光谱学与光谱分析 |
|
|
|
|
|
Ion Fluorescence Probe Detecting the Formation of Triplex DNA |
ZHOU Wen-jun, SHEN He-bai*, YANG Yong-tao, YUAN Lian-qun |
Shanghai Normal University,The College of Life and Environment Science,Shanghai 200234,China |
|
|
Abstract The fluorescence of Tb3+ is not quenched after the rare earth ion is combined with DNA. The fluorescence intensity is related not only to the kinds of bases of DNA but also to the kinds of DNA. The rare earth ion Tb3+ was used as the fluorescence probe to detect the formation of triplex DNA. The results show that the fluorescence intensity of Tb3+ combined with polydA is much stronger than that of Tb3+ combined with polydT,which testifies that the fluorescence intensity is related to the kinds of bases combined with Tb3+. The results also demonstrate that the rare earth ion can be used to detect those three forms of DNA though their fluorescence peak positions are similar when Tb3+ as a fluorescence probe is combined with single strand DNA (ssDNA), double helix DNA (dsDNA),and triple helix DNA (tsDNA),respectively. However,their intensities are quite different. The fluorescence intensity of Tb3+ combined with ssDNA-Tb3+ is the largest. And the fluorescence intensity of Tb3+ combined with tsDNA-Tb3+ takes the second place. The minimum peak intensity belongs to the contribution of Tb3+ coupled with dsDNA-Tb3+. The reason is that the capability of energy transference of ssDNA, dsDNA and tsDNA is different. The different degree of energy transference influences the intensities of Tb3+. The influences of pH and the metal ion on the formation of triplex DNA were also studied. The authors found that the neutral pH and high valence metal ion are beneficial to the formation of ts-DNA.
|
Received: 2002-12-30
Accepted: 2003-02-10
|
|
Corresponding Authors:
SHEN He-bai
|
|
Cite this article: |
ZHOU Wen-jun,SHEN He-bai,YANG Yong-tao, et al. Ion Fluorescence Probe Detecting the Formation of Triplex DNA [J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2004, 24(12): 1605-1608.
|
|
|
|
URL: |
https://www.gpxygpfx.com/EN/Y2004/V24/I12/1605 |
[1] Felsonfeld G, Davies D R, Rich A C. J. Am. Chem. Soc., 1957, 79: 2023. [2] LI Xiao-xu, ZHANG Liang-ren, ZHANG Li-he, CHENG Yao-zu(李晓旭, 张亮仁, 张礼和, 程耀祖). Progress in Biochemistry and Biophysics(生物化学与生物物理进展), 1998, 25(1): 21. [3] GUO Jun, ZHANG Ping-chen, BAI Chun-li et al(郭 军,张平城,白春礼等). Progress in Biochemistry and Biophysics(生物化学与生物物理进展), 1993, 20(2): 89. [4] JIANG Luo-hua, WANG Xin-juan et al(蒋罗化,王新娟等). Foreign Medical Sciences(Section of Genetics)(国外医学遗传学分册), 1998, 21(2): 69. [5] LIU Ding-fan, WANG Chang-cai et al(刘定樊,王昌才等). Foreign Medicine and Molecular Biology Fascicule(国外医学分子生物学分册),1996, 18(4): 173. [6] ZHANG Ping-chen, BAI Chun-li, CAO En-hua, FANG Ye, HE Yu-jian(张平诚,白春礼,曹恩华, 方 晔, 何裕建). Chinese Science Bulletin(科学通报),1993, 38 (13): 1194. [7] Topal M D,Fresco J R. Biochemistry, 1980,19: 5531. [8] YANG Pin, YANG Bin-shen et al(杨 频, 杨斌盛等). Introduction of Ion Probe Method(离子探针方法导论). Beijing: Science Press(北京: 科学出版社), 1994. 138. [9] XI Xiao-li, YANG Man-man, HAN Xiao-jian et al(席小莉,杨曼曼,韩小见等). Chinese Journal of Inorganic Chemistry(无机化学学报), 2001, 17(6): 781. [10] YANG Man-man, YANG Pin, XI Xiao-li et al(杨曼曼,杨 频,席小莉等). Chinese Science Bulletin(科学通报), 1997, 42:1276. [11] Arnott S, Selsing E. J. Mol. Biol., 1974, 88: 509.
|
[1] |
LEI Hong-jun1, YANG Guang1, PAN Hong-wei1*, WANG Yi-fei1, YI Jun2, WANG Ke-ke2, WANG Guo-hao2, TONG Wen-bin1, SHI Li-li1. Influence of Hydrochemical Ions on Three-Dimensional Fluorescence
Spectrum of Dissolved Organic Matter in the Water Environment
and the Proposed Classification Pretreatment Method[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 134-140. |
[2] |
GU Yi-lu1, 2,PEI Jing-cheng1, 2*,ZHANG Yu-hui1, 2,YIN Xi-yan1, 2,YU Min-da1, 2, LAI Xiao-jing1, 2. Gemological and Spectral Characterization of Yellowish Green Apatite From Mexico[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 181-187. |
[3] |
HAN Xue1, 2, LIU Hai1, 2, LIU Jia-wei3, WU Ming-kai1, 2*. Rapid Identification of Inorganic Elements in Understory Soils in
Different Regions of Guizhou Province by X-Ray
Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2024, 44(01): 225-229. |
[4] |
WANG Hong-jian1, YU Hai-ye1, GAO Shan-yun1, LI Jin-quan1, LIU Guo-hong1, YU Yue1, LI Xiao-kai1, ZHANG Lei1, ZHANG Xin1, LU Ri-feng2, SUI Yuan-yuan1*. A Model for Predicting Early Spot Disease of Maize Based on Fluorescence Spectral Analysis[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3710-3718. |
[5] |
CHENG Hui-zhu1, 2, YANG Wan-qi1, 2, LI Fu-sheng1, 2*, MA Qian1, 2, ZHAO Yan-chun1, 2. Genetic Algorithm Optimized BP Neural Network for Quantitative
Analysis of Soil Heavy Metals in XRF[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3742-3746. |
[6] |
SONG Yi-ming1, 2, SHEN Jian1, 2, LIU Chuan-yang1, 2, XIONG Qiu-ran1, 2, CHENG Cheng1, 2, CHAI Yi-di2, WANG Shi-feng2,WU Jing1, 2*. Fluorescence Quantum Yield and Fluorescence Lifetime of Indole, 3-Methylindole and L-Tryptophan[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3758-3762. |
[7] |
YANG Ke-li1, 2, PENG Jiao-yu1, 2, DONG Ya-ping1, 2*, LIU Xin1, 2, LI Wu1, 3, LIU Hai-ning1, 3. Spectroscopic Characterization of Dissolved Organic Matter Isolated From Solar Pond[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(12): 3775-3780. |
[8] |
LI Xiao-li1, WANG Yi-min2*, DENG Sai-wen2, WANG Yi-ya2, LI Song2, BAI Jin-feng1. Application of X-Ray Fluorescence Spectrometry in Geological and
Mineral Analysis for 60 Years[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 2989-2998. |
[9] |
XUE Fang-jia, YU Jie*, YIN Hang, XIA Qi-yu, SHI Jie-gen, HOU Di-bo, HUANG Ping-jie, ZHANG Guang-xin. A Time Series Double Threshold Method for Pollution Events Detection in Drinking Water Using Three-Dimensional Fluorescence Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(10): 3081-3088. |
[10] |
MA Qian1, 2, YANG Wan-qi1, 2, LI Fu-sheng1, 2*, CHENG Hui-zhu1, 2, ZHAO Yan-chun1, 2. Research on Classification of Heavy Metal Pb in Honeysuckle Based on XRF and Transfer Learning[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2729-2733. |
[11] |
YE Zi-yi, LIU Shuang, ZHANG Xin-feng*. Screening of DNA Dyes for Colorimetric Sensing Via Rapidly Inducing Gold Nanoparticles Aggregation[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2805-2810. |
[12] |
JIA Yu-ge1, YANG Ming-xing1, 2*, YOU Bo-ya1, YU Ke-ye1. Gemological and Spectroscopic Identification Characteristics of Frozen Jelly-Filled Turquoise and Its Raw Material[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2974-2982. |
[13] |
YANG Xin1, 2, XIA Min1, 2, YE Yin1, 2*, WANG Jing1, 2. Spatiotemporal Distribution Characteristics of Dissolved Organic Matter Spectrum in the Agricultural Watershed of Dianbu River[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(09): 2983-2988. |
[14] |
CHEN Wen-jing, XU Nuo, JIAO Zhao-hang, YOU Jia-hua, WANG He, QI Dong-li, FENG Yu*. Study on the Diagnosis of Breast Cancer by Fluorescence Spectrometry Based on Machine Learning[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2407-2412. |
[15] |
ZHU Yan-ping1, CUI Chuan-jin1*, CHENG Peng-fei1, 2, PAN Jin-yan1, SU Hao1, 2, ZHANG Yi1. Measurement of Oil Pollutants by Three-Dimensional Fluorescence
Spectroscopy Combined With BP Neural Network and SWATLD[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2023, 43(08): 2467-2475. |
|
|
|
|