光谱学与光谱分析 |
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The Research on the Quenching of Fluorescence Spectra of TOPⅠ by Iodine Anion |
XIA Bing-le1, PENG Dun-geng1, LI Min-li1, XIAO Hong2, WANG Kai-bao3 |
1. Department of Chemistry, USTC,Hefei 230026, China 2. Zhumadian Cigarette Factory,Zhumadian 463000, China 3. Wuhu Cigarette Factory,Wuhu 241000, China |
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Abstract Comparing the fluorescence spectra of tobacco peroxidaseⅠ(TOPⅠ) solution and the solutions titrated by iodine anions,the number of binding locus and the binding constant of iodine anion to TOPⅠ were calculated by using the modified Stern-Volmer equation. The mechanism of the quenching of fluorescence spectra and the distribution of tryptophane residues in the TOPⅠ molecule were discussed.
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Received: 2003-09-23
Accepted: 2004-02-06
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Corresponding Authors:
XIA Bing-le
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Cite this article: |
XIA Bing-le,PENG Dun-geng,LI Min-li, et al. The Research on the Quenching of Fluorescence Spectra of TOPⅠ by Iodine Anion[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(02): 239-241.
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URL: |
https://www.gpxygpfx.com/EN/Y2005/V25/I02/239 |
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