不同品种大豆耐铝性的FTIR分析研究

doi:10.3964/j.issn.1000-0593(2009)02-0372-06

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Abstract The spectra of root, stem, leaf of soybean samples with 0, 20, 40, 60 mg·L^-1 Al³⁺ were determined by Fourier transform infrared (FTIR) spectrometry with OMNI-sampler. Little difference was found in the spectra of leaf between two soybean cultivars, aluminum-resistant cultivar Zhechun NO.2 and aluminum-sensitive cultivar Zhechun NO.3, except the indices of wave number-absorbance from 928 to 1 200 cm^-1, and similar results were also observed in stem and root samples of the two soybean cultivars with 0 mg·L^-1 Al³⁺. However, results from the comparison of the spectra showed some distinguishable differences in the intensity and the shape of absorption peaks of their FTIR spectra from 721 to 3 366 cm^-1 of Al-stressed samples and control samples between the two soybean cultivars, and more evident differences of FTIR were exhibited in Al-stressed roots, stems and leaves with higher concentration of Al³⁺. The increased absorbance at 2 929 and 3 350 cm^-1 was found in root FTIR spectra with 20, 40, 60 mg·L^-1 Al³⁺, while roots got maximum absorbance at wave number of 1 375 cm^-1 with 20 mg·L^-1 Al³⁺, which decreased with higher concentration of Al³⁺, and the same results were showed at wave numbers of 1 410, 1 423, 1 549 and 1 645 cm^-1. Absorption peak showed maximum at wave numbers of 1 051, 2 850, 2 929 and 3 350 cm^-1 in stem FTIR spectra with 60 mg·L^-1 Al³⁺. There was little difference between the spectra of Al-stressed leaves and controls at wave numbers from 1 750 to 2 750 cm^-1, but visible difference in leaf spectra was exhibited at other wave number. Moreover, the results showed that the FTIR spectra of aluminum-sensitive cultivar Zhechun NO.3 showed much more observable differences than aluminum-resistant cultivar Zhechun NO.2 with different concentration of Al, implying that the material metaboly of aluminum-sensitive soybean was evidently affected by Al. Therefore, FTIR spectra could be used broadly for identification of the endurance of different soybean cultivars to Al.

Key words：Fourier transform infrared spectroscopy;Soybean;Endurance to Al

Received: 2007-09-28 Accepted: 2007-12-29

ZTFLH:

S565.1

Corresponding Authors: LIU Peng E-mail: sky79@zjnu.cn

Cite this article:

ZHANG Zheng-yan,LIU Peng,CHEN Wei-wei, et al. FTIR Study of the Endurance to Al of Different Soybean Cultivars[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2009, 29(02): 372-377.

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https://www.gpxygpfx.com/EN/10.3964/j.issn.1000-0593(2009)02-0372-06 OR https://www.gpxygpfx.com/EN/Y2009/V29/I02/372

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