Direct Determination of Ammonia in Plasma with Indophenol Method
DENG Jian1, XU Jin-sheng2, FAN Hua1, YUAN Ya-li1, LIU Chuan-xiang1
1. College of Chemistry and Chemical Engineering, Nanhua University, Hengyang 421001, China 2. Department of Chemistry, Hengyang Normal College, Hengyang 421008, China
Abstract：A new method for direct determination of ammonia in plasmas with indophenol reagent is described. It is shown that using sodium citrate as anticoagulant in phosphate buffer within pH 10.5-11.2, indophenol reagent reacts sensitively with ammonia in the plasmas to form a stable blue compound with a maximum absorption at 630 nm with low interference. The apparent molor absorptivity is 2.48×104 L·mol-1·cm-1，and the linear range 0-1 500 μmol·L-1 (r=0.999 9) with a detection limit of 1.7 μmol·L-1. The relative standard deviation is 1.13%(n=11) and the recoveries are in the range of 93.6%-101.6% with an average recovery of 97.0%. The proposed method has been applied to detect ammonia in the plasmas from 43 healthy persons and 97 patients who suffered from liver diseases with satisfactory results.
邓 健1，许金生2，樊 华1，袁亚莉1，刘传湘1 . 靛酚蓝显色法直接测定血浆氨[J]. 光谱学与光谱分析, 2005, 25(03): 436-438.
DENG Jian1, XU Jin-sheng2, FAN Hua1, YUAN Ya-li1, LIU Chuan-xiang1 . Direct Determination of Ammonia in Plasma with Indophenol Method . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(03): 436-438.
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