光谱学与光谱分析 |
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Studies on Interaction of Sinafloxacin with Bovine Serum Albumin and Effect of the Coexistent Metal Ions on the Reaction |
FEI Yan1,LU Guo-cai2,FAN Guo-rong1*,QI Yun-peng1,WU Hui-ling1,WU Yu-tian1 |
1. School of Pharmacy,Second Military Medical University, Shanghai Key Laboratory for Pharmaceutical Metabolites Research,Shanghai 200433,China 2. Center for New Drug Evaluation,Institute of Basic Medical Science,Second Military Medical University,Shanghai 200433,China |
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Abstract Serum albumin is the most abundant protein in plasma. It can bind with many intrinsic and extrinsic materials. The study of the interaction between serum albumin and drugs is a very important task in life science and chemistry. Quinolone drug is a kind of antibacterial drugs which have been used widely in clinical medicine, but its pharmacology and toxicology still have to be studied further. Sinafloxacin is a new quinolone antibiotics. The interaction between sinafloxacin and bovine serum albumin (BSA) at different temperatures and pH was investigated by fluorescence and UV absorption spectroscopy. The experimental results demonstrate that the fluorescence quenching of BSA by sinafloxacin is a result of the formation of sinafloxacin-BSA complex and the quenching mechanism is mainly static quenching. The interaction association constants of BSA and sinafloxacin were determined from the double reciprocal Lineweaver-Burk plot. The binding distance(r=3.64 nm)and energy transfer efficiency (E=0.163) between donor(BSA)and acceptor (sinafloxacin) were obtained based on Fōrster’s non-radiative energy transfer theory. There is a strong interaction between sinafloxacin and BSA. From thermodynamic coordination it can be judged that the binding force between sinafloxacin and BSA is mainly electro-static force. The effect of sinafloxacin on the conformation of BSA was analyzed by synchronous fluorescence spectrometry and three-dimensional fluorescence spectrometry. The emission maximum of tyrosine residues does not show a significant shift, while the small blue shift of tryptophan residues indicates that the hydrophobicity of microenvironment was increased. In addition, in the plasma,there are some metal ions, which can participate in many important vital actions and affect the reactions of the drugs with the serum albumins. So the effect of Cu(Ⅱ), Fe(Ⅲ), Zn(Ⅱ) and Mg(Ⅱ) on the binding constant of sinafloxacin to BSA was also discussed and it was shown that the interaction between BSA and sinafloxacin was decreased. In short, the results offer a reference for the studies on the biological effects and action mechanism of sinafloxacin with albumins in vivo.
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Received: 2007-10-08
Accepted: 2008-01-08
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Corresponding Authors:
FAN Guo-rong
E-mail: Guorfan@yahoo.com.cn
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[1] ZHANG Hai-rong,GUO Si-yuan,LI Lin,et al(张海容,郭祀远,李 琳,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2001,21(6):829. [2] LI Zhi-yuan,LU Guo-cai,YUAN Bo-jun,et al(栗志远,陆国才,袁伯俊,等). Chinese Journal of New Medicine(中国新药杂志),2006,15(4):274. [3] SHENG Liang-quan,YAN Xiang-yang,XU Hua-jie,et al(盛良全,闫向阳,徐华杰,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2007,27(2):306. [4] SHANG Zhi-cai,FAN Cheng-ping,GUO Ming,et al(商志才,范成平,郭 明,等). Acta Phys. -Chim. Sinica(物理化学学报),2004,20(8):864. [5] YAN Cheng-nong,ZHANG Hua-xin,LIU Yi,et al(颜承农,张华新,刘 义,等). Acta Chim. Sin.(化学学报),2005,63(18):1727. [6] Wang Yan-Qing,Zhang Hong-Mei,Zhang Gen-Cheng. J. Pharm. Biomed. Anal.,2006,41(3):1041. [7] YAN Zheng-yu,SHAO Xiu-fen,JIANG Xin-min,et al(严拯宇,邵秀芬,姜新民,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2006,26(8):1494. [8] Lakowicz J R,Weber G. Biochemistry,1973,12(21):4161. [9] MA Gui-bin,YANG Pin(马贵斌,杨 频). Chinese Biochemical Journal(生物化学杂志),1992,8(5):624. [10] Ross P D,Subramanian S. Biochemistry,1981,20(11):3096. [11] YANG Pin,GAO Fei(杨 频,高 飞). Principle of Bioinorganic Chemistry(生物无机化学原理). Beijing:Science Press(北京:科学出版社),2002. 322. [12] Cheng Fang-Qing,Wang Ya-Ping,Li Zhong-Ping,et al. Spectrochim. Acta A,2006,65(5):1144. [13] BIAN He-dong,LI Mei,YU Qing,et al(边贺东,李 梅,于 青,等). Chinese Journal of Inorganic Chemistry(无机化学学报),2006,22(5):845. [14] CHEN Guo-zhen,HUANG Xian-zhi,ZHENG Zhu-zi,et al(陈国珍,黄贤智,郑朱梓,等). Fluorescence Analytical Method(荧光分析方法). Beijing:Science Press(北京:科学出版社),1990. 122. [15] HE Hua,YE Hai-ying,DAI Li,et al(何 华,叶海英,戴 丽,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2006,26(3):480. [16] Shaikh S M T, Seetharamappa J, Ashoka S, et al. Dyes and Pigments, 2007,73(2):211. [17] Pang Yue-Hong,Yang Li-Li,Shuang Shao-Min,et al. J. Photochem. Photobiol. B,2005,80(2):139. |
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