| 光谱学与光谱分析 |
|
|
|
|
|
| Speciation Analysis of Trace Elements Cu,Fe and Zn in Serum by Flame Atomic Absorption Spectrophotometry |
| HU Jun1,CHANG Yao-ming1*,GAO Shuang-bin2,HAI Chun-xu3,LI Jin-sheng1,XIE Xiao-ping1 |
1. Department of Aerospace Hygiene,School of Aerospace Medicine,Fourth Military Medical University of Chinese PLA,Xi’an 710032,China
2. Department of Nutriology,School of Preclinical Medicine,Fourth Military Medical University of Chinese PLA,Xi’an 710032,China
3. Department of Toxicology,School of Preclinical Medicine,Fourth Military Medical University of Chinese PLA,Xi’an 710032,China |
|
|
|
|
Abstract Since biological functions of the elements are generally different,depending on their chemical forms,chemical speciation analysis is really important in metallomics research. Thus,multielement analysis and chemical speciation of the elements in serum were carried out in the present work. A hyphenated technique was developed for high-throughput speciation analysis of the copper,iron and zinc in serum by molecular biology technology and flame atomic absorption spectrophotometry (AAS). Here,Cu,Fe and Zn in serum were classifyied as the forms of combination and non-combination. The serum protein was precipitated by 60% concentration of ethanol under hypothermy. The forms of combination of Cu,Fe and Zn in serum which combined with proteins were in precipitations,and the forms of non-combination of Cu,Fe and Zn in serum,which were free ions,were in supernatant. The total amount of Cu,Fe and Zn in serum and the amount of the forms of non-combination of Cu,Fe and Zn were analyzed by AAS. The amount of the forms of combination of Cu,Fe and Zn was obtained by calculation. The detection limit of Cu in serum by the method is around and 9.84×10-3 μg·mL-1. For Fe and Zn,the detection limit is about 2.76×10-2 μg·mL-1 and 1.06×10-3 μg·mL-1,respectively. The percentage recovery of trace elements Cu,Fe and Zn by the proposed procedure is in the range 95.0%-101.0%，95.0％-102.0％ and 95.0％-103.0％,respectively. The relative standard deviation(RSD) of trace elements Cu,Fe and Zn in the serum is in the range 1.88％-2.26％，0.56％-1.59％ and 0.34％-1.36％,respectively. Speciation of trace elements Cu,Fe and Zn in the serum of SD rat were analyzed by the method.
|
|
Received: 2007-05-10
Accepted: 2007-08-20
|
|
|
|
Corresponding Authors:
CHANG Yao-ming
E-mail: kyafwqs@fmmu.edu.cn
|
|
[1] CONG Tao,ZHAO Lin(丛 涛，赵 霖). Studies of Trace Elements and Health(微量元素与健康研究),2006,23(6): 59.
[2] KAI Xiao-ming(开小明). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2004,24(11): 1412.
[3] CHEN Jing-yuan(陈景元). Trace Elements and Health(微量元素与健康). Xi’an: Fourth Military Medical University Press(西安: 第四军医大学出版社),2003. 20.
[4] ZHANG Wei,ZHANG Zhuo-yong,SHI Yan-zhi,et al(张 薇，张卓勇，施燕支,等). Spectroscopy and Spectral Analysis(光谱学与光谱分析),2006,26(5): 963.
[5] WANG Jia-zheng,FAN Ming(汪家政，范 明). Protein Technical Manual(蛋白质技术手册). Beijing: Science Press(北京：科学出版社)，2000. 68.
[6] Szpunar J. Analyst,2005,130(4): 442. |
| [1] |
HE Yan1, TAO Ran1, YANG Ming-xing1, 2*. The Spectral and Technology Studies of Faience Beads Unearthed in Hubei Province During Warring States Period[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3700-3709. |
| [2] |
WANG Wei-en. Analysis of Trace Elements in Ophiocordyceps Sinensis From
Different Habitats[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3247-3251. |
| [3] |
WANG Yan1, HUANG Yi1, 2*, YANG Fan1, 2*, WU Zhong-wei2, 3, GUAN Yao4, XUE Fei1. The Origin and Geochemical Characteristics of the Hydrothermal Sediments From the 49.2°E—50.5°E Hydrothermal Fields of the Southwest Indian Ocean Ultra-Slow Spreading Ridge[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2868-2875. |
| [4] |
CHEN Chao-yang1, 2, LIU Cui-hong1, 2, LI Zhi-bin3, Andy Hsitien Shen1, 2*. Alexandrite Effect Origin of Gem Grade Diaspore[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2557-2562. |
| [5] |
CHEN Di, SONG Chen, SONG Shan-shan, ZHANG Zhi-jie*, ZHANG Hai-yan. The Dating of 9 Batches of Authentic Os Draconis and the Correlation
Between the Age Range and the Ingredients[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(06): 1900-1904. |
| [6] |
HE Yan1, SU Yue1, YANG Ming-xing1, 2*. Study on Spectroscopy and Locality Characteristics of the Nephrites in Yutian, Xinjiang[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2022, 42(12): 3851-3857. |
| [7] |
CHEN Chao-yang1，HUANG Wei-zhi1，SHAO Tian1，LI Zhi-bin2，Andy Hsitien Shen1*. Characteristics of Visible Spectrum of Apatite With Alexandrite Effect[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(05): 1483-1486. |
| [8] |
LIU Yan-de, GAO Xue, JIANG Xiao-gang, GAO Hai-gen, LIN Xiao-dong, ZHANG Yu, ZHENG Yi-lei. Detection of Anthracnose in Camellia Oleifera Based on Laser-Induced Breakdown Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(09): 2815-2820. |
| [9] |
LU Xiao-ke1, LI Wei-dong1, LI Xin-wei2. Spectroscopic Analysis of Relics Unearthed from Xipo Site[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(04): 1186-1194. |
| [10] |
LIANG Piao-piao1, ZHOU Shan-shan1, XING Yun-xin1, LIU Ying1, 2*. Quantification of Trace Elements in Hair Samples from 156 Women Living in the Low-Selenium Region of Inner Mongolia by ICP-AES and AFS[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(07): 2217-2222. |
| [11] |
HAO Xiao-jian*, TANG Hui-juan, HU Xiao-tao. Detection Sensitivity Improvement Study of LIBS by Combining Au-Nanoparticles and Magnetic Field[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(05): 1599-1603. |
| [12] |
LIU Hong-wei, NIE Xi-du*. Analysis of Trace Elements in Wild Artemisia Selengensis Using Inductively Coupled Plasma Tandem Mass Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(12): 3923-3928. |
| [13] |
CAI Shi-shi1，ZHANG En1, 2*. Trace Elements and U-Pb Ages of Zircons from Myanmar Jadeite-Jade by LA-ICP-MS: Constraints for Its Genesis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(06): 1896-1903. |
| [14] |
JIANG Bo1, 3, HUANG Jian-hua2*, LIU Wei2. Multi-Element Analysis of Wild Chinese Honeylocust Fruit by Inductively Coupled Plasma Tandem Mass Spectrometry (ICP-MS/MS)[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(12): 3859-3864. |
| [15] |
PENG Chuan-yi, ZHU Xiao-hui, XI Jun-jun, HOU Ru-yan, CAI Hui-mei*. Macro- and Micro-Elements in Tea (Camellia sinensis) Leaves from Anhui Province in China with ICP-MS Technique: Levels and Bioconcentration[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(06): 1980-1986. |
|
|
|
|
