|
|
|
|
|
|
| The Cysteine-Protected Copper Nanoclusters for Rapid Detection of p-Nitrophenol |
| HE Xuan, OU Li-juan*, XIE Yu-dan, LUO Jian-xin, DU Cong, ZHANG Chun-yan |
School of Material Science and Engineering, Hunan Institute of Technology, Hengyang 421002, China
|
|
|
|
|
Abstract 4-nitrophenol (4-NP), a phenolic compound with highly toxic and carcinogenic properties, has become a global concern. As a fluorescence probe, a rapid and sensitive fluorescence assay for 4-NP was developed using cysteine-protected copper nanoclusters (Cys-CuNCs). The Cys-CuNCs were prepared using ascorbic acid-capped copper nanoparticles (CuNPs) as a precursor and cysteine as an etching agent. The prepared Cys-CuNCs showed excellent fluorescence properties and stability solubility with the maximum excitation and emission peak at 370 nm and 464 nm, respectively. In the presence of 4-NP, the fluorescence of CuNCs was quenched effectively. Single-factor experiments were investigated to optimize the pH of the buffer and the reaction time for nitrophenol detection. The optimal conditions were as follows: phosphate buffer (10 mmol·L-1, pH 8.0) as a working buffer, mixing 4-NP and CuNCs for fluorescence scan. A sensitive analysis method for nitrophenol was constructed by utilizing the variation of fluorescence intensity F0-F/F0 as abscissa and 4-NP concentration as ordinate. The fluorescence quenching rate of CuNCs showed a linear relationship with the concentration of 4-NP in the range from 1 to 100 μmol·L-1 with the detection limit of 0.31 μmol·L-1. Four other 4-NP homologues and eight other organics were selected as the interferent; all of them had almost no effect on the fluorescence of CuNCs, indicating that the method had good anti-interference ability. In addition, the strategy was successfully applied to detect 4-NP in lake water with satisfactory recoveries from 97.0%～101%. The relative standard deviation was 2.71%～3.60%, indicating that the method is accurate and could be used for 4-NP detection in actual samples.
|
|
Received: 2023-11-17
Accepted: 2024-02-26
|
|
|
|
Corresponding Authors:
OU Li-juan
E-mail: 179355188@qq.com
|
|
[1] ZHANG Cui-zhong, ZHANG Zhen-fa, LIAN Huan, et al(张翠忠, 张贞发, 连 欢，等). J. Anal. Chem.(分析化学), 2017, 45(1): 28.
[2] HUANG Li(黄 俐) . Environment and Development(环境与发展), 2020, 32(7): 112.
[3] Mohammad F, Farshid N, Mahnaz A. Journal of Environmental Chemical Engineering, 2020, 8(4): 103948.
[4] Fischer J, Barek J, Wang J. Electroanalysis, 2006, 18(2): 195.
[5] Zhu S Y, Niu W X, Li H J, et al. Talanta, 2009, 79(5): 1441.
[6] Yang J M, Hu X W, Liu Y X, et al. Microporous and Mesoporous Materials, 2019, 274(1): 149.
[7] Zhang S T, Zhang D F, Ding Y F, et al. Analyst, 2019, 144(18): 5497.
[8] Ou L J, Qin S W, Bo H, et al. Anal. Methods, 2019, 11(18): 2410.
[9] Luo Q, Lin Y S, Cai Q H, et al. Analyst, 2022, 147(12): 2749.
[10] XIAO Shu-fen, WANG Zheng, WANG Xu-jun, et al(肖淑芬, 王 政, 王旭军). Chin. J. Anal. Chem.(分析化学), 2023, 51(3): 364.
[11] LI Yi-ming, ZHANG Guo-mei, ZHENG Yong-hao, et al(李一鸣, 张国梅, 郑永豪). J. Anal. Sci.(分析科学学报), 2022, 38(5): 545.
[12] TAO Bei-bei, WU Ning-ning, WANG Hai-bo(陶贝贝, 吴宁宁, 王海波). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2023, 43(10): 3158.
[13] Deng H H, Li K L, Zhuang Q Q, et al. Nanoscale, 2018, 10(14): 6467.
[14] Jia X F, Li J, Wang E K. Small, 2013, 9(22): 3873.
[15] Wang C, Yao Y G, Song Q J. Colloids and Surfaces B: Biointerfaces, 2016, 140(1): 373.
[16] WANG Hai-bo, TAO Bei-bei, WU Ning-ning, et al(王海波, 陶贝贝, 吴宁宁). Journal of Xinyang Normal University Natural Science Edition(信阳师范学院学报), 2022, 35(3): 464.
|
| [1] |
TAO Bei-bei, WU Ning-ning, WANG Hai-bo*. Highly Sensitive Determination of Rutin Based on Fluorescent Glutathione Stabilized Copper Nanoclusters[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3158-3162. |
| [2] |
ZHU Shao-hao1, SUN Xue-ping1, TAN Jing-ying1, YANG Dong-xu1, WANG Hai-xia2*, WANG Xiu-zhong1*. Study on a New Sensing Method of Colorimetric and Fluorescence Dual Modes for Pesticide Residue[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2785-2791. |
| [3] |
GUO He-qing1, 2, ZHANG Sheng-zi2*, LIU Xiao-meng2, JING Xu-feng1, WANG Hong-jun2. Research Progress of the Real-Time Detection System of Bioaerosols Based on Fluorescence Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2339-2347. |
| [4] |
LI Shi-lun1, LIU Tao2, SONG Wen-min3, WANG Tian-le2, LIU Wei1, CHEN Liang1, LI Zhi-gang2*, FENG Shang-shen1*. Study of Two-Dimensional Ordered Magnetic Co Nanosphere Array Film Construction and Its Optical Properties[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 2037-2042. |
| [5] |
OU Li-juan1*, LI Jing1, ZHANG Chao-qun1, LUO Jian-xin1, WEI Ji1, WANG Hai-bo2*, ZHANG Chun-yan1. Redox-Controlled Turn-on Fluorescence Sensor for H2O2 and Glucose Using DNA-Template Gold Nanoclusters[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(12): 3757-3761. |
| [6] |
LIU Pan1, 2, DU Mi-fang1, LI Zhi-ya1, GAO Ling-qing1, 3, HAN Hua-yun4, ZHANG Xin-yao1, 3. Determination of Trace Tellurium Content in Steel by Hydride Generation Atomic Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(10): 3103-3108. |
| [7] |
OU Li-juan, AN Xue-zhong, LUO Jian-xin, WANG Ling-yun, BO Heng, SUN Ai-ming, CHEN Lan-lan. High-Sensitive and Rapid Fluorescencet Detection of Hg2+ Based on Poly(adenine)-Templated Gold Nanoclusters[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(01): 164-167. |
| [8] |
SHI Ji-yong, LI Wen-ting, HU Xue-tao, SHI Yong-qiang, ZOU Xiao-bo*. A New Ratiometric Fluorescence Probe Based on CuNCs and CQDs and Its Application in the Detection of Hg2+[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(12): 3925-3931. |
| [9] |
GONG Ai-qin1*, JIN Dang-qin1, ZHU Xia-shi2. A Fluorescence Spectroscopic Study of the Interaction between Trelagliptin Succinate and Bovin Serum Albumine and Its Analytical Application[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(01): 157-160. |
| [10] |
MA Xue-hong1, SONG Bai-ling2, XIAO Wen-jun2, GENG Jing2, LI Xin-xia1*, CHEN Jian3. Study on Interaction between Alliin and Bovine and Hunman Serum Albumin with Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(06): 1826-1830. |
| [11] |
YU Wan-cheng, HU Xiao-bo*, CUI Ying-xin, CHEN Xiu-fang, XU Xian-gang . Laser Ablation of 6H-SiC Single Crystals and Spectral Characterization [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(04): 1255-1260. |
| [12] |
YU Wan-cheng, HU Xiao-bo*, CUI Ying-xin, CHEN Xiu-fang, XU Xian-gang . Laser Ablation of 6H-SiC Single Crystals and Spectral Characterization [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(04): 1255-1260. |
| [13] |
YU Wan-cheng, HU Xiao-bo*, CUI Ying-xin, CHEN Xiu-fang, XU Xian-gang . Laser Ablation of 6H-SiC Single Crystals and Spectral Characterization [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(04): 1255-1260. |
| [14] |
ZHANG Li-hua1, Arkin IBURAIM2* . The Establishment of the Method of the Fiber Optic Chemical Sensor Synchronous Absorption-Fluorescence[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(03): 755-758. |
| [15] |
XIONG Chao1,2, GE Liang-quan1*, LIU Duan3, ZHANG Qing-xian1, GU Yi1, LUO Yao-yao1, ZHAO Jian-kun1 . Influence of the Experiment Energy Dispersive X-Ray Fluorescence Measurement of Uranium by Different Excitation Source[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(03): 838-841. |
|
|
|
|
