Fluorescence Study on the Interaction of Narrow Distribution Low Polymerization Degree Chitooligosaccharides and Human Serum Albumin
LIU Hai-kuan1, WANG Hai-yang1, WU Shan-shan1, ZHANG Qi1, 2*, DU Jin-feng1, GU Hai-bo1, 2
1. Key Laboratory of the National Ministry of Education for Tropical Biological Resources/Hainan Provincial Key Lab of Fine Chemistry, Hainan University, Haikou 570228, China 2. College of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China
Abstract：Chitosan (CTS), a linear binary copolymer of (1→8)-linked 2-acetamido-2-deoxy-β-D-glucopyranose (GlcNAc unit) and 2-amino-2-deoxy-β-D-glucopyranose (GlcNH2 unit), is derived from chitin by alkaline deacetylation. In the present work, the narrow molecular weight distribution chitooligosaccharides were prepared by degraded CTS with a microwave-assisted-cleavage method of metal-coordinating template-absorption catalytic oxidation. Under physiological pH conditions, the interaction of CTS and the narrow distribution chitooligosaccharides with Human serum albumin (HSA) was preliminarily explored by fluorescence spectra. Low molecular weight CTS in different concentration was added into HSA solution respectively, the absorptivity of the HSA solution decreased considerably. This phenomenon indicated that there was an interaction between these six different low molecular weight CTS with HSA, and the smaller the DP of the narrow distribution chitooligosaccharides, the stronger the interaction with HSA. However, the interaction gradually failed in as the DP was less than eight. The interaction almost disappeared when using glucosamine, the final product of degraded CTS, which revealed that there was a scale effect between chain-like CTS molecule and protein biomacromolecule. The results suggest that the strongest interaction binding occurs in CTS with DP≈8. Whether the DP increases or decreases, the interaction will weaken.
刘海宽1,王海洋1,吴姗姗1,张 岐1, 2*,杜金风1,顾海波1, 2 . 窄分子量分布低聚壳聚糖与HSA相互作用的荧光光谱研究[J]. 光谱学与光谱分析, 2009, 29(09): 2523-2526.
LIU Hai-kuan1, WANG Hai-yang1, WU Shan-shan1, ZHANG Qi1, 2*, DU Jin-feng1, GU Hai-bo1, 2 . Fluorescence Study on the Interaction of Narrow Distribution Low Polymerization Degree Chitooligosaccharides and Human Serum Albumin. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(09): 2523-2526.
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