU Ri-feng2, SUI Yuan-yuan1*. A Model for Predicting Early Spot Disease of Maize Based on Fluorescence Spectral Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3710-3718. |
|
|
|
|