光谱学与光谱分析 |
|
|
|
|
|
Effect of Spectra Correction on the Fluorescence Characteristics of Dissolved Organic Matter |
WU Hua-yong1, ZHOU Ze-yu1, WANG Hong-tao1*, LU Wen-jing1, SUN Xiao-jie2 |
1. School of Environment, Tsinghua University, Beijing 100084, China 2. School of Environmental Science and Engineering, Guilin University of Technology, Guilin 541004, China |
|
|
Abstract The Excitation-emission matrix (EEM) fluorescence spectra of dissolved organic matter (DOM) are not only dependent on the chemical structure of DOM as well as the local chemical environment around the DOM, but also dependent on the instrument employed for the analysis. Thus, in order to get the real spectra of the DOM, spectra correction of the effect of the instrument-specific response is necessary. However, some studies corrected DOM spectra, while still some studies didn’t, leading to inconformity when comparing the data and the results from different groups. The present work evaluated the effect of spectra correction on the fluorescence characteristics of DOM. The results demonstrated that DOM spectra differed significantly after correction. Fluorescence intensities showed a decrease in the range of Ex/Em=220~450/250~500 nm, while an increase at Em<250 nm after correction. Fluorescence intensity ratio (FI) and humification index (HIX) derived from peak picking method showed a decrease after correction, while biological index (BIX) increased slightly. PⅠ,n, PⅡ,n and PⅢ,n derived from FRI analysis exhibited a significant increase, while PⅣ,n and PⅤ,n decreased. The results suggest that spectral correction should be included when analyzing the properties and trends of the DOM using EEM.
|
Received: 2012-04-24
Accepted: 2012-07-04
|
|
Corresponding Authors:
WANG Hong-tao
E-mail: htwang@tsinghua.edu.cn
|
|
[1] Coble P G. Marine Chemistry, 1996, 51: 325. [2] Stedmon C A, Amon R M W, Rinehart A J, et al. Marine Chemistry, 2011, 124(1-4): 108. [3] Yao X, Zhang Y L, Zhu G W, et al. Chemosphere, 2011, 82(2): 145. [4] Bridgeman J, Bieroza M, Baker A. Reviews in Environmental Science and Biotechnology, 2011, 10: 277. [5] Henderson R K, Baker A, Murphy K R, et al. Water Research, 2009, 43: 863. [6] DENG Xun, GUO Wei-dong, ZHUO Jian-fu(邓 荀, 郭卫东, 卓健富). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2012, 32(1): 137. [7] Lakowicz J R. Principles of Fluorescence Spectroscopy, 3rd ed., Springer, 2008. [8] Hudson N , Baker A, Reynolds D. River Resources, 2007, 23(4): 631. [9] Murphy K R, Burler K D, Spencer R M, et al. Environmental Science & Technology, 2010, 44: 9405. [10] Bahram M, Bro R, Stedmon C, et al. Journal of Chemometrics, 2006, 20(3-4): 99. [11] Kalbitz K, Schmerwitz J, Schwesig D, et al. Geoderma, 2003, 103: 273. [12] Birdwell J E, Engel A S. Organic Geochemistry, 2010, 41: 270. [13] McKnight D M, Boyer E W, Westerhoff P K, et al. Limnology and Oceanography, 2001, 46: 38. [14] Huguet A, Vacher L, Relexans S, et al. Organic Geochemistry, 2009, 40: 706. [15] Zsolnay A, Baigar E, Jimenez M, et al. Chemosphere, 1999, 38: 45. [16] Wu H Y, Zhou Z Y, Zhang Y X, et al. Bioresource Technology, 2012, 110: 174. [17] He X S, Xi B D, Wei Z M, et al. Journal of Hazardous Materials, 2011, 190: 293. [18] Hernandez-Ruiz S, Abrell L, Wickramasekara S, et al. Water Research, 2012, 46(4): 943. [19] Chen W, Westerhoff P, Leenheer J A, et al. Environmental Science & Technology, 2003, 37: 5701. |
[1] |
LEI Hong-jun1, YANG Guang1, PAN Hong-wei1*, WANG Yi-fei1, YI Jun2, WANG Ke-ke2, WANG Guo-hao2, TONG Wen-bin1, SHI Li-li1. Influence of Hydrochemical Ions on Three-Dimensional Fluorescence
Spectrum of Dissolved Organic Matter in the Water Environment
and the Proposed Classification Pretreatment Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 134-140. |
[2] |
GU Yi-lu1, 2,PEI Jing-cheng1, 2*,ZHANG Yu-hui1, 2,YIN Xi-yan1, 2,YU Min-da1, 2, LAI Xiao-jing1, 2. Gemological and Spectral Characterization of Yellowish Green Apatite From Mexico[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 181-187. |
[3] |
SONG Yi-ming1, 2, SHEN Jian1, 2, LIU Chuan-yang1, 2, XIONG Qiu-ran1, 2, CHENG Cheng1, 2, CHAI Yi-di2, WANG Shi-feng2,WU Jing1, 2*. Fluorescence Quantum Yield and Fluorescence Lifetime of Indole, 3-Methylindole and L-Tryptophan[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3758-3762. |
[4] |
YANG Ke-li1, 2, PENG Jiao-yu1, 2, DONG Ya-ping1, 2*, LIU Xin1, 2, LI Wu1, 3, LIU Hai-ning1, 3. Spectroscopic Characterization of Dissolved Organic Matter Isolated From Solar Pond[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3775-3780. |
[5] |
XUE Fang-jia, YU Jie*, YIN Hang, XIA Qi-yu, SHI Jie-gen, HOU Di-bo, HUANG Ping-jie, ZHANG Guang-xin. A Time Series Double Threshold Method for Pollution Events Detection in Drinking Water Using Three-Dimensional Fluorescence Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3081-3088. |
[6] |
JIA Yu-ge1, YANG Ming-xing1, 2*, YOU Bo-ya1, YU Ke-ye1. Gemological and Spectroscopic Identification Characteristics of Frozen Jelly-Filled Turquoise and Its Raw Material[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2974-2982. |
[7] |
YANG Xin1, 2, XIA Min1, 2, YE Yin1, 2*, WANG Jing1, 2. Spatiotemporal Distribution Characteristics of Dissolved Organic Matter Spectrum in the Agricultural Watershed of Dianbu River[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2983-2988. |
[8] |
ZHU Yan-ping1, CUI Chuan-jin1*, CHENG Peng-fei1, 2, PAN Jin-yan1, SU Hao1, 2, ZHANG Yi1. Measurement of Oil Pollutants by Three-Dimensional Fluorescence
Spectroscopy Combined With BP Neural Network and SWATLD[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2467-2475. |
[9] |
QIU Cun-pu1, 2, TANG Xiao-xue2, WEN Xi-xian4, MA Xin-ling2, 3, XIA Ming-ming2, 3, LI Zhong-pei2, 3, WU Meng2, 3, LI Gui-long2, 3, LIU Kai2, 3, LIU Kai-li4, LIU Ming2, 3*. Effects of Calcium Salts on the Decomposition Process of Straw and the Characteristics of Three-Dimensional Excitation-Emission Matrices of the Dissolved Organic Matter in Decomposition Products[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(07): 2301-2307. |
[10] |
SHI Chuan-qi1, LI Yan2, HU Yu3, YU Shao-peng1*, JIN Liang2, CHEN Mei-ru1. Fluorescence Spectral Characteristics of Soil Dissolved Organic Matter in the River Wetland of Northern Cold Region, China[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(05): 1517-1523. |
[11] |
JIANG Xin-tong1, 2, 3, XIAO Qi-tao3, LI Yi-min1, 2, LIAO Yuan-shan1, 2, LIU Dong3*, DUAN Hong-tao1, 2, 3*. Temporal and Spatial Effects of River Input on Dissolved Organic Matter Composition in Lake Bosten[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(05): 1636-1644. |
[12] |
CHAI Shu1, PENG Hai-meng1, WU Wen-dong1, 2*. Acoustic-Based Spectral Correction Method for Laser-Induced Breakdown Spectroscopy in High Temperature Environment[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(05): 1401-1407. |
[13] |
LI Yuan-jing1, 2, CHEN Cai-yun-fei1, 2, LI Li-ping1, 2*. Spectroscopy Study of γ-Ray Irradiated Gray Akoya Pearls[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(04): 1056-1062. |
[14] |
LIU Xia-yan1, CAO Hao-xuan1, MIAO Chuang-he1, LI Li-jun2, ZHOU Hu1, LÜ Yi-zhong1*. Three-Dimensional Fluorescence Spectra of Dissolved Organic Matter in Fluvo-Aquic Soil Profile Under Long-Term Composting Treatment[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(03): 674-684. |
[15] |
LÜ Yang1, PEI Jing-cheng1*, ZHANG Yu-yang2. Chemical Composition and Spectra Characteristics of Hydrothermal Synthetic Sapphire[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(11): 3546-3551. |
|
|
|
|