|
|
|
|
|
|
| In Situ Investigation of the Interactions of Pyrene and Phenanthrene with Humic Acid Using Laser Induced Nanoseconds Time Resolved Fluorescence Quenching Method Combined with PARAFAC Analysis |
| YANG Cheng-hu1, LIU Yang-zhi1, ZHU Ya-xian2, ZHANG Yong1，3* |
1. State Key Laboratory of Marine Environmental Science of China (Xiamen University), College of the Environment and Ecology, Xiamen University, Xiamen 361102, China
2. College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
3. Zhangzhou Institute of Technology, Zhangzhou 363000, China |
|
|
|
|
Abstract The fluorescence quenching method using laser-induced nanosecond time-resolved fluorescence (LITRF) combined with parallel factor (PARAFAC) analysis was developed for in situ investigation of the interactions of two typical polycyclic aromatic hydrocarbons (PAHs), pyrene (Pyr) and phenanthrene (Phe) either singly or in a mixture with Aldrich humic acid (HA). The concentration of free Pyr and Phe cannot be determined directly at the same time because the LITRF spectra of Pyr, Phe and HA are overlapping at the excitation wavelength of 266 nm. The fluorescent interference of HA can be eliminated quickly and effectively, while the fluorescence intensity and fluorescence decay curves of Pyr and Phe can be acquired by LITRF-PARAFAC analysis. The binding characteristics of PAHs with HA were described with Freundlich isothermal model. Nonlinear binding (n<1) and competitive sorption of Pyr and Phe on HA were verified, which showed that the linearity increased (n tend to be 1) and the single point binding coefficient decreased after the addition of Pyr or Phe. The degree of competition was depended on the concentrations of cosolutes, and competition was much stronger in the case of relatively high competitor concentrations. In addition, the results indicated that the binding characteristics of single component Pyr and Phe with HA obtained with LITRF-PARAFAC quenching method were in accordance with conventional fluorescence quenching method. The quenching mechanism for Pyr and Phe by HA was primarily static quenching which was also verified by quenching rate constant and fluorescence lifetime analyses. The LITRF-PARAFAC quenching method could be used for in situ investigate the interactions between mixture of PAHs and HA, which could be applied to predict and evaluate the environmental behavior and ecological risk of PAHs in real time.
|
|
Received: 2015-09-27
Accepted: 2016-01-17
|
|
|
|
Corresponding Authors:
ZHANG Yong
E-mail: yzhang@xmu.edu.cn
|
|
[1] Sun H F, Yang Y N, Zhu Y X, et al. Talanta, 2013, 116(15): 441.
[2] Mei Y, Wu F C, Wang L Y, et al. Journal of Environmental Sciences, 2009, 21(4): 414.
[3] Hur J, Kim G. Chemosphere, 2009, 75(4): 483.
[4] Chen S, Ke R H, Zha J M, et al. Environmental Science & Technology, 2008, 42(24): 9431.
[5] Hur J, Lee B M, Shin H S. Chemosphere, 2011, 85(8): 1360.
[6] Gauthier T D, Shane E C, Guerin W F, et al. Environmental Science & Technology, 1986, 20(11): 1162.
[7] DU Ke-zhao, CHEN Jia-ning, CHEN Sheng-neng, et al(杜克钊, 陈佳宁, 陈省能, 等). Jouanal of Xiamen University·Natural Science(厦门大学学报·自然科学版), 2008, 1: 131.
[8] Lu R, Sheng G P, Liang Y, et al. Environmental Science and Pollution Research, 2013, 20(4): 2220.
[9] SUN Hai-feng, ZHU Ya-xian, WU Fang, et al(孙海峰, 朱亚先, 吴 芳, 等). Modern Instruments, 2012, 18(6): 1.
[10] Rudnick S M, Chen R F. Talanta, 1998, 47(4): 907.
[11] LIU Yang-zhi, YANG Cheng-hu, ZHU Ya-xian, et al(刘洋之, 杨承虎, 朱亚先, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2016, 36(10): 3332.
[12] Karlitschek P, Lewitzka F, Bünting U, et al. Applied Physics B, 1998, 67(4): 497.
[13] Andersson C A, Bro R. Chemometrics and Intelligent Laboratory Systems, 2000, 52(1): 1.
[14] Pan B, Ghosh S, Xing B S. Environmental Science & Technology, 2007, 41(18): 6472.
[15] Plaza C, Xing B S, Fernandez J M, et al. Environmental Pollution, 2009, 157(1): 257.
[16] Chen H, Kenny J E. Annals of Environmental Science, 2007, 1(1): 21.
[17] Laor Y, Rebhun M. Environmental Science & Technology, 2002, 36(5): 955.
[18] Pan B, Xing B S, Liu W X, et al. Chemosphere, 2007, 69(10): 1555.
[19] Yang K, Wang X L, Zhu L Z, et al. Environmental Science & Technology, 2006, 40(18): 5804.
[20] Perre C D, Menach K L, Ibalot F, et al. Analytica Chimica Acta, 2014, 807(7): 51.
[21] Wang H B, Zhang Y J. Journal of Environmental Science and Health, Part A, 2014, 49(1): 78.
[22] Pitois A, Abrahamsen L G, Ivanov P I, et al. Journal of Colloid and Interface Science, 2008, 325(1): 93.
[23] Chen S, Inskeep W P, Williams S A, et al. Environmental Science & Technology, 1994, 28(9): 1582.
[24] Selli E, Zaccaria C, Sena F, et al. Water Research, 2004, 38(9): 2268. |
| [1] |
WU Jun, YOU Jing-lin*, WANG Yuan-yuan, WANG Jian, WANG Min, Lü Xiu-mei. Raman Spectroscopic Study of Li2B4O7 Crystal and Melt Structure[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1736-1740. |
| [2] |
WU Xin1，2, LI Guang-lin1*, WEN Zhi-yu3. Study and Determination the Concentration of CNO-Ion of the QPQ with the Sequential Injection Spectrophotometric Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1824-1828. |
| [3] |
WANG Xiao-ping1,2, ZHANG Fei1,2,3*, YANG Sheng-tian4，AYINUER·Yushanjiang1,2，CHEN Yun5. Rapid Diagnosis of Surface Water Salt Content (WSC) in Ebinur Lake Watershed Based on 3-D Fluorescence Technology[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(05): 1468-1475. |
| [4] |
LIU Jun-shao1, HUANG Lei2, XIE Wen-ju1, LIN Hao1, CHEN Yi-ping2, PAN Hai-bo2*. Preparation of Four Phenoxy Phthalocyanine Zinc/ZnO Composites with in-situ Method by DBU Liquid Phase Catalyst and Its Photocatalytic Selectivity[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(05): 1486-1491. |
| [5] |
HAO Hua-hua1,2, YUAN Hui1,2, XU Guang-tong1,2*, XIANG Yan-juan1,2, MA Ai-zeng1,2. In Situ FTIR Study of CO Adsorption on Pt-Sn/Al2O3 Reforming Catalyst[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(03): 761-764. |
| [6] |
LI Xiang1, TAN Li-li1, LIU Ji-cheng1, LI Li1*, JIA Shou-shi2. Rapid Detection of Four Chemical Components Added Illegally in Slimming Health Food with TLC Situ Raman Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(03): 830-836. |
| [7] |
YANG Hong-jun, CHAI Xiao-li, WANG Min, LI Bing*. Study on Phase Transition Process of NaCl-H2O and NaCl-KCl-H2O at Low Temperature with in Situ XRD[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(03): 953-957. |
| [8] |
ZHOU Yan-lei1, ZHOU Fei-fei1, JIANG Cong-cong1, SHI Xiao-yong1，2*, SU Rong-guo1. Research of Identification Method for the Oil Spills Species Based on Fluorescence Excitation-Emission Matrix and Parallel Factor Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(02): 475-480. |
| [9] |
Lü Yu-guang, SHI Qi, GUO Qiang, SONG Shan-shan, QIN Yu-shan, WANG Bo, YANG Li-bin*. Studies on Luminescent Properties of TCNE-Phenanthroline Complex[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(02): 634-637. |
| [10] |
QIU Xin1, LI Xin-xia2, Arkin IBURAIM2, Gulina KUERBAN3, ZHANG Li-hua1, LI Yan1*. Determination of Cephradine Capsules Content Based on Fluorescence Quenching Method of FOS-μSIA-LOV[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(11): 3475-3478. |
| [11] |
ZHANG Fang-kun, LIU Tao*, GUAN Run-duo. In-situ ATR-FTIR Measurement of Solution Concentration Based on Temperature-Related Spectra Difference Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(10): 3011-3015. |
| [12] |
GUO Jin-jia, ZHANG Feng, LIU Chun-hao, LI Ying, ZHENG Rong-er. Raman-Fluorescence Spectroscopy for Underwater in-situ Application[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(10): 3099-3102. |
| [13] |
DU Ling-ling，CHEN Wei-gen，GU Zhao-liang，ZOU Jing-xin，WAN Fu. Application of Raman Spectroscopy to Analyze Acetic Acid Content Dissolved in Transformer Oil[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(09): 2774-2779. |
| [14] |
ZHANG Sa1, 2, 3, LIANG Li-ping1. In-Situ Raman Observations for PLZT Ferroelectric Ceramics under Compressive Stresses[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(07): 2073-2078. |
| [15] |
LI Shuai-dong1, 2, 3, JIANG Quan-liang3, LI Ye3, WU Ya-lin3, JIANG Jun-wu3, HUANG Tao1, 2, 3, YANG Hao3, HUANG Chang-chun1, 2, 3*. Spectroscopic Characteristics and Sources of Dissolved Organic Matter from Soils around Dianchi Lake, Kunming[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(05): 1448-1454. |
|
|
|
|
