Abstract：The fluorescence characteristic of enrofloxacin-yttrium (Ⅲ) (ENRX-Y3+) was investigated in the present paper. Experimental results indicated that Y3+ ion could greatly increase the fluorescence intensity of the system. From this, a simple and sensitive method of determining the ENRX was set up. The fluorescence intensity of the system was measured with a 1 cm quartz cell at the excitation wavelengths of 274 nm and the emission wavelengths of 424 nm. The optimal conditions were obtained as follows: pH=5.5-6.2, 0.1 mol·L-1 hexamethylenamine (HMA) buffer, the concentration of yttrium(Ⅲ) was 1.0×10-4 mol·L-1. Under the optimal conditions, the fluorescence intensity of the system showed a good linear relationship with the concentration of ENRX in the range of 1.0×10-9-5.0×10-6 mol·L-1, its correlation coefficient was 0.998 1, and the detection limit (S/N=3) was 2.3×10-10 mol·L-1. Interferences of common metal ions and other pharmaceuticals usually used for the compatibility of medicines with the fluorescence intensity of the system were studied, and the results showed that most metal ions except Tb3+, Cu2+ and Al3+ and the compatible medicines used together with ENRX did not interfere with its determination. The recovery tests in the compatible medicines were also performed, and the results showed that all the recoveries were in the range of 98.0%-107.0%, and RSDs were in the range of 0.9%-4.5%. The presented method was used to determine the ENRX in actual pharmaceutical samples which were used in the treatment for animals as compared with the reported method of terbium (Ⅲ) luminescence probe, and the results were satisfactory. In addition, the luminescence mechanism of the ENRX-Y3+ system was also discussed.
[1] JIAN Ji-chang, WU Zao-he, CHEN Gang(简纪常，吴灶和，陈 刚). Chinese Journal of Veterinary Science(中国兽医学报)，2005，25(2): 195. [2] ZHONG Xiao-ning, ZHANG Xuan, GONG Xiang-lian(仲晓宁，张 璇，巩相莲). Chinese Journal of Veterinary Drug(中国兽药杂志)，2005，39(9): 26. [3] Espinosa-Mansilla A, Muoz de la Pea A, González Gómez D, et al. J. Chromatogr. B, 2005, 822(1-2): 185. [4] Liang H R, Kays M B, Sowinski K M. J. Chromatogr. B, 2002, 772(2): 53. [5] LIANG Yao-dong, LI Jian-zhong, ZHANG Zhu-jun(梁耀东，李建中，章竹君). Chinese Journal of Analytical Chemistry(分析化学)，1997，25(11): 1307. [6] LIU Hong, ZENG Zhen-ling, YANG Gui-xiang, et al(刘 红，曾振灵，杨桂香，等). Chinese Journal of Veterinary Drug(中国兽药杂志)，2006，40(11): 13. [7] ZHANG Guo-wen, NI Yong-nian(张国文，倪永年). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2006，26(1): 113. [8] WANG Jing-ping, DU Li-ming, XU Qing-qin(王静萍，杜黎明，许庆琴). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2002，22(2): 287. [9] TONG Chang-lun, XIANG Guang-hong, HUANG Di-jin, et al(童裳伦，项光宏，黄迪金，等). Chinese Journal of Analytical Chemistry(分析化学)，2004，32(5): 619. [10] TONG Chang-lun, XIANG Guang-hong, LIU Wei-ping(童裳伦，项光宏，刘维屏). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2005，25(12): 2061. [11] Han Y X, Wu X, Yang J H, et al. J. Pharmaceut. Biomed. Anal., 2005, 38: 528. [12] Tong C L, Xiang G H. Journal of Fluorescence, 2006, 16(6): 831. [13] WANG Shao-ning, DENG Yi-hui, CHEN Yong, et aL(王绍宁，邓意辉，陈 咏，等). Chinese Journal of Pharmaceutical Analysis(药物分析杂志)，2003，23(3): 192. [14] DU Li-ming, JIN Wei-jun, DONG Chun, et al(杜黎明，晋卫军，董 川，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2001，21(4): 518. [15] Tong C L, Xiang G H. J. Lumin., 2007, 126(2): 575. [16] Djurdjevic P T, Jelikic-Stankov M, Stankov D. Anal. Chim. Acta, 1995, 300(1-3): 253. [17] Yang J H, Tong C L, Jie N Q, et al. J. Pharmaceut. Biomed. Anal., 1997, 15: 1833.
