Prediction of Leaf Concentration in Alfalfa Using Near Infrared Reflectance Spectroscopy
NIE Zhi-dong1,HAN Jian-guo1*,YU Zhu1,ZHANG Lu-da2
1. Institute of Grassland Science, China Agricultural University, Beijing 100094, China 2. College of Science, China Agricultural University, Beijing 100094, China
Abstract：Leaf concentration in alfalfa is an important factor affecting the nutritive value, forage intake and digestibility. Estimates of leaf concentrations commonly used currently involve a labor intensive process of hand separating leaf and stem fractions. In the present study, a total of 41 artificial alfalfa samples were mixed with different leaf concentrations ranging from 15% to 55%. The object was to develop 3 calibrations for predicting alfalfa leaf concentrations using 15,25 and 35 calibrated samples by near infrared reflectance spectroscopy. The root mean square error of prediction(RMSEP)was 1.02, 1.97 and 0.51, respectively. External validation had a coefficient of determination (r2) ranging from 0.979 8 to 0.998 9. The ratio of performance to standard deviation (RPD) varied from 2.85 to 25.93.The results showed that 15 samples could develop accurate NIRS model of alfalfa leaf concentrations；the calibration equations got better accuracy with the increase in calibrated samples numbers from 15 to 35.
聂志东1,韩建国1*,玉柱1,张录达2. 近红外光谱法测定苜蓿中的叶含量[J]. 光谱学与光谱分析, 2008, 28(02): 317-320.
NIE Zhi-dong1,HAN Jian-guo1*,YU Zhu1,ZHANG Lu-da2. Prediction of Leaf Concentration in Alfalfa Using Near Infrared Reflectance Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2008, 28(02): 317-320.
[1] XU Guang-tong, YUAN Hong-fu, LU Wan-zhen(徐广通，袁洪福，陆婉珍). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2000,20(2): 134. [2] Norris K H, Barnes R F, Moore J E, et al. J. Anim. Sci., 1976, 43(4): 889. [3] Brown W F, Moore J E, Kunkle W E, et al. J. Anim. Sci., 1990, 68(5): 1416. [4] Coleman S W, Murray I. Anim. Feed Sci. Tech., 1993, 44(3): 237. [5] NIE Zhi-dong, HAN Jian-guo, YU Zhu, et al(聂志东，韩建国，玉 柱，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2007，27(7)：1308. [6] LIU Xian, HAN Lu-jia(刘 贤，韩鲁佳). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2006，26(11)：2016. [7] Pitman W D, Piacitelli C K, Aiken G E, et al. Agron. J., 1991, 83(1): 103. [8] Coleman S W, Christiansen S, Shenk J S. Crop Sci., 1990, 30(1): 202. [9] Volesky J D, Coleman S W. J. Range Manage., 1996, 49(2): 163. [10] Locher F, Heuwinkel H, Gutser R, et al. Agron. J.,2005, 97(1): 11. [11] Locher F, Heuwinkel H, Gutser R, et al. Agron. J.,2005, 97(1): 18. [12] Hill N S, Perterson J C, Studemann J A, et al. Crop Sci., 1988,28(2): 354. [13] Smart A J, Schacht W H, Pedersen J F, et al. J. Range Manage., 1998, 51(4): 447. [14] Garcia-Criado B, Garcia-Criado A, Perez-Corona M E. J. Sci. Food Agic., 1991, 57(4): 507. [15] Williams P C. Near Infrared Technology in the Agricultural and Food Industries, American Association of Cereal Chemist, Minnesota, USA：St. Paul,2001. 164.
