Study on Aqueous Extracts of Three Kinds of Radix Puerariae in Clinical by 2D-IR Correlation Spectroscopy
XU Bei-lei1, SUN Su-qin2, ZHANG Gui-jun3, LI Wen-lan1, WANG Rui4, ZHANG Yan1, JIN Zhe-xiong1*, SONG Lin5
1. School of Pharmacy, Harbin University of Commerce, Harbin 150076, China
2. Department of Chemistry, Tsinghua University, Beijing 100084, China
3. College of Pharmaceutics, Beijing University of Chinese Medicine, Beijing 100102, China
4. Integrated Department, Harbin Stomatological Hospital, Harbin 150002, China
5. College of Mongolian Medicine, Inner Mongolia Medical University, Huhhot 010059, China
Abstract：Traditional Chinese medicine is a complicated chemical mixture system. It is helpful for quality control of traditional Chinese medicine to establish a fast and effective analysis method. There are three kinds of Radix Puerariae in clinical, which are Pueraria thomsonii Benth, Pueraria lobata (Willd. ) Ohwi and simmered Pueraria lobata (Willd.) Ohwi (processed Radix Puerariae). Furthermore, aqueous extract is an important method for Radix Puerariae in clinical application. Therefore, a macroscopic IR fingerprint method, conventional Fourier transform infrared spectroscopy (FTIR) combined with second derivative infrared spectroscopy (SD-IR) and two-dimensional correlation infrared spectroscopy (2D-IR), was applied to quickly analyze aqueous extracts of three kinds of Radix Puerariae in this study. The results showed that aqueous extract of Pueraria thomsonii Benth was different from aqueous extracts of Pueraria lobata (Willd.) Ohwi and simmered Pueraria lobata (Willd.) Ohwi by FTIR and SD-IR. There were differences of Pueraria lobata (Willd.) Ohwi after simmered by 2D-IR analysis. There were distinctive differences of main auto-peaks and cross-peaks in position and intensity. In the range of 1 800~1 300 cm-1, the strongest auto-peak was at 1 556 cm-1, and the second one appeared at 1 561 cm-1 in 2D-IR spectra of aqueous extracts of Pueraria lobata (Willd.) Ohwi. However, the strongest auto-peak was at 1 563 cm-1, and the second one appeared at 1 572 cm-1 in 2D-IR spectra of aqueous extracts of simmered Pueraria lobata (Willd.) Ohwi. In addition, there were two obvious auto-peaks at 1 536 and 1 634 cm-1, whose intensities were equal in 2D-IR spectra of aqueous extracts of Pueraria lobata (Willd.) Ohwi. The relative intensities of auto-peaks at 1 448 and 1 518 cm-1 were different in the two 2D-IR spectra. The cross-peaks at (1 518, 1 561) cm-1 and (1 518, 1 563) cm-1 of aqueous extract of Pueraria lobata (Willd.) Ohwi were stronger than those in 2D-IR of aqueous extract of simmered Pueraria lobata (Willd.) Ohwi. Therefore, aqueous extracts of Pueraria lobata (Willd.) Ohwi and simmered Pueraria lobata (Willd.) Ohwi can be identified intuitively by auto-peaks and cross peaks in 2D-IR spectra, and the change laws of functional groups of them can be revealed. It can provide the basis for the fast quality control of Radix Puerariae decoction in the process of clinical prescription application. The method including FTIR, SD-IR and 2D-IR, is rapid and exact, and can provide the means to analyze the complicated chemical mixture systems step by step.
徐蓓蕾，孙素琴，张贵君，李文兰，王 锐，张 琰，金哲雄，松 林. 处方应用三种葛根水煎液的二维相关红外光谱分析[J]. 光谱学与光谱分析, 2018, 38(03): 800-804.
XU Bei-lei, SUN Su-qin, ZHANG Gui-jun, LI Wen-lan, WANG Rui, ZHANG Yan, JIN Zhe-xiong, SONG Lin. Study on Aqueous Extracts of Three Kinds of Radix Puerariae in Clinical by 2D-IR Correlation Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(03): 800-804.
[1] The State of Pharmacopoeia Commission of the People’s Republic of China(中华人民共和国药典委员会编). Pharmacopoeia of the People’s Republic of China, Vol.1(中国药典, 一部), Beijing: Chemical Industry Press(北京: 化学工业出版社), 2015. 289.
[2] Xu Beilei, Zhang Guijun, Sun Suqin, et al. Journal of Molecular Structure, 2012, 1018: 88.
[3] Xu Beilei, Zhang Guijun, Xu Changhua, et al. Journal of Molecular Structure, 2015, 1096: 147.
[4] Sun Suqin, Chen Jianbo, Zhou Qun, et al. Planta Medica, 2010, 78(17): 1987.
[5] Liu Yan, Zhang Guijun, Sun Suqin, et al. Journal of Pharmaceutical and Biomedical Analysis, 2010, 52: 631.
[6] Qu Lei, Chen Jianbo, Zhang Guijun, et al. Spectrochim Acta part A Molecular and Biomolecular Spectroscopy, 2017, 174: 177.
[7] Xu Changhua, Liu Suli, Zhao Shengnan, et al. Planta Medica, 2013, 79: 1068.
[8] Chen Jianbo, Sun Suqin, Zhou Qun. Analytical and Bioanalytical Chemistry, 2014, 406(18): 4513.
[9] Xu Changhua, Wang Yang, Chen Jianbo, et al. Journal of Pharmaceutical and Biomedical Analysis, 2013, 74: 298.
[10] Liu Xinhu, Xu Changhua, Sun Suqin, et al. Spectrochim Acta Part A Molecular and Biomolecular Spectroscopy, 2012, 97: 290.
[11] Chen Jianbo, Sun Suqin, Zhou Qun. Food Chemistry, 2016, 212: 469.
