三种烟草品种基因组DNA傅里叶红外光谱研究

doi:10.3964/j.issn.1000-0593(2014)04-0967-05

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摘要：利用傅里叶红外光谱(FTIR)研究三种烟草品种DNA的差异，旨在对烟草品种进行亲缘关系分析和品种鉴定。研究结果显示，三种烟草品种DNA 红外光谱较为相似，均有四个明显的特征峰，1 103 cm^-1是DNA磷酸二酯键的对称伸缩振动，1 236 cm^-1是DNA 磷酸二酯键的非对称伸缩振动，1 400 cm^-1 是DNA糖苷键的伸缩振动，1 622 cm^-1是DNA中是胞嘧啶C4—C5=C6的环伸缩振动。通过平滑、标准化处理、二阶求导、提取主成分和系统聚类分析建立了DNA FTIR光谱数据聚类分析模型。使用该模型对三个烟草品种进行鉴定，鉴定正确率为100%。使用该模型对三个烟草品种进行亲缘关系分析，云烟87与K326聚为一类，距离系数为0.003，DNA相似度为99.7%，红大单独聚为一类。聚类正确率达100%。该项研究为烟草品种鉴定及遗传育种提供了参考。

关键词：傅里叶红外光谱(FTIR);烟草;基因组 DNA;主成分分析;系统聚类;亲缘关系

Abstract：The aim is analyzing genetic reLationship and identifying varieties by detecting DNA differences of three kinds of Nicotiana tabacum L. using Fourier infrared spectrum (FTIR). Results show that DNA FTIR of three kinds of Nicotiana tabacum L. is relatively similar. They all have four obvious characteristic peaks. 1 105 cm^-1 beLongs to symmetrical stretching vibration of phosphodiester bond，1 250 cm^-1 is unsymmetrical stretching vibration of phosphodiester bond, 1 400 cm^-1 is contributed to glucosidic bond, and 1 622 cm^-1 belongs to C4C5C6 stretching vibration of cytosine. DNA FTIR data was handled by smoothing, standardizing, second derivative, principal component analysis and Hierarchical cluster analysis. The standard model of Hierarchical cluster combined with principal component of the second derivative was set up. The correct rate of identification is 100%. Yunyan 87 and K326 were clustered into one by using the model. The distance coefficient is 0.003, and DNA similarity is 99.7%, Hongda was clustered into one by itself. The correct rate of cluster is 100%. The study provides a reference for Nicotiana tabacum L. variety identification and genetic breeding.

Key words：Fourier infrared spectrum;Nicotiana tabacum L.;Genomic DNA;Principal component analysis;Hierarchical cluster analysis;Genitic elationship

收稿日期: 2013-09-16 修订日期: 2014-01-10

中图分类号:

O657.3

通讯作者: 邱璐 E-mail: qiulu@cxtc.edu.cn

引用本文:

邱璐¹，杨海艳¹，杨春胜¹，刘鹏²，范树国¹，刘仁明³，周林宗⁴ . 三种烟草品种基因组DNA傅里叶红外光谱研究 [J]. 光谱学与光谱分析, 2014, 34(04): 967-971.
QIU Lu¹, YANG Hai-yan¹, YANG Chun-sheng¹, LIU Peng², FAN Shu-guo¹, LIU Ren-ming³, ZHOU Lin-zong⁴ . Study on DNA Fourier Infrared Spectroscopy of Three Kinds of Nicotiana Tabacum L. . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2014, 34(04): 967-971.

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