Effect of Aggregation Extent of Microperoxidase-8 on Its Interaction with La3+
CHEN Ting-ting1, HUANG Xiao-hua1, DU Jiang-yan1, FENG Yu-ying1, LIU Chang-peng2, XING Wei1, LU Tian-hong1, 2*
1.College of Chemistry and Environment Science, Nanjing Normal University,Nanjing 210097, China 2.Changchun Institute of Applied Chemistry, Chinese Academy of Sciences,Changchun 130022, China
Abstract:The results of UV-Vis absorption spectroscopic investigation indicated that La3+ coordinates strongly with carboxylate oxygen of two propionic acid groups of the heme group in an MP-8 molecule, leading to an increase in the non-planarity of the porphyrin ring of the heme group.The increasing extent in the non-planarity of the porphyrin ring of the heme group due to the interaction of La3+ and MP-8 molecule would be significantly decreased because NaCl could increase the aggregation extent of MP-8 molecules.Although methanol can reduce the aggregation extent of MP-8 molecules, it has little influence on the increasing extent in the non-planarity of the porphyrin ring of the heme group due to the interaction of La3+ and MP-8 molecule.
陈婷婷1,黄晓华1,杜江燕1,冯玉英1,刘长鹏2,邢 巍2,陆天虹1, 2* . 微过氧化物酶-8的聚集程度对其与La3+相互作用的影响[J]. 光谱学与光谱分析, 2005, 25(08): 1270-1273.
CHEN Ting-ting1, HUANG Xiao-hua1, DU Jiang-yan1, FENG Yu-ying1, LIU Chang-peng2, XING Wei1, LU Tian-hong1, 2* . Effect of Aggregation Extent of Microperoxidase-8 on Its Interaction with La3+ . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(08): 1270-1273.
[1] Adams P A, Goold R D.J.Chem.Soc.Chem.Commun., 1990, 97. [2] Osman A M, Koerts J, Boersma M G, et al.Eur.J.Biochem., 1996, 240: 232. [3] Melchionna S, Barteri M, Ciccotti G.J.Phys.Chem., 1996, 100: 19241. [4] Aron J, Baldwin D A, Marques H M.J.Inorg.Biochem., 1986, 27: 227. [5] Aron J, Baldwin D A, Marques H M, et al.J.Inorg.Biochem., 1986, 27: 227. [6] Urry D W, Pettegrew J W.J.Am.Chem.Soc., 1967, 89: 5276. [7] Blumberg W E, Peisach J.J.Biol.Chem., 1965, 240: 870. [8] Shelmutt J A.Dobry M M, Satterlee J D.J.Phys.Chem., 1984, 88: 4890. [9] CHEN Ting-ting, HUANG Xiao-hua,FENG Yu-ying, DU Jiang-yan,LIU Chang-peng,XING Wei,LU Tian-hong(陈婷婷, 黄晓华, 冯玉英, 杜江燕, 刘长鹏, 邢 巍, 陆天虹).Chinese J.Inorg.Chem.(无机化学学报), 2003,9: 941. [10] Harbury H A, Loach P A.J.Biol.Chem., 1960, 235: 3640. [11] Medforth C J, Senge M O, Smith K M, et al.J.Am.Chem.Soc., 1992, 114: 9859. [12] Shelnutt J A, Medforth C J, Berber M D, et al.J.Am.Chem.Soc., 1991, 113: 4077. [13] LI Xiao-jing, WANG Zhi-qiang, CHEN Ji, et al(李晓晶, 王志强, 陈 继,等).Chinese J.Applied Chem.(应用化学), 1998, 15: 5.