Abstract：The fluorescence and synchronous fluorescence spectra were employed to study the binding between sodium azide(NaN3) and horseradish peroxidase (HRP), which was affected by the molecular conformation and the microenvironment of the fluorescence residues. The mechanism of the fluorescence quenching of HRP by NaN3 was discussed and the binding constant and the number of binding sites of non-covalent bond between them were calculated.
夏炳乐，彭敦耕，牛海霞，刘清亮 . 叠氮化钠对辣根过氧化物酶的荧光猝灭机制的研究[J]. 光谱学与光谱分析, 2005, 25(03): 412-415.
XIA Bing-le, PENG Dun-geng, NIU Hai-xia, LIU Qing-liang . Research on the Mechanism of Fluorescence Quenching of HRP by NaN3 . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(03): 412-415.
[1] Marc B, Marc A. J. Am. Chem. Soc., 1997, 119: 576. [2] Haesun K, Harold E. Biochemistry, 1989, 28: 5714. [3] Gaspar S, Habermuller K, Csoregi E. Sensors and Actuators:B, 2001, 72: 63. [4] Liu J Zh, Song H Y, Weng L P et al. Journal of Molecular Catalysis B: Enzymatic，2002，18：225. [5] Mester T, Tien M. International Biodeterioration & Biodegradation，2000，46：51. [6] Otiz De Montellano, P R, David S K. Ator M A, Tew D. Biochemistry，1988，27(15)：5470. [7] Tatarko Matthew, Bumpus A John. Archives of Biochemistry and Biophysics，1997, 339: 200. [8] CＨＥＮ Zeng-xie, LＩＵ Ｊing, LＵＯ Dan(陈曾燮，刘 兢，罗 丹). Biochemical Experiment(生物化学实验). Hefei：The Press of University of Science and Technology of China(合肥：中国科学技术大学出版社)，1994. 2７９. [9] JＩＡＮＧ Tai-jiao, JＩＥ Xin-song, YＵＡＮ Zhong-yi(蒋太交，吉鑫松，袁中一). Acta Biochimica and Biophysica Sinica(生物化学与生物物理学报), 1998，30: 132. [10] DＵ Xiu-lian, LＩ Rong-chang, WＡＮＧ Kui(杜秀莲，李荣昌，王 夔). Chinese Science Bulletin(科学通报)，2001，46：394. [11] Lakavicz J R, Weber G. Biochemistry, 1973, 12: 4161. [12] LＵ Ji-xin(卢继新). Acta Chimica Sinica(化学学报)，1997，55: 915. [13] Mauricio S B, Guilherme L I. J. Phys. Chem., 1998, 102(23): 4678. [14] Abert W C, Gregory W M, Allan G S. Anal. Biochem., 1993, 213: 407. [15] SＨEＮＧ Liang-quan, LＩＵ Shao-min, YＥ Ji-hua(盛良全，刘少民，叶纪华). Chinese Journal of Inorganic Chemistry(无机化学学报)，2003, 19：191. [16] XＩＡＯ Hou-rong, SＨＩ Chun-hua, XＩＡ Bing-le et al(肖厚荣，施春华，夏炳乐等). Chinese Journal of Inorganic Chemistry(无机化学学报)，2003, 19：589.
