| 光谱学与光谱分析 |
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| Study on of Deferrization Mechanism of Apoferritin and the Effect of Spectra Variation with Holoferritin |
| LIU Wen-ying1,2，ZHANG Jian1, 3*，TONG Jun-mao1，ZHAO Guang-hua3* |
1. College of Food, Shihezi University, Shihezi 832003, China
2. Chinese National Egg Engineering Research Center, Beijing 102115，China
3. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China |
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Abstract The deferrization mechanism of apoferritin was established, and the spectra variation of apoferritin was compared with that of holoferritin. Sodium hyposulfite is a strong reducing agent, therefore, was applied to deoxidize holoferritin to release iron ion，and connection of iron of buffer was measured by the 2,2-dipyridyl. Apoferritin was detected by ICP-MS. Holoferritin was found to have no absorption compared with apoferritin by UV analysis, and have no fluorescence emission spectra in contrast with apoferritin by fluorescence analysis.
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Received: 2010-11-03
Accepted: 2011-03-15
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Corresponding Authors:
ZHANG Jian,ZHAO Guang-hua
E-mail: gzhao1000@yahoo.com
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