Investigation of Articular Cartilage Using FTIR Microspectroscopy for Guinea Pig with Spontaneous Osteoarthritis
YANG Li-ping1, ZHAO Tie-jun2, LIU Jian-ling3, WANG Jin-hong4, SONG Qing-hui1, ZHU Jia1, ZHANG Wan-qiang1, KONG Huan-yu1*
1. Pharmacology Laboratory of Traditional Chinese Medicine Orthopedics & Traumatology, Wangjing Hosptial, China Academy of Chinese Medical Sciences, Beijing 100700, China 2. Department of the 1st Orthopaedics Bone and Joint, Wangjing Hospital, China Academy of Chinese Medicine Sciences, Beijing 100102, China 3. Central Laboratory of 251 Hospital of PLA, Zhangjiakou 075000，China 4. Radiology Department, Wangjing Hosptial, China Academy of Chinese Medical Sciences, Beijing 100102, China
Abstract：In-situ analysis the chemical composition of tibial articular cartilage of female Hartley guinea pigs with Fourier transform infrared (FTIR) microspectroscopy was conducted. The infrared spectrum survey consists of three ages (1 months，2 months and 3 months)and three cartilage layers(surface, middle and deep). The results demonstrated that with ages increasing, the peak positions of main absorbance bands in surface and middle shifted to a lower wavenumber, and in deep they shifted to a lower wavenumber first, then shifted to a higher wavenumber. Infrared spectrum character of collagen, nucleic acid and proteoglycan were compared and analyzed, The ratios of I1 657/I1 548, I1 074/I1 548and I1 074/I1 237 tend to decrease with ages increasing in surface and middle. However, the ratios at 2 months are less than other ages in deep. These results are consistent with the regular pattern of cartilage ingredient change in different degradation stage, while the tibial platform images created by microscopic spectral imaging technology is highly compliant with pathology description. The authors’ primary result illustrated that FTIR microspectroscopy can be used for in-situ analysis of molecular constituents of different levels cartilages. The molecular information obtained from the study is important for understanding the pathogenesis of cartilage diseases.
杨丽平1，赵铁军2，刘建玲3，王金洪4，宋庆慧1，朱 嘉1，张万强1，孔焕宇1* . 豚鼠原发性骨关节炎的关节软骨显微傅里叶变换红外光谱研究[J]. 光谱学与光谱分析, 2012, 32(10): 2651-2655.
YANG Li-ping1, ZHAO Tie-jun2, LIU Jian-ling3, WANG Jin-hong4, SONG Qing-hui1, ZHU Jia1, ZHANG Wan-qiang1, KONG Huan-yu1* . Investigation of Articular Cartilage Using FTIR Microspectroscopy for Guinea Pig with Spontaneous Osteoarthritis . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2012, 32(10): 2651-2655.
[1] Bambery K R，Schultke E，Wood B R，et al. Biochim Biophys Acta, 2006，1758(7)：900. [2] Rubin S，Bonnier F，Sandt C，et al. Biopolymers，2008，89(2)：160. [3] Bendele A M, Human J F. Arthritis Rheum，1998，31(4)：561. [4] LAN Xu，LIU Xue-mei，GE Bao-feng, et al(蓝 旭，刘雪梅，葛宝丰，等). China Journal of Orthopaedics and Traumatology(中国骨伤)，2001，14(4)：212. [5] GAO Gai-xia，WEI Xiao-chun，LI Kai(高改霞，卫小春，李 凯). Journal of Clinical Rehabilitative Tissue Engineering Research(中国组织工程研究与临床康复)，2010, 14(24)：4385. [6] CHEN Bai-cheng，ZHANG Jing(陈百成，张 静). Osteoarthritis(骨关节炎). Beijing：People’s Medical Publishing House(北京：人民卫生出版社)，2004. [7] LU Sheng-jun，LIAO Quan-ming，LU Hou-gen(陆圣君，廖全明，鲁厚根). Int J Orthop.(国际骨科学杂志)，201l，32(3)：167. [8] LI Li-li，ZHAO Li-jiao，ZHONG Ru-gang(李莉莉，赵丽娇，钟儒刚). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2011，31(12)：3194. [9] SHA Hu，YANG Li(沙 湖，杨 莉). Practical Journal of Hospital Clinical(实用医院临床杂志)，2011，8(2)：180. [10] DAI Ying，HUANG Tao，BAI Xi-zhuang(戴 颖，黄 涛，白希壮). Foreign Medical Bone Volume of Science(国外医学·骨科学分册)，2004，25(2)：101. [11] XU Bin，LIU Tao，XU Hong-gang(徐 斌，刘 涛，徐洪港). Journal of Clinical Rehabilitative Tissue Engineering Research(中国组织工程研究与临床康复)，2010, 14(7)：1166. [12] WANG Jian-sheng，ZHANG Jia，WU Wen-an(王健生，张 佳，吴文安). Chemical Journal of Chinese Universites(高等学校化学学报)，2010，31(3)：484. [13] Susie Boydston-White，Melissa Romeo，Tatyana Chernenko，et al. Biochimica et Biophysica Acta，2006，1758(7)：908. [14] LING Xiao-feng，SHI Jing-sen，XU Yi-zhuang，et al(凌晓峰，石景森，徐怡庄，等). Chemical Journal of Chinese Universities(高等学校化学学报)，2007，28(3)：445. [15] Benedetti E，Teodori L, Trinca M L, et al. Applied Spectroscopy，l990，44(1)：276. [16] de Bri E, Jonsson K，Reinholt F P，et al. Acta Orthop Scand, 1996, 67(5)：498.
