Study on the Correlation between PMI and OD Changes in Rat’s Plasma
LI Wei1,2,KE Yong1,HE Guang-she3,XU Yong-cheng1,WANG Zhen-yuan1*
1. Department of Forensic Medicine, Xi’an Jiaotong University, Xi’an 710061, China 2. Luoyang Railway Public Security Bureau, Luoyang 471002, China 3. Institute of Criminal Science of Shenmu, Yulin Public Security Bureau, Yulin 719300, China
Abstract：We chose the UV-Vis spectrophotometry as a new way to investigate the postmortem interval (PMI). One hundred fifty Sprague-Dawley female rats (weight 260 g±10 g, from Xi’an Jiaotong University Animal Center) were chosen and sacrificed by cervical dislocation. The bodies were kept in a controlled environmental chamber set at (20±2) ℃. The plasma was harvested in course of 0 to 24 hours after death. The optical density (OD) at different wavelengths was measured with an UV-Vis spectrophotometer (type-UV250). It was shown that the OD changes of plasma at 577, 416 and 275 nm in 24 hours were dramatically related to PMI, and the R-indexes were 0.969, 0.97 and 0.898. The regression formulae of these indexes were worked out taking OD as independent variable, and PMI as variable. The quadratic equations were: PMI=231.227ODplasma at wavelength of 577 nm-501.16OD２plasma at wavelength of 577 nm-3.080 9(R2=0.945)，PMI=31.7426ODplasma at wavelength of 416 nm-9.184 7OD2plasma at wavelength of 416 nm-3.183 7(R2=0.94)，and PMI=95.238 8ODplasma at wavelength of 275 nm-39.343OD2plasma at wavelength of 275 nm-32.408(R2=0.795). It was concluded that the OD changes of rat’s plasma are good and potential markers for the estimation of PMI and should be very useful in forensic practice.
Key words：Postmortem interval (PMI);Optical density (OD);Plasma
李伟1,2,柯咏1,贺光社3,徐永城1,王振原1* . 大鼠死后血浆吸光度变化与死亡时间关系的研究[J]. 光谱学与光谱分析, 2008, 28(12): 2944-2946.
LI Wei1,2,KE Yong1,HE Guang-she3,XU Yong-cheng1,WANG Zhen-yuan1* . Study on the Correlation between PMI and OD Changes in Rat’s Plasma. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(12): 2944-2946.
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