1. 中国药科大学分析化学教研室，江苏 南京 210009 2. 药物质量与安全预警教育部重点实验室，江苏 南京 210009 3. Faculty of Pharmacy，University of Paris V;4 avenue de l’Observatoire，75006 Paris，France
Fluorescence Study on the Interactions between Carbon Nanotubes and Bovine Serum Albumin
LI Shan-shan1, HE Hua1, 2*, CHEN Zhe1, ZHA Jun1, Chuong Pham-Huy3
1. Division of Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, China 2. Key Laboratory of Drug Quality Control and Pharmacovigilance, China Pharmaceutical University, Nanjing 210009, China 3. Faculty of Pharmacy, University of Paris V, 4 avenue de l’Observatoire, 75006 Paris, France
Abstract：As a new family of nanomaterials, carbon nanotubes (CNTs) exhibit unique properties such as their capacity to penetrate into the cells and low immunogenicity, which have attracted increasing attention in biomedical field and make their application in drug and gene transfer systems being possible. So getting more information about the interaction of CNTs with biomacromolecules is crucial for further investigation of CNTs in therapeutic applications. The interaction between CNTs and BSA under physiological condition was investigated by fluorescence quenching, synchronous fluorescence and red edge excitation shift (REES)method. The results showed that CNTs could significantly decrease the fluorescence intensity of BSA. While the results of synchronous fluorescence and REES indicated that CNTs almost had no influence on the conformation of BSA. So the authors concluded that the mechanism of interaction between CNTs and BSA was non-specific adsorption.
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