Determination of 8 Trace Elements in Mantle Muscle and Cuttlebone of Sepiella maindroni by ICP-MS Using Microwave Digestion for Sample Preparation
WU Chang-wen1, 2, CHI Chang-feng2, HE Guang-yuan1, XU Mei-ying2
1. Key Laboratory of Molecular Biophysics, Ministry of Education and China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, Huazhong University of Science and Technology, Wuhan 430074, China 2. Laboratory of Marine Culture Equipments and Engineering Technique, School of Marine Science, Zhejiang Ocean University, Zhoushan 316004, China
Abstract：An inductively coupled plasma mass spectrometry (ICP-MS) for determi-nation of the contents of 8 trace elements in mantle muscle and cuttlebone of Sepiella maindroni after microwave digestion of the sample has been developed. Satisfactory linearity of working curves for the 8 elements was obtained, giving all their correlation coefficients over 0.997 3. The precision of measurement ranges from 2.4% to 8.7% in terms of relative standard deviation. The recoveries and the limits of detection are in the range of 96.5%-106.3% and 0.002-0.032 μg·L-1, respectively. It was indicated that the proposed method had the advantages of simplicity, speediness and sensitivity. The results showed that the mantle muscle and cuttlebone of Sepiella maindroni contained rich trace elements Zn and Cu, but the contents of Cd and As are higher than the limits of Chinese Pharmacopoeia, Green Trade Standard for Importing and Exporting Medicinal Plant and Preparation and U.S. Food and Drug Standard. Furthermore, our study provides new scientific foundation for the quality control, culture, general application, resource utilization and exporting of Sepiella maindroni.
Key words：Sepiella maindroni;Trace elements;Inductively coupled plasma mass spectrometry (ICP-MS);Microwave digestion
吴常文1, 2，迟长凤2，何光源1，徐梅英2 . 微波消解ICP-MS法测定曼氏无针乌贼肉和海螵蛸中八种微量元素[J]. 光谱学与光谱分析, 2009, 29(12): 3395-3398.
WU Chang-wen1, 2, CHI Chang-feng2, HE Guang-yuan1, XU Mei-ying2 . Determination of 8 Trace Elements in Mantle Muscle and Cuttlebone of Sepiella maindroni by ICP-MS Using Microwave Digestion for Sample Preparation . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(12): 3395-3398.
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