| 光谱学与光谱分析 |
|
|
|
|
|
| Studied on the Determination of Protein by Probe of Zinc 5-Aminosalicylate |
| CUI Feng-ling,QIN Li-xia,HAO Er-jun,WANG Jun-li,WANG Li,ZHANG Qiang-zhai |
| School of Chemistry and Environmental Science of Henan Normal University，Xinxiang 453007, China |
|
|
|
|
Abstract Zinc 5- aminosalylicylate(5-ASZ), a new creative medicine, can react with serum albumin to form a complex. Based on this, a new method for the determination of proteins by synchronous fluorescence spectra under simulated physiological conditions was established using 5 - ASZ as a fluorescence probe. The spectral characterization and intensity of synchronous fluorescence were related to the value of Δλ, reaction medium and reaction temperature, and so on. On basis of these, eperimental results show that, under the optimum conditions, the synchronous fluorescence intensity of the system is in proportion to the concentration of protein in the range of 1.66-496.8 μg·mL-1 for human serum albumin (HSA). The detection limit was 0.88 μg·mL-1 (n=11) for HSA. On basis of these it had been applied to the determination of proteins in human serum, urine and spit samples with satisfactory results，the recovery was within the range of 98.0%-103.8%. Results suggested that the method possessed easy of implementation, rapidity, high sensitivity, broad linear range, and better RSD and recovery, and so on, and the method was employed directly to determine the total proteins in samples, which results were satisfactory.
|
|
Received: 2007-03-02
Accepted: 2007-06-06
|
|
|
|
Corresponding Authors:
CUI Feng-ling
E-mail: fenglingcui@hotmail.com
|
|
［1］ Cui F L, Cui Y R, Luo H X, et al. Chinese Science Bulletin, 2006, 51(18)：2201.
［2］ ZHENG Shi-ying, ZHOU Chun, LUO Deng-bai(郑石荧，周 春，罗登柏). Journal of Instrumental Analysis(分析测试学报)，2003,22(4)：42.
［3］ HU Ying-ying, HU Wang-ping, ZHOU Chang-shao, et al(胡盈莹，胡望平，周长邵，等). Journal of Medical Research(医学研究杂志)，2007，36(3)：56.
［4］ FENG Ning-chuan, HE Su-ping, ZHANG Juan, et al(冯宁川, 何苏萍，张 娟, 等). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2004, 24(2)：194.
［5］ Guo Z, Hao Y, Cong X, et al. Anal. Chim. Acta, 2000, 403：225.
［6］ ZHU Ying-gui, GAO Feng, ZHANG Li, et al(朱英贵，高 峰，章 丽，等). Chinese J. of Anal. Chem.(分析化学)，2005，33(5)：743.
［7］ Perez-Ruiz Tomas. Analyst, 2000, 125：507.
［8］ ZHANG Yuan, LIN Zhe-xuan, LI Hui, et al(张 源，林哲绚，李 慧，等). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2006，26(11)：2089.
［9］ FENG Su-ling, WANG Jin, FAN Jing(冯素玲,王 瑾，樊 静). Spectroscopy and Spectral Analysis(光谱学与光谱分析), 2005, 25(6)：927.
［10］ ZHANG Guo-wen, NI Yong-nian(张国文，倪永年). Spectroscopy and Spectral Analysis(光谱学与光谱分析)，2006，26(1)：113.
［11］ Cui F L, Wang J L, Cui Y R, et al. Anal. Chim. Acta, 2006, 571(2)：175.
［12］ Cui F L, Fan J, Li W, et al. Journal of Pharmaceutical and Biomedical Analysis, 2004, 34(1)：189. |
| [1] |
WANG Guo-shui1, GUO Ao2, LIU Xiao-nan1, FENG Lei1, CHANG Peng-hao1, ZHANG Li-ming1, LIU Long1, YANG Xiao-tao1*. Simulation and Influencing Factors Analysis of Gas Detection System Based on TDLAS Technology[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(10): 3262-3268. |
| [2] |
CHEN Jian1, HUANG Jun-shi1, 2, LIU Mu-hua1, 2, YUAN Hai-chao1, 2, HUANG Shuang-gen1, 2, ZHAO Jin-hui1, 2*, XU Ning1, WANG Ting1, HU Wei1. Study on Rapid Detection Method of Danofloxacin Mesylate and Ofloxacin Residues in a Chicken Based on Synchronous Fluorescence Spectrum[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2021, 41(05): 1367-1372. |
| [3] |
YANG Hui-qin1, 2, ZHANG Bo1, 2, MA Ling1, 2, SHANG Yi1, 2, GAO Dong-li1, 2*. Extraction and Spectroscopic Analysis of Chlorogenic Acid in Diploid Potato[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(12): 3860-3864. |
| [4] |
LI Ling1, 2, 3, NAI Xue-ying1, 2*, CHAI Xiao-li1, 2, 3, LIU Xin1, 2, GAO Dan-dan1, 2, DONG Ya-ping1, 2. Optimization of Determination Method of Lithium in Oil-Field Water Based on DOE[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(08): 2617-2621. |
| [5] |
CHEN Ying-ying1, 2, ZHENG Zhao-pei1*, YANG Fang2, BAI Yang2, YU Hui-bin2. The Composition and Structure of Dissolved Organic Matter in Saline Soil Were Studied by Synchronous Fluorescence Spectroscopy Combined with Principal Components and Two-Dimensional Correlation[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2020, 40(02): 489-493. |
| [6] |
ZHANG Jiu-ming1, 2, ZHOU Bao-ku1, WEI Dan3*, CHI Feng-qin1*, HAO Xiao-yu1, JIN Liang1, KUANG En-jun1. Spectroscopic Characteristics of HA Structure in Black Soil with Organic and Inorganic Fertilizer[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(03): 845-850. |
| [7] |
NIE Li-xing, CHANG Yan, DAI Zhong, MA Shuang-cheng*. A New Method for Determination of Polysorbate 80 in Shengmai Injection Based on Absorption Coefficient[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2019, 39(01): 199-203. |
| [8] |
YU Jie1, ZHU Shu-wen1, LU Quan-fang1, 2*, ZHANG Zhi-chao1, ZHANG Xiao-min1, WANG Xing1, YANG Wu1. High Sensitive Determination of Pb and Zn in Refined Copper Ores Samples Using Liquid Cathode Glow Discharge-Atomic Emission Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(11): 3550-3557. |
| [9] |
XIE Feng-ying1, LI Feng-feng1, ZHANG Shuang1, BI Wei-wei2, ZHANG Xiu-ling1*, ZHANG Xiao-nan1. Analysis of Acylation Modification of Black Rice Anthocyanins Using Fourier Transform Infrared Spectroscopy (FTIR)[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(08): 2386-2389. |
| [10] |
LI Shuang-fang1,2, GUO Yu-bao1*, SUN Yan-hui2, GU Hai-yang2. Rapid Identification of Sunflower Seed Oil Quality by Three-Dimensional Synchronous Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2018, 38(04): 1165-1170. |
| [11] |
ZHANG Yuan1, LIN Zhe-xuan1, HAN Ming2*. Study on the Change of the Content of Aromatic Amino Acid Residues in the Process of Malignant Transformation by Synchronous Fluorescence Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(09): 2822-2825. |
| [12] |
ZHONG Yong-cui1, 3, 4, YANG Li-wei2, QIU Yun-qi2, WANG Shu-mei1,3,4, LIANG Sheng-wang1, 3, 4*. Rapid Determination of Geniposide in Gardenia Jasminoids Ellis in Different Preparations Methods with NIRS[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(06): 1771-1777. |
| [13] |
LI Jian-ting1, 2, ZHANG Yi-ming2, DU Mei2, HAO Qian2, LI Jian-qiang1*. Study on the Interferences and Direct Determination of Sc in Metallurgical Tails with Inductively Coupled Plasma Mass Spectrometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(04): 1259-1263. |
| [14] |
QUAN Chao-ming1, 2, 3, HU Yao-qiang1, 2, 3, LIU Hai-ning1, 2, LI Ming-zhen1, 2, YE Xiu-shen1, 2*, WU Zhi-jian1, 2* . Rapid Determination of Dodecylmorpholine in Aqueous Solutions with UV-Vis Spectrophotometry[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2017, 37(02): 509-512. |
| [15] |
GUO Meng-lin, YANG Hui-qing, HUANG Ping, CHEN Lin, LI Dong-hui* . Application of Cationic Aluminum Phthalocyanine, a Red-Emitting Fluorescent Probe, for Sensitive Quantitative Analysis of RNA at Nanogram Level[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS, 2016, 36(03): 749-754. |
|
|
|
|
