Abstract：4-nitrophenol (4-NP), a phenolic compound with highly toxic and carcinogenic properties, has become a global concern. As a fluorescence probe, a rapid and sensitive fluorescence assay for 4-NP was developed using cysteine-protected copper nanoclusters (Cys-CuNCs). The Cys-CuNCs were prepared using ascorbic acid-capped copper nanoparticles (CuNPs) as a precursor and cysteine as an etching agent. The prepared Cys-CuNCs showed excellent fluorescence properties and stability solubility with the maximum excitation and emission peak at 370 nm and 464 nm, respectively. In the presence of 4-NP, the fluorescence of CuNCs was quenched effectively. Single-factor experiments were investigated to optimize the pH of the buffer and the reaction time for nitrophenol detection. The optimal conditions were as follows: phosphate buffer (10 mmol·L-1, pH 8.0) as a working buffer, mixing 4-NP and CuNCs for fluorescence scan. A sensitive analysis method for nitrophenol was constructed by utilizing the variation of fluorescence intensity F0-F/F0 as abscissa and 4-NP concentration as ordinate. The fluorescence quenching rate of CuNCs showed a linear relationship with the concentration of 4-NP in the range from 1 to 100 μmol·L-1 with the detection limit of 0.31 μmol·L-1. Four other 4-NP homologues and eight other organics were selected as the interferent; all of them had almost no effect on the fluorescence of CuNCs, indicating that the method had good anti-interference ability. In addition, the strategy was successfully applied to detect 4-NP in lake water with satisfactory recoveries from 97.0%～101%. The relative standard deviation was 2.71%～3.60%, indicating that the method is accurate and could be used for 4-NP detection in actual samples.
何 璇，欧丽娟，谢钰丹，罗建新，杜 聪，张春燕. 半胱氨酸保护的荧光铜纳米簇快速检测对硝基苯酚[J]. 光谱学与光谱分析, 2024, 44(12): 3387-3390.
HE Xuan, OU Li-juan, XIE Yu-dan, LUO Jian-xin, DU Cong, ZHANG Chun-yan. The Cysteine-Protected Copper Nanoclusters for Rapid Detection of p-Nitrophenol. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(12): 3387-3390.
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