| 光谱学与光谱分析 |
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| Investigation of Fucoidanase by FTIR Spectra |
| MA Shuang1,2,XIAO Hou-rong2,WU Qian-qian2,PAN Ren-rui2,CAI Jing-min1,2* |
1. College of Life Science,Anhui Agricultural University,Hefei 230036，China
2. Department of Biology and Environment Engineering，Hefei College，Hefei 230022，China |
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Abstract Fucoidanase was isolated and purified from marine fungus LD8 by solid state fermentation,extraction with citric acid buffer,acetone precipitatation and column chromatography on Sephadex G-100. A single band on PAGE shows that pretty pure fucoidanase has been obtained. FT-IR spectra and its derivation，self-deconvolution and curve-fitting methods were used to analyze the secondary structure of the fucoidanase. Composite bands of the amide Ⅰ and amide Ⅲ were studied by using Fourier self-deconvolution (FSD) with an enhancement factor of K=2.2 and a half width of 20.2 cm-1. The relative average fractions of α-helix，β-sheet，random coil，β-turn are 11.5%,58.6%,14.5% and 15.9%,respectively,according to amide Ⅰ region,while the content of α-helix is 12%，β-sheet 57.3%，random coil 14.5%，and β-turn 16.3% on amide Ⅲ region. In other words,both the conclusions were exactly consistent. All the above results show that β-sheet was the dominant component，which is about 58%，and that β-turn is about 15%,random coil 15%，and α-helix 12% at room temperature.
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Received: 2006-11-10
Accepted: 2007-02-20
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Corresponding Authors:
CAI Jing-min
E-mail: caijingmin@hfuu.edu.cn
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