微型生化分析系统实验测试

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摘要：介绍了一种基于微型光纤光谱仪的微型生化分析系统及其相应的标定和实验测试。实验中对总蛋白、白蛋白、双氯芬酸钠、亚硝酸盐、碘-淀粉、Fe²⁺-邻二氮菲等溶液体系的吸收谱进行了测试。根据不同浓度Fe²⁺-邻二氮菲溶液体系的实验结果，作出了相应的工作曲线，结果表明该工作曲线具有很好的线性度。实验结果与日本岛津分光光度计、美国海洋光学公司的HR2000微型光谱仪以及国产722型分光光度计的实验结果进行了比较。实验测试表明该微型生化分析系统已达到实用化的要求，且具有实时全光谱(360～800 nm)分析、体积小等优点，是一种比较理想的微型生化分析系统。

关键词：分析仪器;微系统;生化分析;吸收光谱

Abstract：A micro biochemical analytical system based on a micro fiber spectrometer is introduced. Experiment was carried out to calibrate and test the analysis system. In the experiment, the absorption spectra of Fe²⁺-ferroin solution bodies with different concentrations were obtained. The working curve shows a fine linearity of the analysis system. The authors also compared the experimental results obtained from 722-spectrometer and those from our analysis system. It was shown that their system can meet the requirement of practical use. This system also has many advantages, such as real-time whole spectrum analyzing and small volume, and is an ideal instrument for biochemical analysis.

Key words：Analytical instrument;Micro systems;Biochemical analysis;Absorption spectrum

收稿日期: 2003-10-16 修订日期: 2004-01-28

中图分类号:

TH83

通讯作者: 陈刚

引用本文:

陈刚¹，温志渝¹，温中泉¹，徐溢²，李霞¹，蒋子平¹ . 微型生化分析系统实验测试[J]. 光谱学与光谱分析, 2005, 25(03): 439-443.
CHEN Gang¹，WEN Zhi-yu¹，WEN Zhong-quan¹，XU Yi²，LI Xia¹，JIANG Zi-ping¹ . Experimental Testing of Micro Biochemical Analytical System . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(03): 439-443.

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https://www.gpxygpfx.com/CN/Y2005/V25/I03/439