1. 中国药科大学分析化学教研室，江苏 南京 210009 2. 中国药科大学有机化学教研室，江苏 南京 210009 3. Faculty of Pharmacy，University of Paris V, 4 Avenue de l’Observatoire，75006 Paris，France 4. 中国药科大学药物质量与安全预警教育部重点实验室，江苏 南京 210009
Fluorescence Study on the Interactions between G3.0 PAMAM Dendrimers and Bovine Serum Albumin
HE Hua1, 4, LI Shan-shan1, LU Jin-rong2, GU Yan1, Chuong Pham-Huy3
1. Division of Analytical Chemistry，China Pharmaceutical University，Nanjing 210009, China 2. Division of Organic Chemistry，China Pharmaceutical University，Nanjing 210009, China 3. Faculty of Pharmacy, University of Paris V, 4 Avenue de l’Observatoire, 75006 Paris, France 4. Key Laboratory of Drug Quality Control and Pharmacovigilance，Ministry of Education，China Pharmaceutical University，Nanjing 210009, China
Abstract：The interaction between amine terminated G3.0 PAMAM dendrimers and bovine serum albumin (BSA) under physiological condition was studied by fluorescence spectroscopy. Our experiments demonstrated that the fluorescence intensity of BSA decreased after the addition of G3.0 PAMAM dendrimers and the quenching mechanism was suggested as static quenching according to the Stern-Volmer equation. The binding constant of G3.0 PAMAM dendrimers with BSA was calculated to be 1.067±0.025 L·mmol-1. At the same time, synchronous fluorescence and red edge excitation shift(REES)were adopted to review the conformational changes of BSA influenced by G3.0 PAMAM dendrimers, which provides important significance for clinical medication. And the results indicated that G3.0 PAMAM dendrimers can change the conformation of BSA. Furthermore, this article also examined the influence of pH and ionic strength on the interactions, from which we can conclude that electrostatic interaction played major roles in the binding process. In conclusion, the fluorescence method is a highly sensitive and convenient way to study intermolecular interaction. Further investigation in this field will provide more important information for understanding the pharmacological effects and toxicities of drugs in human body.
何 华1, 4, 李杉杉1,芦金荣2,顾 艳1,Chuong Pham-Huy3 . 荧光光谱法研究G3.0 PAMAM树状大分子与牛血清白蛋白的相互作用[J]. 光谱学与光谱分析, 2009, 29(10): 2782-2786.
HE Hua1, 4, LI Shan-shan1, LU Jin-rong2, GU Yan1, Chuong Pham-Huy3 . Fluorescence Study on the Interactions between G3.0 PAMAM Dendrimers and Bovine Serum Albumin. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(10): 2782-2786.
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