|
|
|
|
|
|
A Highly Sensitive Label-Free Quantitative Detection Method for Tumor Marker Based on Au NRs/PMMA Substrate |
TONG Li-ying1, FU Hao1, PENG Le1, LI Qing-ning2, SHI Qing-hua3, ZHOU Jun1* |
1. Department of Microelectronics Science & Engineering, Faculty of Science, Ningbo University, Ningbo 315211, China
2. Department of Biochemistry and Molecular Biology, Medical School, Ningbo University, Ningbo 315211, China
3. Affiliated Hospital, School of Medicine, Ningbo University, Ningbo 315020, China |
|
|
Abstract Preparation of high-quality noble nanostructure substrate is vital for the application of surface-enhanced Raman scattering (SERS) technology in ultrasensitive bioassay. In our work, based on the improved Langmuir-Blodgett method, the gold nanorods were extracted from colloid to the interface between the colloid and toluene with the help of ethanol, and fixed by polymethyl methacrylate (PMMA), then a uniform and dense array of two-dimensional domain-like nanostructure was formed in a large area. Next, the plasma clean technology was used to treat the fabricated substrate for enhancing its SERS performance due to the exposed surface of the Au NRs. The experimental results showed that the Au NRs/PMMA substrate exhibited the excellent SERS characteristic and its enhancement factor (EF) achieved 5.49×106 under irradiating of 785 nm laser. In addition, the highly sensitive label-free quantitative detection of tumor maker, prostate specific antigen (PSA), was developed by using Au NRs/PMMA substrate. In the experiments of label-free detection, the Raman characteristic peaks of the PSA were first acquired by comparing the SERS spectra of the PSA standard solution and new-born battle serum solution, and they were mainly located at 823, 1 080, 1 385, 1 586 and 1 640 cm-1. Following, the SERS spectra of PSA standard solution, clinical male serum samples and female serum samples were measured and analyzed to screen the Raman characteristic peaks of PSA associated only with serum PSA levels, and they were located at 649,680 and 1 640 cm-1. Furthermore, the SERS spectra of α-fetoprotein (AFP) belonging to the glycoprotein same with PSA and human kallikrein 2 (hK2) homologous with PSA were separately measured as two controls, and the extremely specific Raman characteristic peaks of PSA located at 1640 cm-1 were determined and applied in the detection of clinic serum samples. Subsequently, the does-repose curve was obtained by the relationship of the intensities of the Raman peaks at 1640 cm-1 and the PSA concentrations in the standard solutions. Lastly, the PSA concentrations in the clinical serum samples were detected based on the SERS-based label-free detect proposal. It demonstrated that SERS-based label-free detection not only exhibits a well consistency of test data when compared with that of the chemiluminescent immunoassay (CLIA), but also higher sensitivity, and its limit of detection as low as 0.06 ng·mL-1 in the range of 0.1 mg·mL-1~0.1 ng·mL-1. Therefore, it reveals that the proposed protocol has a significant application potential for the quantitative detection of tumor marker.
|
Received: 2018-03-19
Accepted: 2018-07-14
|
|
Corresponding Authors:
ZHOU Jun
E-mail: zhoujun@nbu.edu.cn
|
|
[1] HE Jie, CHEN Wan-qing(赫 捷,陈万青). Annual Report on Status of Cancer in China(中国肿瘤登记年报). Beijing: Tsinghua University Press(北京:清华大学出版社),2017.
[2] Chen W Q, Zheng R S, Baada P D, et al. CA-Cancer J. Clin, 2016, 66: 115.
[3] Setiawan D, Luttjeboer J, Pouwels K B, et al. JPN. J. Clin. Oncol., 2017, 47(3): 265.
[4] Skenandore C S, Pineda A, Bahr J M, et al. Domest. Anim. Endoc., 2017, 60: 61.
[5] Li X G, Meng M, Zheng L, et al. Anal. Chem., 2016, 88(17): 8556.
[6] Ding S Y, Yi J, Li J F, et al. Nature Reviews Materials, 2016, 1: 1.
[7] Li J F, Zhang Y J, Ding S Y, et al. Chem. Rev., 2017, 117(7): 5002.
[8] Wang Z Y, Zong S F, Wu L, et al. Chem. Rev., 2017, 117(12): 7910.
[9] Wang Y, Kang S, Khan A, et al. Sci. Rep., 2016, 6: 21242.
[10] Wang Y W, Doerksen J D, Kang S, et al. Small, 2016, 12(40): 5612.
[11] Liu Y, Zhou H B, Hu Z W, et al. Biosens. Bioelestro., 2017, 94: 131.
[12] Lane L A, Qian X M, Nie S M. Chem. Rev., 2015, 115(19): 10489.
[13] Li S X, Zhang Y J, Xu J F, et al. Appl. Phys. Lett., 2014, 105(9): 091104.
[14] Chaloupková Z, Balzerová A, Baǐinková J, et al. Anal. Chim. Acta, 2018, 997: 44.
[15] Falk Y Z, Schmitt J, Alfredsson V. Micropor. Mesopor. Mat., 2018, 256: 32.
[16] Allen J M, Xu J P, Blahove M, et al. J. ColloidInterf. Sci., 2017, 505: 1172.
[17] Erik C Dreaden, Alaaldin M Alkilany, Huang Xiaohua, et al. Chem. Soc. Rev., 2012, 41(7): 2740.
[18] Álvarez-Puebla R A. J. Phys. Chem. Lett., 2012, 3(7): 857.
[19] Shrivastava A, Gupta V B. Chronicles of Young Scientists, 2011, 2(1): 21. |
[1] |
LAI Chun-hong*, ZHANG Zhi-jun, WEN Jing, ZENG Cheng, ZHANG Qi. Research Progress in Long-Range Detection of Surface-Enhanced Raman Scattering Signals[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2325-2332. |
[2] |
LI Jia-jia, XU Da-peng *, WANG Zi-xiong, ZHANG Tong. Research Progress on Enhancement Mechanism of Surface-Enhanced Raman Scattering of Nanomaterials[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(05): 1340-1350. |
[3] |
SUN Zhi-ming1, LI Hui1, FENG Yi-bo1, GAO Yu-hang1, PEI Jia-huan1, CHANG Li1, LUO Yun-jing1, ZOU Ming-qiang2*, WANG Cong1*. Surface Charge Regulation of Single Sites Improves the Sensitivity of
Raman Detection[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(04): 1075-1082. |
[4] |
YIN Xiong-yi1, SHI Yuan-bo1*, WANG Sheng-jun2, JIAO Xian-he2, KONG Xian-ming2. Quantitative Analysis of Polycyclic Aromatic Hydrocarbons by Raman Spectroscopy Based on ML-PCA-BP Model[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(03): 861-866. |
[5] |
SUN Nan, TAN Hong-lin*, ZHANG Zheng-dong, REN Xiang, ZHOU Yan, LIU Jian-qi, CAI Xiao-ming, CAI Jin-ming. Raman Spectroscopy Analysis and Formation Mechanism of Carbon
Nanotubes Doped Polyacrylonitrile/Copper Cyclized to Graphite
at Room Temperature[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(09): 2983-2988. |
[6] |
WANG Zi-xiong, XU Da-peng*, ZHANG Yi-fan, LI Jia-jia. Research Progress of Surface-Enhanced Raman Scattering Detection Analyte Molecules[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(02): 341-349. |
[7] |
WAN Xiao-ming1, 2, ZENG Wei-bin1, 2, LEI Mei1, 2, CHEN Tong-bin1, 2. Micro-Distribution of Elements and Speciation of Arsenic in the Sporangium of Pteris Vittata[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(02): 470-477. |
[8] |
HUANG Hui1, 2, TIAN Yi2, ZHANG Meng-die1, 2, XU Tao-ran2, MU Da1*, CHEN Pei-pei2, 3*, CHU Wei-guo2, 3*. Design and Batchable Fabrication of High Performance 3D Nanostructure SERS Chips and Their Applications to Trace Mercury Ions Detection[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(12): 3782-3790. |
[9] |
FU Xing-hu, WANG Zhen-xing, MA Shuang-yu, ZHAO Fei, LU Xin, FU Guang-wei, JIN Wa, BI Wei-hong. Preparation and Properties of Micro-Cavity Silver Modified Fiber SERS Probe[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(09): 2800-2806. |
[10] |
GUI Bo1, 2, YANG Yu-dong1, ZHAO Qian1, 2, SHI Meng1, MAO Hai-yang1, 3*, WANG Wei-bing1, CHEN Da-peng1, 3. A SERS Substrate for On-Site Detection of Trace Pesticide Molecules Based on Parahydrophobic Nanostructures[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(08): 2499-2504. |
[11] |
SUN Ning, CHEN Jun-fan, ZHANG Jie*, ZHU Yong. The Forming Mechanism of Surface Morphology of Nanostructures and Its Effect on Graphene Raman Spectra[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(06): 1821-1827. |
[12] |
ZHANG Can, ZHANG Jie*, DOU Xin-yi, ZHU Yong. Connection of Absorption and Raman Enhancement Characteristics of Different Types of Ag Nanoparticles[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(06): 1816-1820. |
[13] |
DOU Xin-yi, ZHANG Can, ZHANG Jie*. Effects of Process Parameters on Double Absorption Resonance Peaks of Au Nanoparticles[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(05): 1446-1451. |
[14] |
ZHANG Lei, ZHANG Xia*, WENG Yi-jin, LIU Xiao. Preparation and Properties of Ag/PANI Multifunction Nanozymes[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(11): 3399-3403. |
[15] |
TIAN Hui-yan1,LIU Yu1, HUANG Jiao-qi1, XIE Feng-xin1, HUANG Guo-rong1, LIAO Pu1, FU Wei-ling1, ZHANG Yang2*. Research Progress and Application of Surface-Enhanced Raman Scattering Technique in Nucleic Acid Detection[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(10): 3021-3028. |
|
|
|
|