Analysis of Mid-Infrared Spectral Characteristics of Soils Cultivated With Salvia Miltiorrhiza at Different Intervals Based on Infrared Spectroscopy
QIAO Lu1, 2, LIU Yong-hong1, 2, XU Ke-ke1, 2, YU Huan-ying1, CHEN Yuan-jie3, YANG Lin-lin1, 2, DONG Cheng-ming1, 2*, WANG Lei1*
1. School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China
2. Henan Provincial Ecological Planting Engineering Technology Research Center of Authentic Medicinal Materials, Zhengzhou 450046, China
3. School of Pharmacy, Henan University, Kaifeng 475000, China
Abstract：The continuous cropping obstacle caused by the differential changes in beneficial and pathogenic bacteria in the rhizosphere mediated by root exudates is the main factor restricting the development of Salvia miltiorrhiza cultivation. Propose a method based on infrared spectroscopy technology combined with two-dimensional correlation spectroscopy to quickly detect the composition and content changes of root exudates of Salvia miltiorrhiza. The spectra of 7 kinds of Salvia miltiorrhiza soil samples in the same area (not planted with Salvia miltiorrhizae), 1 year of rotation (19-ED, 23-ED), 2 years of rotation (23-One), 3 years of rotation (23-Two), 5 years of rotation (19-Five), and Salvia miltiorrhiza (19-ING) were collected, and the characteristics of the extraction were analyzed. The composition of soil compounds was analyzed. The results showed that the infrared spectral peaks and peak shapes of salvia miltiorrhiza cultivated at different intervals were the same. The main characteristic absorption peaks were around 3 622, 3 380, 1 638, 995, 777, 693, 524 and 463 cm-1, respectively, to characterize the functional groups of phenolic hydroxy —OH, carbonyl C═O, methylene, benzene ring absorption substitution, cyclic ketones and other substances in phenolic acids. The absorbance of the soil with 1 year of rotation was the strongest at each characteristic peak, indicating that the autotoxic substances such as phenolic acids and esters continued to accumulate during the planting process. The position, number, and color of the absorption peaks were different in the bands 3 750~3 600, 2 170~2 145, 2 060~2 030, and 530~585 cm-1, which clearly characterized the differences in functional groups. This indicates that the combination of infrared and 2D correlation spectroscopy can rapidly detect and monitor organic compounds in soil, and provide a theoretical basis for exploring the formation and reduction mechanisms of continuous cropping obstacles in Salvia miltiorrhiza.
乔 璐，刘永红，徐可可，余焕英，陈园杰，杨林林，董诚明，王 磊. 基于红外光谱技术对不同间隔年限栽培丹参土壤的红外光谱特征分析[J]. 光谱学与光谱分析, 2025, 45(02): 483-491.
QIAO Lu, LIU Yong-hong, XU Ke-ke, YU Huan-ying, CHEN Yuan-jie, YANG Lin-lin, DONG Cheng-ming, WANG Lei. Analysis of Mid-Infrared Spectral Characteristics of Soils Cultivated With Salvia Miltiorrhiza at Different Intervals Based on Infrared Spectroscopy. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2025, 45(02): 483-491.
[1] Chinese Pharmacopoeia Commission(国家药典委员会). Pharmacopoeia of the People's Republic of China(中华人民共和国药典)，Part One(第一部). Beijing: China Medical Science and Technology Press(北京：中国医药科技出版社), 2020. 76.
[2] YANG Bai-yu, MIAO Yan-ping, FAN Xiao-qing, et al(杨白玉, 苗艳萍, 范晓青). Journal of Changchun University of Chinese Medicine(长春中医药大学学报), 2013, 29(6): 1121.
[3] DONG Shuai, WANG Hui, XIE Zhi-shen，et al(董 帅, 王 辉, 谢治深). Journal of Liaoning University of Traditional Chinese Medicine(辽宁中医药大学学报), 2019, 21(11): 152.
[4] WANG Miao, QIAO Li, XU Kuo，et al(王 苗, 乔 利, 绪 扩, 等). World Science and Technology—Modernization of Traditional Chinese Medicine and Materia Medica(世界科学技术—中医药现代化), 2022, 24(11): 4167.
[5] WANG Wei-wei, BU Xian-zhong, YANG Han-li, et al(王微微, 卜献忠，杨汉立, 等). Journal of Liaoning University of Traditional Chinese Medicine(辽宁中医药大学学报), 2023, 25(8): 54.
[6] LEI Li, GUO Qiao-sheng, WANG Chang-lin, et al(雷 丽, 郭巧生, 王长林, 等). China Journal of Chinese Materia Medica(中国中药杂志), 2018, 43(9): 1818.
[7] CUI Ning, CHEN Wei-xu, LI Qi, et al(崔 宁, 陈为序, 李 琦, 等). Modern Chinese Medicine(中国现代中药)，2021, 23(9): 1595.
[8] HUANG Ju-ying, CUI Dong, LIU Yu-shan, et al(黄菊英, 崔 东, 刘玉珊, 等). Jiangsu Agricultural Sciences(江苏农业科学), 2023, 51(3): 8.
[9] JIAO Huan-ran, MENG Yuan, ZHOU Bing-qian, et al(焦焕然, 孟 缘, 周冰谦, 等). Journal of Chinese Medicinal Materials(中药材), 2022, 45(7): 1538.
[10] DENG Jing, DU Chang-wen, ZHOU Jian-min, et al(邓 晶, 杜昌文, 周健民, 等). Soils(土壤), 2008, 40(6): 872.
[11] QIAO Lu, LIU Rui-na, ZHANG Rui(乔 璐, 刘瑞娜, 张 瑞, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2023, 43(2): 541.
[12] LIU Yao-lin(刘耀林). Geographic Information System(地理信息系统). Beijing: China Agriculture Press(北京：中国农业出版社), 2011: 237.
[13] WANG Feng-ling, ZHOU Jian-ke, WU Jing，et al(王凤岭, 周建科, 吴 婧，等). Modern Instruments(现代仪器), 2006，(5): 18.
[14] PEI Yue-hu(裴月湖). Spectral Analysis of Organic Compounds(有机化合物波谱解析). Beijing: China Medical Science and Technology Press(北京：中国医药科技出版社), 2019: 28.
[15] XIA Zhi-chao, SU Dan, YANG Jing-yan(夏智超, 苏 丹, 杨璟嬿). Market Modernization(商场现代化), 2022，(12): 86.
