Abstract：In 0.01 mol·L-1 Hepes, pH 7.4 and at room temperature the binding of HBED with Gd(Ⅲ) or Yb(Ⅲ) was monitored by UV difference spectrum. The results show that the molar ratio of the complexes is most likely 1∶1. Gd-HBED or Yb-HBED complex produced peaks at 237 nm and 291 nm. The molar absorptivities of Gd-HBED and Yb-HBED at 237 nm are ΔεGd= (22.52±0.20)×103 cm-1·mol-1·L, ΔεYb=(27.15±0.11)×103 cm-1·mol-1·L, respectively. The conditional equilibrium constants for the complexes were measured to be lgKGd-HBED=13.56±0.28 and lgKYb-HBED=16.06±0.03. A linear free energy relationship for the complexes of Yb(Ⅲ) and Gd(Ⅲ) has been established by using equilibrium data on 18 complexes. The HBED binding constants for Yb(Ⅲ) and Gd (Ⅲ) are in agreement with the linear free energy relationship.
王金铃1，毕和平2，杨斌盛1* . 紫外差光谱测定Gd(Ⅲ)，Yb(Ⅲ)与HBED配合物的条件稳定常数 [J]. 光谱学与光谱分析, 2005, 25(01): 89-91.
WANG Jin-ling1，BI He-ping2，YANG Bin-sheng1* . Determination on Conditional Equilibrium Constants of the Complexes of Gd(Ⅲ) and Yb(Ⅲ) with HBED by UV Difference Spectra . SPECTROSCOPY AND SPECTRAL ANALYSIS, 2005, 25(01): 89-91.
[1] Harris W R，Carrano C J，Pecoraro V L，Raymond K N，J. Am. Chem. Soc., 1981, 103：2231. [2] Li H，Sadler P J，Sun H. Eur. J. Biochem.，1996, 242：387. [3] Aison P, Leibman A, Zweier J. J. Biol. Chem., 1978, 253：1930. [4] Kurokawa H, Mikami B, Hirose M. J. Mol. Biol., 1995, 254：196. [5] YANG Bin-sheng, LI Ying-qi(杨斌盛，李英奇). Chemical Journal of Chinese Universities(高等学校化学学报)，2001，22(1)：1086. [6] ZHANG Ai-ping, YANG Bin-sheng(张爱萍，杨斌盛). Chemical Journal of Chinese Universities(高等学校化学学报)，1999，20(7)，1106. [7] L'Eplattenier F, Murase I, Martell A E. J. Am. Chem. Soc., 1967, 89：837. [8] BAI Hai-jing, LIU Wen, YANG Bin-sheng(白海静，刘 文，杨斌盛). Chinese Journal Inorganic Chemistry(无机化学学报)，2001，17(3): 389. [9] LI Ying-qi, BAI Hai-jing, YANG Bin-sheng(李英奇, 白海静，杨斌盛). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2002，22(3)：433. [10] Marlell A E, Smith E M. Critical Stability Constants. New York: 1974. 112.
