Determination of Trace Free Copper and Zinc in Serum Samples by Graphite Furnace Atomic Absorption Spectrometry after Two-Step Precipitation of Protein
Abstract：A method was developed for the determination of trace free copper and zinc in serum sample by GFAAS after two-step precipitation. The serum and ethanol were mixed with volume ratio of 1∶2. The mixture was subsequently denatured at 70 ℃ and centrifuged to precipitate proteins. The determination.limit of copper (3σ) was 1.2 μg·L-1 and the recovery was 92.3%-104%, and the determination. Limit of zinc(3σ) was 0.098 μg·L-1 and the recovery was 90%-107%. This two-step precipitation method can be used to determine non-protein-bound copper in tumor patients and healthy people.
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